A5048038268- Cancer Cells and Metastasis
- Cell Adhesion Molecules Research
- Wnt/β-catenin signaling in development and cancer
- Cancer-related gene regulation
- Corneal surgery and disorders
- Microtubule and mitosis dynamics
- Hedgehog Signaling Pathway Studies
- Neurogenesis and neuroplasticity mechanisms
- Congenital gastrointestinal and neural anomalies
- RNA Research and Splicing
- Cancer, Hypoxia, and Metabolism
- Neuroendocrine Tumor Research Advances
- Glaucoma and retinal disorders
- Genomics and Chromatin Dynamics
- Digestive system and related health
- Axon Guidance and Neuronal Signaling
- Glycosylation and Glycoproteins Research
- Ubiquitin and proteasome pathways
- Renal and related cancers
- Signaling Pathways in Disease
- RNA modifications and cancer
- Corneal Surgery and Treatments
- Nerve injury and regeneration
- TGF-β signaling in diseases
- Fibroblast Growth Factor Research
Metropolitan University
2025
Osaka Metropolitan University
2024-2025
Nagoya University
2015-2024
Fujita Health University
1998-2024
John Wiley & Sons (Germany)
2024
University of Washington
2024
The Retina Center
2024
Fujita Health University Hospital
2020-2023
The Japanese Society of Gastroenterological Surgery
2016-2023
Hiroshima University Hospital
2023
The ret transforming gene was activated by recombination between two unlinked segments of human DNA, most likely during transfection NIH 3T3 cells. To further define this gene, we isolated and sequenced cDNA clones. nucleotide sequence indicates that the active encodes a fusion protein with carboxy-terminal domain which is 40 to 50% homologous members tyrosine kinase family. This preceded hydrophobic characteristic transmembrane domain. Transcription detected in SK-N-SH neuroblastoma, HL-60...
Cancer-associated fibroblasts (CAF) constitute a major component of the tumor microenvironment. Recent observations in genetically engineered mouse models and clinical studies have suggested that there may exist at least two functionally different populations CAFs, is, cancer-promoting CAFs (pCAF) cancer-restraining (rCAF). Although various pCAF markers been identified, identity rCAFs remains unknown because lack rCAF-specific marker(s). In this study, we found Meflin,...
Transforming activity of the c-ret proto-oncogene with multiple endocrine neoplasia (MEN) 2A mutations was investigated by transfection NIH 3T3 cells. Mutant genes driven simian virus 40 or cytomegalovirus promoter induced transformation high efficiencies. The 170-kDa Ret protein present on cell surface transformed cells highly phosphorylated tyrosine and formed disulfide-linked homodimers. This result indicated that MEN ligand-independent dimerization c-Ret surface, leading to activation...
We isolated and sequenced a cDNA clone of the human gene encoded by 5' half ret transforming gene. The nucleotide sequence indicates that it encodes protein with "finger" structures which represent putative metal- nucleic acid-binding domains. Transcription this was detected at high levels in variety rodent tumor cell lines, mouse testis, embryos. In addition, unique transcript observed testis RNA. When expression examined different stages spermatogenesis, striking increase mRNA accompanied...
Most mutations after DNA damage in yeast Saccharomyces cerevisiae are induced by error-prone translesion synthesis employing scRev1 and polymerase zeta that consists of scRev3 scRev7 proteins. Recently, the human REV1 (hREV1) REV3 (hREV3) genes were identified, their products revealed to be involved UV-induced mutagenesis, as observed for counterparts. Human REV7 (hREV7) was also cloned, its product found interact with hREV3, but biological function hREV7 remained unknown. We report here...
Previously, we demonstrated that indoxyl sulphate (IS), a uraemic toxin, induced aortic calcification in hypertensive rats. This study aimed to determine if IS induces the production of reactive oxygen species (ROS) and expression osteoblast-specific proteins human smooth muscle cells (HASMCs).In order achieve these goals, HASMCs were incubated with IS. ROS detected using probes fluorescence detector. The alkaline phosphatase (ALP), osteopontin organic anion transporters (OAT1, OAT3) was...
Germ line mutations of the ret proto-oncogene are associated with development three dominantly inherited neoplastic disorders, multiple endocrine neoplasia (MEN) 2A, MEN 2B, and familial medullary thyroid carcinoma. It has been demonstrated that result in constitutive activation Ret protein, leading to transformation NIH 3T3 cells. In present study we investigated role tyrosine residues carboxyl-terminal sequence for transforming activity 2A or 2B mutation (MEN2A-Ret MEN2B-Ret). Substitution...
Abstract Girdin (girders of actin filaments) is a novel actin-binding Akt substrate that plays an important role in organization and Akt-dependent cell motility fibroblasts. Here, we find expressed variety cancer lines, including the breast line MDA-MB-231, phosphorylated by stimulation insulin-like growth factor (IGF-I). In vitro migration invasion assays showed required for IGF-I–dependent movement MDA-MB-231 cells. Short hairpin interfering RNA directed against markedly inhibited...
Spermatogonial stem cells (SSCs) are required for spermatogenesis. Earlier studies showed that glial cell line-derived neurotrophic factor (GDNF) was indispensable SSC self-renewal by binding to the GFRA1/RET receptor. Mice with mutations in these molecules impaired spermatogenesis, which attributed depletion. Here we show SSCs undergo GDNF-independent self-renewal. A small number of spermatogonia formed colonies when testis fragments from a Ret mutant mouse strain were transplanted into...