A5081790963- Cancer Cells and Metastasis
- Cell Adhesion Molecules Research
- Wnt/β-catenin signaling in development and cancer
- RNA Research and Splicing
- Cellular Mechanics and Interactions
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Renal and related cancers
- Pancreatic and Hepatic Oncology Research
- interferon and immune responses
- Neurogenesis and neuroplasticity mechanisms
- Virus-based gene therapy research
- Fibroblast Growth Factor Research
- Glioma Diagnosis and Treatment
- Axon Guidance and Neuronal Signaling
- Ovarian cancer diagnosis and treatment
- Hippo pathway signaling and YAP/TAZ
- Cancer-related molecular mechanisms research
- RNA regulation and disease
- Neuroendocrine Tumor Research Advances
- Genetic factors in colorectal cancer
- Histone Deacetylase Inhibitors Research
- Lung Cancer Research Studies
- Circular RNAs in diseases
- Bacterial Genetics and Biotechnology
Kitasato University
2019-2024
The Francis Crick Institute
2016-2023
Tokyo Women's Medical University
2018-2021
Nagoya University
2008-2018
Okayama Shoka University
2018
Okayama University
2018
The Honourable Society of Lincoln's Inn
2016
Kagawa University
2012-2015
Kagoshima University
2011-2013
Oita University
2003
Abstract Girdin (girders of actin filaments) is a novel actin-binding Akt substrate that plays an important role in organization and Akt-dependent cell motility fibroblasts. Here, we find expressed variety cancer lines, including the breast line MDA-MB-231, phosphorylated by stimulation insulin-like growth factor (IGF-I). In vitro migration invasion assays showed required for IGF-I–dependent movement MDA-MB-231 cells. Short hairpin interfering RNA directed against markedly inhibited...
Abstract PI3K–Akt signaling is critical for the development, progression, and metastasis of malignant tumors, but its role in tumor microenvironment has been relatively little studied. Here, we report that Akt substrate Girdin, an actin-binding protein regulates cell migration, expressed activated by phosphorylation cancer-associated fibroblasts (CAF) blood vessels within microenvironment. Lewis lung tumors grafted into mice defective Akt-mediated Girdin (SA transgenic mice) exhibited a...
Bone marrow-derived mesenchymal stromal cells (BM-MSCs) in culture are derived from BM or skeletal stem cells. Whereas MSCs have been exploited clinical medicine, the identification of MSC-specific markers has limited. Here, we report that a cell surface and secreted protein, Meflin, is expressed cultured MSCs, fibroblasts pericytes, but not other types including epithelial, endothelial smooth muscle In vivo, Meflin by immature osteoblasts chondroblasts. addition, found on distributed...
Dishevelled is the common mediator of canonical and non-canonical Wnt signalling pathways, which are important for embryonic development, tissue maintenance cancer progression. In pathway, Rho family small GTPases acting downstream has essential roles in cell migration. The mechanisms by pathway regulates Rac activation remain unknown. Here we show that Daple (Dishevelled-associating protein with a high frequency leucine residues) Wnt5a-mediated formation lamellipodia through interaction...
Central diabetes insipidus (CDI) can be caused by several diseases, but in about half of the patients etiological diagnosis remains unknown. Lymphocytic infundibulo-neurohypophysitis (LINH) is an increasingly recognized entity among cases idiopathic CDI; however, differential from other pituitary diseases including tumors difficult because similar clinical and radiological manifestations. The definite LINH requires invasive biopsy.The study was designed to identify autoantigen(s) thus...
Centrosome amplification is a common feature of human tumors. To survive, cancer cells cluster extra centrosomes during mitosis, avoiding the detrimental effects multipolar divisions. However, it unclear whether clustering requires adaptation or inherent to all cells. Here, we show that have varied abilities centrosomes. Epithelial are innately inefficient at even in presence HSET/KIFC1, which essential but not sufficient promote clustering. The E-cadherin decreases cortical contractility...
In postnatally developing and adult brains, interneurons of the olfactory bulb (OB) are continuously generated at subventricular zone forebrain. The newborn neuroblasts migrate tangentially to OB through a well defined pathway, rostral migratory stream (RMS), where undergo collective migration termed “chain migration.” cell-intrinsic regulatory mechanism neuroblast chain migration, however, has not been uncovered. Here we show that mice lacking actin-binding Akt substrate Girdin (a protein...
Histone deacetylases (HDAC) are involved in carcinogenesis through their regulation of cell proliferation, differentiation, and survival. The inhibitors HDAC exhibit profound synergistic effects cancer treatment when combined with other anticancer drugs. However, the molecular mechanisms underlying this synergy not fully understood. Here, we show that HDAC1 increases resistance cells to oxidative stress by negatively regulating expression thioredoxin binding protein 2 (TBP-2). We found...
Cell migration is a critical cellular process that determines embryonic development and the progression of human diseases. Therefore, cell- or context-specific mechanisms by which multiple promigratory proteins differentially regulate cell must be analyzed in detail. Girdin (girders actin filaments) (also termed GIV, Gα-interacting vesicle associated protein) an actin-binding protein regulates various cells such as endothelial cells, smooth muscle neuroblasts, cancer cells. Here we show...
Human REV 7 (also known as MAD 2L2 and 2B) is involved in DNA repair, cell cycle regulation, gene transcription, carcinogenesis. In this study, we evaluated the expression of epithelial ovarian cancer ( EOC ) analyzed association between its chemosensitivity clear carcinoma CCC cells. Expression human tissues was assessed by immunohistochemical staining. detected majority s (92.0%) with especially high levels frequently observed (73.5%) compared that non‐ (53.4%). Enhanced immunoreactivity...
In the progression of glioma, tumour cells often exploit perivascular microenvironment to promote their survival and resistance conventional therapies. Some these are considered be brain stem (BTSCs); however, molecular nature has not been specifically clarified because complexity glioma. Here, we identified CD109, a glycosylphosphatidylinositol-anchored protein regulator multiple signalling pathways, as critical lower-grade glioma (World Health Organization grade II/III) by...
The Sprouty (SPRY) family of proteins includes important regulators downstream signaling initiated by receptor tyrosine kinases. In the present study, we investigated role SPRY in intracellular via RET kinase activated glial cell line‐derived neurotrophic factor (GDNF). Expression SPRY1, SPRY2, SPRY3 and SPRY4 HEK293T cells transfected with GDNF α1 (GFRα1) genes significantly reduced sustained ERK activation as well ELK‐1 activation. Because expression SPRY2 was efficiently induced TGW human...
For collective invasion, cancer cells form cohesive groups comprised of leading (LCs) at the forefront and following (FCs) rear. However, molecular mechanisms that define LCs FCs remain elusive. Here, we demonstrated LCs, but not FCs, upregulated expression integrin β1 after loss intercellular adhesion. The LC-specific was posttranscriptionally regulated by TRIM27/MRTF-B complex in response to adhesion, thereby regulating stability translation mRNA via microRNA-124 LCs. Accordingly,...
Abstract In clathrin‐mediated endocytosis ( CME ), specificity and selectivity for cargoes are thought to be tightly regulated by cargo‐specific adaptors distinct cellular functions. Here, we show that the actin‐binding protein girdin is a regulator of cargo‐selective . Girdin interacts with dynamin 2, GTP ase excises endocytic vesicles from plasma membrane, functions as its ase‐activating protein. Interestingly, depletion leads defect in clathrin‐coated pit formation center cells. Also,...