A5040755605- Receptor Mechanisms and Signaling
- Neuroscience and Neuropharmacology Research
- Melanoma and MAPK Pathways
- Enzyme Catalysis and Immobilization
- Ion channel regulation and function
- Diet, Metabolism, and Disease
- Neuropeptides and Animal Physiology
- Nicotinic Acetylcholine Receptors Study
- Glycosylation and Glycoproteins Research
- Enzyme Production and Characterization
- Neurotransmitter Receptor Influence on Behavior
- Pharmacological Effects and Assays
- Pancreatic function and diabetes
- Protein Kinase Regulation and GTPase Signaling
- NF-κB Signaling Pathways
- Lipid Membrane Structure and Behavior
- Biochemical Analysis and Sensing Techniques
- Memory and Neural Mechanisms
- Polyamine Metabolism and Applications
- Alcohol Consumption and Health Effects
- Lysosomal Storage Disorders Research
- Neuroendocrine Tumor Research Advances
- Protein Hydrolysis and Bioactive Peptides
- GABA and Rice Research
- Cellular Mechanics and Interactions
Universidad de León
1991-2004
National Institutes of Health
1990-1991
University of Missouri–Kansas City
1989-1990
National Institute of Dental and Craniofacial Research
1990
Universidad de Salamanca
1983-1986
Abstract: Complementary DNAs that encode two forms of the chicken ‐γ‐aminobutyric acid type A (GABA ) receptor β2 subunit have been isolated. These polypeptides differ by presence (β2L) or absence (β2S) 17 amino acids, which contain a possible target for phosphorylation protein kinase C, in large intracellular loop between third and fourth membrane‐spanning domains. The extra sequence β2L is not found previously published GABA β2‐subunit sequences. Analysis genomic DNA has revealed mRNAs...
Abstract: A partial cDNA encoding most of the third intracellular loop mouse α 1d ‐adrenergic receptor subtype was amplified from hippocampus by reverse transcription‐polymerase chain reaction (RT‐PCR) using degenerate oligodeoxynucleotide primers. This DNA fragment used as a probe to isolate an brain library. The deduced amino acid sequence encodes potential protein 562 acids, and northern hybridization poly(A) + RNA isolated detected single 3.0‐kb transcript. Partial fragments 1b ‐ 1a...
Four splice variants (alpha I-IV) of the stress-activated protein kinase JNK/SAPK alpha-isoform have been identified in mouse. One them I) contains an open reading frame 1269 bp encoding a potential 423 amino acids, whereas second variant II) differs region 31 acids located subdomain IX. alpha III lacks this and also terminal portion 3' untranslated (3' UTR). A fourth IV) which 41 IX has identified. These are differentially expressed mouse tissues: I is most abundant brain areas, II mainly...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTInteraction of nicotinic acetylcholine receptor with two monoclonal antibodies recognizing different epitopesMiguel A. Chinchetru, Javier Marquez, Jose C. Garcia-Borron, David P. Richman, and Marino Martinez-CarrionCite this: Biochemistry 1989, 28, 10, 4222–4229Publication Date (Print):May 16, 1989Publication History Published online1 May 2002Published inissue 16...
Abstract: The mechanism by which ethanol affects the γ‐aminobutyric acid (GABA)/benzodiazepine complex is not clear. It known that enhances CI − influx mediated GABA A receptor complex, and although chronic administration does change K D or B max for [ 3 H]flunitrazepam binding, some reports have suggested it could modify modulation of benzodiazepine binding produced GABA. In present work, we studied effect treatment on using light microscopic autoradiography. This technique allows...
Abstract: Equilibrium binding curves were biphasic in control and ethanol‐treated rats. [ 3 H]Muscimol binds to sites of high ( K DA ∼10 n M ) low DB ∼0.3–0.4 µ affinity. Chronic ethanol treatment produced a decrease B maxA value, the hyperbolic profiles progressively affected by chronic vitro treatments, with most this effect corresponding high‐affinity site. IC 50 i values calculated for several competing ligands, using membranes from both animals. The association dissociation also...
THREE α1 adrenergic receptor subtypes have been identified by molecular cloning techniques. However, the detection of mRNA for α1C subtype has not yet reported in rodent tissues. We isolated from rat lung, polymerase chain reaction techniques, a cDNA fragment corresponding to third intracellular domain á1C subtype. The nucleotide and deduced amino acid sequences cloned presented an 87% 96% identity, respectively, with those bovine receptor. Analysis distribution brain shows highest...
A DNA fragment encoding amino acid sequences from the terminal region of mouse serotonin transporter was isolated and sequenced. This is widely distributed throughout brain, as deduced by heminested reverse transcription-polymerase chain reaction (RT-PCR) assays. To identify protein, we have developed specific antibodies against a fusion protein containing its region, domain which shows low degree homology between different neurotransmitter transporters. Western blot analysis brain membranes...
The JNK/SAPK (c-Jun NH2-terminal kinase/stress-activated protein kinase) cascade is activated by a variety of stress stimuli and the inflammatory cytokines interleukin-1 (IL-1) tumor necrosis factor α (TNFα). Four splice variants mouse JNK/SAPKα isoform, which differ in region located subdomains IX–X protein, were previously identified. Analysis sequence central gene indicates that I II are generated typical alternative splicing mechanism, while III IV less common where 3' sites inside an...
Glucosidase II is a key enzyme in the processing of N-glycoproteins since it removes two glucose residues from protein-linked oligosaccharide Glc2Man9GlcNAc2-R. We have studied kinetics purified enzyme, using maltose as substrate. Analysis data fitting to single and double-hyperbolic equations Eadie-Hofstee profile indicate that has binding (active) sites for hydrolysis maltose. The Km Vmax values high-affinity site were 0.43 mM 691 mU/mg, respectively, whereas low-affinity 57.7 2888...