A5101690849- Viral Infectious Diseases and Gene Expression in Insects
- Microbial Metabolic Engineering and Bioproduction
- Plant tissue culture and regeneration
- Biofuel production and bioconversion
- RNA Interference and Gene Delivery
- Enzyme Production and Characterization
- melanin and skin pigmentation
- Heat shock proteins research
- Nanoparticle-Based Drug Delivery
- Enzyme Catalysis and Immobilization
- Protein purification and stability
- 3D Printing in Biomedical Research
- Gene expression and cancer classification
- Immunotherapy and Immune Responses
- Xenotransplantation and immune response
- Transgenic Plants and Applications
- Biochemical Analysis and Sensing Techniques
- Monoclonal and Polyclonal Antibodies Research
- Virus-based gene therapy research
- Fungal and yeast genetics research
- Plant Genetic and Mutation Studies
- Ion channel regulation and function
- Organ Transplantation Techniques and Outcomes
- Advanced Control Systems Optimization
- Bacteriophages and microbial interactions
Nagoya University
2011-2025
Tokyo Metropolitan Komagome Hospital
2014-2025
Hiroshima City Asa Citizens Hospital
2024
Citizens Specialty Hospitals
2024
National Institute of Diabetes and Digestive and Kidney Diseases
2023-2024
ProQuest (United States)
2023-2024
University College London
2023
National Institute of Advanced Industrial Science and Technology
2022
Sapporo Medical University
2001-2021
Ehime University
2007-2018
A single-molecule fluorescence resonance energy transfer (FRET) method has been developed to observe the activation of small G protein Ras at level individual molecules. KB cells expressing H- or K-Ras fused with YFP (donor) were microinjected fluorescent GTP analogue BodipyTR-GTP (acceptor), and epidermal growth factor-induced binding YFP-(H K)-Ras was monitored by FRET. On activation, diffusion greatly suppressed/immobilized, suggesting formation large, activated Ras-signaling complexes....
The effect of hyperthermia on solid glioma tissue formed subcutaneously in the left femoral region female F344 rats was investigated. Magnetite cationic liposomes (MCLs), which have a positive surface charge, were used as heating mediators for intracellular hyperthermia. MCLs injected into tumors, then subjected to irradiation by an alternating magnetic field (118 kHz, 384 Oe). divided four groups, received no (control: group I), or 30 min given once (group II), twice III) three times IV),...
Since the characterization of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) as a major melanogenic intermediate, fate this compound and mechanisms its incorporation into melanin polymer have become issues in study melanogenesis. DHICA is stable dihydroxyindole with low rate spontaneous oxidation, suggesting that enzymatic mechanism(s) might contribute to evolution. The most obvious candidates are melanosomal tyrosinases. We recently shown mouse melanosomes contain two electrophoretically...
Immunotherapy (IT) has become an accepted therapeutic modality. We previously reported that intracellular hyperthermia (IH) using magnetic nanoparticles induces antitumor immunity. undertook these studies in order to study the combined effects of IT and IH on melanoma. Magnetite cationic liposomes (MCLs) have a positive surface charge generate heat alternating field (AMF) due hysteresis loss. MCLs were injected into B16 melanoma nodule C57BL/6 mice, which subjected AMF for 30 min. The...
‘Magnetite cationic liposomes (MCL)’ were developed as a means to generate intracellular hyperthermia. Affinity of the MCL glioma cells was ten times higher than that magnetite‘neutral’ due electrostatic interaction based on positive charge MCL. Heat generation studied using agar phantoms and small pellets rat cells. When high‐frequency magnetic field, 118 kHz, 384 Oe applied in presence MCL, cell pellet 80 μl (5.4 mm diameter) heated over 43°C all died after 40 min irradiation owing...
Mammalian melanogenesis is regulated directly or indirectly by over 85 distinct loci. The<i>Tyr</i>/<i>albino</i> locus, in which mutations cause a lack of pigmentation, encodes tyrosinase (Tyr), the critical and rate-limiting melanogenic enzyme. Other enzymes include Tyrp1 (or TRP1) 3,4-dihydroxyphenylalanine-chrome tautomerase (Dct TRP2) encoded at <i>Tyrp1</i>/<i>brown</i> <i>Dct</i>/<i>slaty</i> loci, respectively. Murine can oxidize 5,6-dihydroxyindole-2-carboxylic acid (DHICA) produced...
Novel technologies for creating three-dimensional constructs with complex shapes would be highly useful in tissue engineering. In the present study, tubular structures were constructed using magnetic force. Magnetite nanoparticles cationic liposomes taken up by target cells. The magnetically labeled cells seeded onto ultralow-attachment plates, and a magnet was placed under wells. After 24 h of culture, formed cell sheet. Subsequently, when cylindrical rolled sheet, sheet attracted to tube...
The sterilizing effect of supercritical carbon dioxide (SC-C02) is described as compared with gaseous C02(G-C02) and liquid C02(L-C02). Baker's yeast, Escherichia coli, Staphylococcus aureus conidia Aspergillus niger were sterilized by treating SC-C02 at 200 atm 35°C when the water content each microorganism was 70 ~ 90%. However, dry cells a 2 10% could not be under same conditions. No from on endospores Bacillus subtilis B. s tear other mophilus observed. G-C02 L-C02 produced no against...
Solubilities of carbon dioxide and ethylene in water a number aqueous salt solutions are measured at about I atm. 25°C by the gas volumetric method. A experimental results correlated modified empirical Setschenow equation. The salting-out parameters which consist three components ascribed to gas, cation anion solution, estimated from this experiment as well those listed International Critical Tables Landolt-Börnstein Tables. With these calculated parameters, solubilities predicted accurately...
Bone marrow-derived mesenchymal stromal cells (BM-MSCs) in culture are derived from BM or skeletal stem cells. Whereas MSCs have been exploited clinical medicine, the identification of MSC-specific markers has limited. Here, we report that a cell surface and secreted protein, Meflin, is expressed cultured MSCs, fibroblasts pericytes, but not other types including epithelial, endothelial smooth muscle In vivo, Meflin by immature osteoblasts chondroblasts. addition, found on distributed...
Induction of antitumor immunity to T‐9 rat glioma by intracellular hyperthermia using functional magnetic particles was investigated. Magnetite cationic liposomes (MCLs), which have a positive surface charge, were used as heating mediators for hyperthermia. Solid tissues formed subcutaneously on both femurs female F344 rats, and MCLs injected via needle only into the left solid tumors (treatment side). The rats then divided two groups, received no irradiation, or irradiation 30 min given...
ABSTRACT Mammalian melanocytes can produce two basic types of melanin, eumelanin and pheomelanin, within discrete organelles termed melanosomes. The physiological signals that regulate this switch are extrinsic to the melanocyte, include α-melanocyte stimulating hormone agouti protein. Tyrosinase, encoded at albino locus, is enzyme essential for synthesis both but other tyrosinase-related proteins (e.g. TRP1 brown locus TRP2 slaty locus) eumelanogenesis catalytically steps distal tyrosinase...
The paracrine linkage of endothelins (ET) between keratinocytes and melanocytes suggested that ETs are intrinsic mediators for human in UVB-induced pigmentation. In this study, the role ET-1 epidermal hyperpigmentation was investigated vivo vitro. addition 10 nM induced a H-7 (10 microM) suppressible-increase tyrosinase activity cultured accompanied by elevated levels tyrosinase-related protein-1 mRNA expression as shown Northern blotting. Analysis signaling mechanisms leading to activation...