A5018776194- Wound Healing and Treatments
- Immune cells in cancer
- Angiogenesis and VEGF in Cancer
- Cancer, Hypoxia, and Metabolism
- Cancer Research and Treatments
- Cardiac tumors and thrombi
- Virus-based gene therapy research
- Ferroptosis and cancer prognosis
- Cell Image Analysis Techniques
- Skin Protection and Aging
- Cancer Immunotherapy and Biomarkers
- interferon and immune responses
- Cell Adhesion Molecules Research
- Breast Cancer Treatment Studies
- Glioma Diagnosis and Treatment
- Cancer, Stress, Anesthesia, and Immune Response
- Immunotherapy and Immune Responses
- HER2/EGFR in Cancer Research
- Esophageal Cancer Research and Treatment
- Peptidase Inhibition and Analysis
- Cancer Cells and Metastasis
- Computational Drug Discovery Methods
- Radiopharmaceutical Chemistry and Applications
- Immune Cell Function and Interaction
- Tendon Structure and Treatment
Institute of Cancer Research
2020-2024
Cancer Research UK
2009-2021
The Francis Crick Institute
2016-2020
Albert Einstein College of Medicine
2013-2018
Cancer Research UK Cambridge Center
2011
Gordon Center for Medical Imaging
2007
Harvard University
2007
Massachusetts General Hospital
2007
Dissemination of tumor cells is an essential step in metastasis. Direct contact between a macrophage, mammalian-enabled (MENA)-overexpressing cell, and endothelial cell [Tumor MicroEnvironment Metastasis (TMEM)] correlates with metastasis breast cancer patients. Here we show, using intravital high-resolution two-photon microscopy, that transient vascular permeability intravasation occur simultaneously exclusively at TMEM. The hyperpermeable nature vasculature described as spatially...
Tumor-associated macrophages (TAMs) are critical for tumor metastasis. Two TAM subsets support cancer cell intravasation: migratory guide cells toward blood vessels, where sessile perivascular assist their entry into the blood. However, little is known about inter-relationship between these functionally distinct TAMs or possible inter-conversion. We show that motile, streaming newly arrived monocytes, recruited via CCR2 signaling, then differentiate macrophages. This unidirectional process...
Local tissue stem cells have been described in airways of the lung but their contribution to normal epithelial maintenance is currently unknown. We therefore developed aggregation chimera mice and a whole-lung imaging method determine relative contributions progenitor (Clara) bronchiolar repair. In moderately injured chimeric patches were small size not associated with previously cell niches. This finding suggested that single, randomly distributed maintain homeostasis. contrast we found...
Abstract The association between tissue damage, chronic inflammation and cancer is well known. However, the underlying mechanisms are unclear. Here we characterize a mouse model in which constitutive epidermal extracellular-signal-regulated kinase-MAP-kinase signalling results inflammation, skin wounding induces tumours. We show that tumour incidence correlates with wound size inflammatory infiltrate. Ablation of necrosis factor receptor (TNFR)-1/-2, Myeloid Differentiation primary response...
In mammalian epidermis, integrin expression is normally confined to the basal proliferative layer that contains stem cells. However, in epidermal hyperproliferative disorders and tumors, integrins are also expressed by suprabasal cells, with concomitant up-regulation of Erk mitogen-activated protein kinase (MAPK) signaling. transgenic mice, activated MAPK 1 (MEK1) suprabasal, nondividing, differentiated cell layers (InvEE transgenics) results hyperproliferation skin inflammation. We now...
Abstract Macrophages are essential for the progression and maintenance of many cancers, but their role during earliest stages tumor formation is unclear. To test this, we used a previously described transgenic mouse model wound-induced skin tumorigenesis, in which expression constitutively active MEK1 differentiating epidermal cells results chronic inflammation (InvEE mice). Upon wounding, number dermal monocytes macrophages increased wild-type InvEE skin, increase was greater, more rapid,...
Abstract Many altered pathways in cancer cells depend on growth factor receptors. In primary malignant gliomas, the amplification/alteration of epidermal receptor (EGFR) has been shown to play a significant role enhancing glioma burden. an effort dissect EGFR expression progression vivo and evaluate targeted therapies for we have genetically engineered visualize dynamics real time vivo. Using lentiviral vectors bearing fusions between its exon 2 7 deleted variant (EGFRvIII) with green...
ERAP1 is a zinc-dependent M1-aminopeptidase that trims lipophilic amino acids from the N-terminus of peptides. Owing to its importance in processing antigens and regulation adaptive immune response, dysregulation highly polymorphic has been implicated autoimmune disease cancer. To test this hypothesis establish role these areas, high affinity, cell permeable selective chemical probes are essential. DG013A 1, phosphinic acid tripeptide mimetic inhibitor with reported low nanomolar affinity...
Abstract Metastasis is a multistep process involving tumor and stromal cells. The microanatomical site consisting of perivascular macrophage interacting with Mena over-expressing cell has been named the “tumor microenvironment metastasis” (TMEM). TMEM density predicts distant metastatic recurrence in breast cancer patients making study function essential. In spontaneous orthotopic mouse mammary tumors (MMTV-PyMT), as progresses to malignancy cells have increased expression assemble TMEM....
Abstract During breast cancer metastasis motile tumor cells migrate within the primary to blood vessels and enter bloodstream which leads these other organs where they can found a new metastasis. These have navigate from their site vessel, make way through extra cellular matrix of basement membranes mammary ducts vessels. Cancer are not acting alone during metastatic process. Tumor associated macrophages (TAMs) play an important role development The on-going research aims gain better...
Abstract Sites of direct contact between a macrophage, tumor cell and endothelial [Tumor MicroEnvironment Metastasis (TMEM)], correlates with metastasis in breast cancer patients independently other clinical prognostic indicators suggesting role for TMEM function hematogenous dissemination. Here we show, using intravital high-resolution two-photon microscopy, that intravasation occurs only at TMEM. Tumor concurrently transient, local vascular permeability an autochthonous mouse mammary...
Supplementary Figure 1 from Visualizing the Dynamics of EGFR Activity and Antiglioma Therapies <i>In vivo</i>
<div>Abstract<p>Many altered pathways in cancer cells depend on growth factor receptors. In primary malignant gliomas, the amplification/alteration of epidermal receptor (EGFR) has been shown to play a significant role enhancing glioma burden. an effort dissect EGFR expression progression <i>in vivo</i> and evaluate targeted therapies for we have genetically engineered visualize dynamics real time vivo</i>. Using lentiviral vectors bearing fusions between its...
Supplementary Figure 1 from Visualizing the Dynamics of EGFR Activity and Antiglioma Therapies <i>In vivo</i>
<div>Abstract<p>Many altered pathways in cancer cells depend on growth factor receptors. In primary malignant gliomas, the amplification/alteration of epidermal receptor (EGFR) has been shown to play a significant role enhancing glioma burden. an effort dissect EGFR expression progression <i>in vivo</i> and evaluate targeted therapies for we have genetically engineered visualize dynamics real time vivo</i>. Using lentiviral vectors bearing fusions between its...
<p>Keratinocyte proliferation in PU.1-YFPxInvEE (InvEE) and PU.1-YFPxWT (WT) skin.</p>
<p>Legend for Supplementary Figures S1-S6.</p>