A5012903908- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- Axon Guidance and Neuronal Signaling
- Neuropeptides and Animal Physiology
- RNA Interference and Gene Delivery
- Signaling Pathways in Disease
- Neuroscience and Neuropharmacology Research
- Cell death mechanisms and regulation
- Immune Response and Inflammation
- NF-κB Signaling Pathways
- Nuclear Receptors and Signaling
- Congenital heart defects research
- Parkinson's Disease Mechanisms and Treatments
- Pancreatic function and diabetes
- Neuroinflammation and Neurodegeneration Mechanisms
- Liver physiology and pathology
- Cytokine Signaling Pathways and Interactions
- Cellular Mechanics and Interactions
- Neurobiology and Insect Physiology Research
- Retinal Development and Disorders
- RNA Research and Splicing
- Skin and Cellular Biology Research
- Genetics and Neurodevelopmental Disorders
- Alzheimer's disease research and treatments
- Developmental Biology and Gene Regulation
Cardiff University
2012-2021
Trinity College Dublin
2008-2012
St. James's Hospital
2012
Max Planck Society
1986-2007
Max Planck Innovation
2005
University of Edinburgh
1985-2004
Posit Science (United States)
2001-2003
Thomas Jefferson University
2003
University of St Andrews
1993-2001
University of Turin
1997
For survival, embryonic motoneurons in vertebrates depend on as yet undefined neurotrophic factors present the limb bud. Members of neurotrophin family are currently best candidates for such factors, but inactivation their receptor genes leads to only partial loss motoneurons, which suggests that other involved. Glial cell line-derived factor (GDNF), originally identified a trophic specific dopaminergic neurons, was found be 75-fold more potent than neurotrophins supporting survival purified...
To investigate if different neurotrophins regulate the survival of neurons at successive developmental stages, we studied effect nerve growth factor (NGF), brain-derived neurotrophic (BDNF) and neurotrophin-3 (NT-3) on mouse trigeminal closely staged intervals in development. We show that during earliest stages target field innervation display a transitory response to BDNF NT-3. This is lost as become NGF-dependent shortly before neuronal death begins ganglion. NT-3 mRNAs are expressed...
ABSTRACT To understand the role of low-affinity neurotrophin receptor p75 in neural development, we previously generated mice carrying a null mutation locus (Lee, K. F., Li, E., Huber, L. J., Landis, S. C., Sharpe, A. H., Chao, M. V. and Jaenisch, R. (1992) Cell 69, 737 –749). elucidate mechanisms leading to deficits peripheral nervous system mutant mice, have employed dissociated cultures examine responses p75-deficient dorsal root ganglion (DRG) superior cervical (SCG) neurons different...
To determine the spectrum of activity brain-derived neurotrophic factor (BDNF) among first-order sensory neurons, explants nine distinct populations neurons from embryonic chicks 3-14 d incubation (E3-E14) were grown in collagen gels with and without BDNF culture medium. The responded to profuse neurite outgrowth comprised those which are derived neural crest (the dorsomedial part trigeminal ganglion, rostromedial mesencephalic nucleus, jugular lumbosacral dorsal root ganglia) epibranchial...
Abstract An aim of this study was to define precisely the period in developmental history primary sensory neurons during which nerve growth factor (NGF) exerts its growth‐promoting effect. The mouse trigeminal ganglion and peripheral projection were studied at closely staged intervals throughout development using light electron microscopy, influence NGF anti‐NGF antiserum on neurite outgrowth from explants investigated corresponding stages culture. By embryonic day 9.5 (E9.5) fibers first...
The development of the nervous system is a dynamic process during which factors act in an instructive fashion to direct differentiation and survival neurons, induce axonal outgrowth, guidance to, terminal branching within target tissue. Here we report that mice expressing signaling mutants hepatocyte growth factor (HGF) receptor, Met tyrosine kinase, show striking reduction sensory nerves innervating skin limbs thorax, implicating HGF/Met neuron development. Using vitro assays, find HGF...
Cell culture studies have implicated the oxygen-sensitive hypoxia-inducible factor (HIF) prolyl hydroxylase PHD3 in regulation of neuronal apoptosis. To better understand this function vivo, we created PHD3−/− mice and analyzed phenotype. Reduced apoptosis superior cervical ganglion (SCG) neurons cultured from is associated with an increase number cells SCG, as well adrenal medulla carotid body. Genetic analysis by intercrossing HIF-1a+/− HIF-2a+/− demonstrated interaction HIF-2α but not...
The proper growth and elaboration of neural processes is essential for the establishment a functional nervous system during development an integral feature plasticity throughout life. Nuclear factor-kappa B (NF-kappaB) classically known its ubiquitous roles in inflammation, immune stress-related responses regulation cell survival all tissues, including system. NF-kappaB participation other cellular remains poorly understood. Here we report mechanism controlling developing peripheral central...
Activation of brown adipose tissue-mediated thermogenesis is a strategy for tackling obesity and promoting metabolic health. BMP8b secreted by brown/beige adipocytes enhances energy dissipation. Here we show that adipocyte-secreted contributes to adrenergic-induced remodeling the neuro-vascular network in tissue (AT). Overexpression bmp8b AT browning subcutaneous depot maximal thermogenic capacity. Moreover, BMP8b-induced browning, increased sympathetic innervation vascularization were...