A5008555927- HIV Research and Treatment
- Immune Cell Function and Interaction
- HIV/AIDS drug development and treatment
- HIV/AIDS Research and Interventions
- Marine Biology and Ecology Research
- T-cell and Retrovirus Studies
- Animal Disease Management and Epidemiology
- Herpesvirus Infections and Treatments
- Vector-Borne Animal Diseases
- Cytomegalovirus and herpesvirus research
- Isotope Analysis in Ecology
- T-cell and B-cell Immunology
- Crustacean biology and ecology
- Virus-based gene therapy research
- Bacteriophages and microbial interactions
- Mosquito-borne diseases and control
- Virology and Viral Diseases
- Animal Virus Infections Studies
- Mathematical and Theoretical Epidemiology and Ecology Models
- Hepatitis C virus research
- Monoclonal and Polyclonal Antibodies Research
- Viral gastroenteritis research and epidemiology
- Coral and Marine Ecosystems Studies
- interferon and immune responses
- Marine Ecology and Invasive Species
National Center for Global Health and Medicine
2024-2025
Kyoto University
2014-2023
University of Miyazaki
2002-2017
Infectious Diseases Institute
2010-2014
Japan Science and Technology Agency
2010
Fukushima Medical University
2010
Kagoshima University
1990-2001
The University of Tokyo
1982-1998
Tokyo University of Science
1987-1996
German Primate Center
1996
Isolates of human T-lymphotropic virus type I (HTLV-I) were phylogenetically analyzed from native inhabitants in India and South America (Colombia Chile) Ainu (regarded as pure Japanese descendants the preagricultural "Jomon" period). Their genomes partially sequenced together with isolates Gabon central Africa Ghana West Africa. The phylogenetic tree was constructed sequence data obtained those previously reported HTLV-I simian (STLV-I) isolates. heterogeneity recently recognized, one major...
Blood samples (n=544) from two different populations (Pygmies and Bantus) in Cameroon, West Africa, were analysed. Serological tests indicated that the anti-hepatitis C virus (HCV) prevalence Bantus (20.3 %) was higher than Pygmies (2.3 %, P<0.0001), whereas distribution of hepatitis B (HBV) serological markers equally high both populations: total, 9.4, 17.3 86.8 % for HBsAg, anti-HBs anti-HBc, respectively. HBV genotype A (HBV/A) HBV/E predominant (43.5 each) populations, HBV/D found a...
Two isolates of simian retrovirus related to the human immunodeficiency virus (HIV) were obtained from apparently healthy mandrills, Papio (Mandrillus) sphinx, in western equatorial Africa. This virus, designated SIVMND (simian mandrills), appeared morphologically similar HIV by electron microscopy, showed Mg2+-dependent reverse transcriptase activity, and induced cytopathic effect CD4-positive cells. Western blotting (immunoblotting) analyses revealed that gag pol products cross-reactivity...
We constructed an infectious molecular clone of the human immunodeficiency virus type 2 (HIV-2) and generated nine frameshift mutants corresponding to open reading frames identified so far. Three structural (gag, pol, env) two regulative (tat, rev) gene were not infectious, whereas vif, vpx, vpr, nef genes dispensable for infectivity. All except env rev cytopathic in CD4+ leukemia cells. In transfection assays, expression HIV-2 long terminal repeat was activated by clones HIV-1, HIV-2,...
Dengue virus (DENV) infectious disease is a major public health problem worldwide; however, licensed vaccines or specific antiviral drugs against this infection are not available. To identify novel anti-DENV compounds, we screened 1280 pharmacologically active compounds using focus reduction assay. Bromocriptine (BRC) was found to have potent activity and low cytotoxicity (half maximal effective concentration [EC50], 0.8-1.6 μM; half [CC50], 53.6 μM). Time-of-drug-addition...
ABSTRACT Here we describe, for the first time, recombinants between two highly divergent major groups of human immunodeficiency virus type 1 (HIV-1), M and O, within a Cameroonian woman infected with three different HIV-1 strains, group O virus, subtype D recently reported IBNG (A/G)-like recombinant virus. Using nested extra-long PCR amplification, sequenced from pol region to env including accessory genes viral genome obtained patient’s uncultured peripheral blood mononuclear cells...
The time elapsed between successful cell infection and the start of virus production is called eclipse phase. Its duration specific to each strain and, along with an effective rate, plays a key role in kinetics. How phase varies amongst cells infected same therefore how best mathematically represent its not clear. Most mathematical models either neglect this or assume it exponentially distributed, such that at least some if all can produce immediately upon infection. Biologically,...
Current in vitro cell culture studies of viral replication deliver detailed time courses several virological variables, like the amount virions and number target cells, measured over days experiment. Each these points solely provides a snap-shot virus infection kinetics is brought about by complex interplay infection, production death. It remains challenge to interpret data quantitatively reveal underlying processes understand how depends on kinetic properties. In order decompose we...
Dengue virus (DENV) is transmitted to humans by Aedes mosquitoes, and a public health issue worldwide. No antiviral drugs specific for treating dengue infection are currently available. To identify novel DENV inhibitors, we analyzed library of 95 compounds 120 extracts derived from crude (herbal medicines). In the primary screening, A549 cells infected with DENV-1 were cultured in presence each compound extract at final concentration 10 μM (compound) 100 μg/mL (extract), reduction viral...
Abstract The duration of the eclipse phase, from cell infection to production and release first virion progeny, immediately followed by virus-production last are important steps in a viral infection, setting pace progression modulating response antiviral therapy. Using mathematical model (MM) data for HSC-F cells with SHIV vitro , we reconfirm our earlier finding that phase follows fat-tailed distribution, lasting 19 h (18–20 h). Most importantly, time, show virus-producing duration, which...
A new classification is proposed for the Japanese species of genus Lumbrinerides Orensanz, 1973 (Annelida: Lumbrineridae), based on observation as many specimens possible so to determine extent intra-populational variation each morphological character. Morphological differences apparent in material from ten local sites were compared statistically among 16 tentative, operational taxonomic units, morphologically recognizable units then being subjected a consideration with other known species....
From what viruses the human immunodeficiency (HIVs) originated is an extremely controversial question. To address this question, we have analyzed nucleotide sequences of simian (SIVs) and HIVs by using techniques for understanding molecular evolution. In particular, compared whole genomes, gene region region, between a given pair viruses, including four types SIVs--isolated from mandrills (Papio sphinx), African green monkeys (Cercopithecus aethiops), sooty mangabeys (Cercocebus atys),...
ABSTRACT Human immunodeficiency virus type 1 (HIV-1) replication in macaque cells is restricted mainly by antiviral cellular APOBEC3, TRIM5α/TRIM5CypA, and tetherin proteins. For basic clinical HIV-1/AIDS studies, efforts to construct macaque-tropic HIV-1 (HIV-1mt) have been made us others. Although rhesus macaques are commonly successfully used as infection models, no derivatives suitable for vivo research available date. In this study, obtain novel HIV-1mt clones that resistant major...
Developing a quantitative understanding of viral kinetics is useful for determining the pathogenesis and transmissibility virus, predicting course disease, evaluating effects antiviral therapy. The availability data in clinical, animal, cell culture studies, however, has been quite limited. Many studies virus infection have based solely on measures total or infectious count. Here, we introduce new mathematical model which tracks both load, as well fraction infected uninfected cells within...
A chimeric human and simian immunodeficiency virus carrying the tat, rev, vpu, env, nef genes of type 1 was generated. The virus, NM-3n, grew competently in peripheral blood mononuclear cells from cynomolgus monkeys like parental SIVmac. Two one rhesus monkey inoculated with NM-3n raised antibodies to SIVmac Gag HIV-1 Env. persisted for at least 1.7 years. contained neutralizing activity not only original but also HIV-1. Infectious viruses were isolated 37 63 weeks after inoculation...
We designed a universal primer (UNIPOL) for DNA amplification of AIDS-related viruses. The phylogenetic tree constructed from the presumed sequences amplified with UNIPOL was representative calculated whole pol gene so far reported. able to amplify all four major groups primate lentiviruses and also that distinct virus Ghanaian patient an complex, designated GH-2. This strain scarcely hybridizes known HIV/simian immunodeficiency (SIV) probes. Sequence analysis only fragment revealed rapidly...
Genetic variability in human immunodeficiency virus type 1 (HIV-1) has been studied extensively, but the total nucleotide sequence of HIV-2 genome reported only two strains. For phylogenetic analyses HIV, genetic should be investigated. This paper reports complete an isolate from Ghana, HIV-2[GH-1], showed approximately 85% homology overall with HIV-2ROD. The amino acid gag and pol proteins HIV-2[GH-1] 90% those HIV-2ROD, its env gene central regions were highly variable (more than 20%...
A better understanding of virological events during the early phase human immunodeficiency virus 1 (HIV-1) infection is important for development effective antiviral vaccines. In this study, by using quantitative PCR and an infectious plaque assay, distribution replication were examined in various internal organs rhesus macaques almost month after intrarectal inoculation a pathogenic simian virus/HIV chimeric (SHIV-C2/1-KS661c). At 3 days post-inoculation (p.i.), proviral DNA was detected...