A5102813374- DNA Repair Mechanisms
- Carcinogens and Genotoxicity Assessment
- Porphyrin Metabolism and Disorders
- melanin and skin pigmentation
- Porphyrin and Phthalocyanine Chemistry
- Electrolyte and hormonal disorders
- Folate and B Vitamins Research
- Metal-Catalyzed Oxygenation Mechanisms
- Fluorine in Organic Chemistry
- Microbial Metabolites in Food Biotechnology
- Neuroendocrine regulation and behavior
- Enzyme Production and Characterization
- Chemical Synthesis and Analysis
- CRISPR and Genetic Engineering
- Skin Protection and Aging
- Genetics, Aging, and Longevity in Model Organisms
- Metabolism and Genetic Disorders
- Light effects on plants
- Cancer Research and Treatments
- RNA Interference and Gene Delivery
- Adrenal Hormones and Disorders
- Cellular Mechanics and Interactions
- Cancer-related Molecular Pathways
- Neuroscience and Neuropharmacology Research
- RNA regulation and disease
Kobe University
2003-2024
University of Fukui
2010-2022
Nagoya University
2014-2018
Niigata University of Pharmacy and Medical and Life Sciences
2003-2007
Osaka University
1996-2004
Fujita Health University
2004
National Cancer Institute
2004
Niigata College of Technology
1992-2001
Tokyo Medical and Dental University
1976
Melanin protects the skin against DNA damage induced by direct absorption of sunlight's UV radiation. Yet, irradiating melanin in vitro or cultured cells also generates active oxygen species such as superoxide, which can indirectly induce oxidative base lesions and strand breaks. This photosensitization is greater for pheomelanin (yellow red melanin) than eumelanin (brown black). The vivo photosensitizing ability unknown. We used congenic mice black, yellow, albino coat colors to investigate...
Hyponatremia is the most common clinical electrolyte disorder. Once thought to be asymptomatic in response adaptation by brain, recent evidence suggests that chronic hyponatremia may linked attention deficits, gait disturbances, risk of falls, and cognitive impairments. Such neurologic defects are associated with a reduction quality life significant cause mortality. However, because underlying diseases such as adrenal insufficiency, heart failure, liver cirrhosis, cancer also affect brain...
Central diabetes insipidus (CDI) can be caused by several diseases, but in about half of the patients etiological diagnosis remains unknown. Lymphocytic infundibulo-neurohypophysitis (LINH) is an increasingly recognized entity among cases idiopathic CDI; however, differential from other pituitary diseases including tumors difficult because similar clinical and radiological manifestations. The definite LINH requires invasive biopsy.The study was designed to identify autoantigen(s) thus...
Palmitoylation is a lipid modification involving the attachment of palmitic acid to cysteine residue, thereby affecting protein function. We investigated effect palmitoylation tyrosinase, rate-limiting enzyme in melanin synthesis, using human three-dimensional skin model system and melanocyte culture. The inhibitor, 2-bromopalmitate, increased content tyrosinase levels melanogenic cells by suppressing degradation. site was Cysteine500 C-terminal cytoplasmic tail tyrosinase....
Phosphatidylinositol 3,4,5-triphosphate (PtdIns(3,4,5)P(3)) accumulates at the leading edge of migrating cells and works, least partially, as both a compass to indicate directionality hub for subsequent intracellular events. However, how PtdIns(3,4,5)P(3) regulates migratory machinery has not been fully elucidated. Here, we demonstrate novel mechanism efficient lamellipodium formation that depends on reciprocal regulation itself. LL5beta, whose subcellular localization is directed by...
To analyze the function of xeroderma pigmentosum group A (XPA) protein in strand-specific DNA repair, we examined repair UV-induced cyclobutane pyrimidine dimer (CPD) transcribed and non-transcribed strands dihydrofolate reductase gene (XP-A) cell line (XP12ROSV) which was transfected with various types mutant XPA cDNA. The transfectant overexpressing a defect interaction either ERCC1, replication (RPA), or general transcription factor TFIIH, showed more less decreased CPD each strand...
Xeroderma pigmentosum (XP) is a rare autosomal recessive hereditary disease. Patients with XP have severe hypersensitivity to sunlight, resulting in skin cancers, and some patients neurological symptoms. In Japan, complementation group A (XP-A) the most common form, it associated We performed nationwide survey on determine present status of Japan. The distribution groups Japan was considerably different from that other countries, but there higher frequency variant type, which similar...
To gain insight for the role of mast cell-produced heparin in regulation epidermal homeostasis and skin pigmentation, we have investigated effect on melanosome uptake proinflammatory responses normal human keratinocytes (NHEKs). We quantified phagocytic activity NHEKs with melanosomes or fluorescent microspheres. Heparin exhibited inhibitory keratinocyte phagocytosis through blocking PI3k/Akt MEK/ERK signaling pathways. In fact, heparin-treated showed impaired activation Akt ERK during...
The key intermediate to squalamine, (5α,7α,24R)-7,24-dihydroxy-cholestan-3-one, was synthesized from the 3-O-acetyl-24R,25-dihydroxy derivative of desmosterol via 10 steps in 16% overall yield and squalamine also prepared two further 7.4% total derivative.
Isolated adrenocorticotropin deficiency (IAD) is characterized by low or absent adrenocorticotropic hormone (ACTH) production. IAD presumed to be caused in part an autoimmune mechanism, and several lines of evidence have suggested the presence anti-pituitary antibodies IAD. However, exact autoantigens remain unknown. The present study was designed identify autoantigen(s) using chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Rat anterior pituitary lysate subjected SDS-PAGE,...
Most patients with xeroderma pigmentosum complementation group D (XP-D) from Western countries suffer neurological symptoms, whereas Japanese display only skin manifestations without symptoms. We have previously suggested that these differences in clinical XP-D are attributed partly to a predominant mutation ERCC2, and the allele frequency of S541R is highest Japan.We diagnosed child mild case by evaluation DNA repair activity whole-genome sequencing, followed her ten years.Skin cancer,...
Summary Background Xeroderma pigmentosum ( XP ) is a photosensitive genodermatosis with increased susceptibility to skin cancers. Patients are typically diagnosed when they consult dermatologist for Case/Methods The genetic analysis and 2–8 years of follow‐up three school‐age patients ‐ V described. were referred us because pigmented freckles; had not experienced abnormal sunburn or developed cancer at their first visit. All harbored mutation in the POLH gene. XPV9KO was age 13 homozygous...