A5020081192- Influenza Virus Research Studies
- COVID-19 epidemiological studies
- Respiratory viral infections research
- Immune Cell Function and Interaction
- HIV Research and Treatment
- COVID-19 Pandemic Impacts
- HIV/AIDS drug development and treatment
- COVID-19 and Mental Health
- SARS-CoV-2 and COVID-19 Research
- Mathematical and Theoretical Epidemiology and Ecology Models
- Plant Virus Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Viral Infections and Outbreaks Research
- Polyomavirus and related diseases
- Bacterial Infections and Vaccines
- COVID-19 impact on air quality
- Animal Disease Management and Epidemiology
- Hepatitis B Virus Studies
- Pulsars and Gravitational Waves Research
- Astrophysical Phenomena and Observations
- Hepatitis C virus research
- Black Holes and Theoretical Physics
- Receptor Mechanisms and Signaling
Grand Valley State University
2020-2024
University of Waterloo
2020-2021
Toronto Metropolitan University
2010-2021
ABSTRACT The H275Y amino acid substitution of the neuraminidase gene is most common mutation conferring oseltamivir resistance in N1 subtype influenza virus. Using a mathematical model to analyze set vitro experiments that allow for full characterization viral replication cycle, we show primary effects on pandemic H1N1 (H1N1pdm09) strain are lengthen mean eclipse phase infected cells (from 6.6 9.1 h) and decrease (by 7-fold) burst size, i.e., total number virions produced per cell. We also...
For a typical influenza infection in vivo, viral titers over time are characterized by 1–2 days of exponential growth followed an decay. This simple dynamic can be reproduced broad range mathematical models which makes model selection and the extraction biologically-relevant parameters from experimental data difficult. We analyze vitro literature, specifically that single-cycle yield experiments, to narrow realistic infection. In particular, we demonstrate viability using normal or lognormal...
In 2007, the A/Brisbane/59/2007 (H1N1) seasonal influenza virus strain acquired oseltamivir-resistance mutation H275Y in its neuraminidase (NA) gene. Although previous studies had demonstrated that this impaired replication capacity of vitro and vivo, oseltamivir-resistant mutant completely out-competed wild-type (WT) was, 2008–2009 season, primary A/H1N1 circulating strain. Using a combination plaque viral yield assays, simple mathematical model, approximate values were extracted for two...
The 2009 pandemic H1N1 (H1N1pdm09) influenza virus is naturally susceptible to neuraminidase (NA) inhibitors, but mutations in the NA protein can cause oseltamivir resistance. H275Y and I223V amino acid substitutions of H1N1pdm09 strain have been separately observed patients exhibiting oseltamivir-resistance. Here, we apply mathematical modelling techniques compare fitness wild-type relative each these two mutants. We find that both background significantly lengthen duration eclipse phase...
Developing a quantitative understanding of viral kinetics is useful for determining the pathogenesis and transmissibility virus, predicting course disease, evaluating effects antiviral therapy. The availability data in clinical, animal, cell culture studies, however, has been quite limited. Many studies virus infection have based solely on measures total or infectious count. Here, we introduce new mathematical model which tracks both load, as well fraction infected uninfected cells within...
Oseltamivir-resistant A/H3N2 influenza isolates with or without the E119V and I222V neuraminidase (NA) mutations were recovered from an immunocompromised patient. Based on plaque size, yield assays, NA activity, impaired viral fitness of mutant was partially restored by mutation.
Experimentation in vitro is a vital part of the process by which clinical and epidemiological characteristics particular influenza virus strain are determined. We detail considerations must be made designing appropriate theoretical/mathematical models these experiments show how modeling can increase information output such experiments. Starting from traditional system ordinary differential equations, common to infectious disease modeling, we broaden approach using an agent-based model,...
Mathematical modelling has successfully been used to provide quantitative descriptions of many viral infections, but for the Ebola virus, which requires biosafety level 4 facilities experimentation, can play a crucial role. efforts have primarily focused on in vivo virus kinetics, e.g., animal models, aid development antivirals and vaccines. But, thus far, these studies not yielded detailed specification infection cycle, could foundational description kinetics deeper understanding their...
The SARS-CoV-2 pandemic has caused significant mortality and morbidity worldwide, sparing almost no community. As the disease will likely remain a threat for years to come, an understanding of precise influences human demographics settlement, as well dynamic factors climate, susceptible depletion, intervention, on spread localized epidemics be vital mounting effective response. We consider entire set local in United States; broad selection demographic, population density, climate factors;...
Developing a quantitative understanding of viral kinetics is useful for determining the pathogenesis and transmissibility virus, predicting course disease, evaluating effects antiviral therapy. The availability data in clinical, animal, cell culture studies, however, has been quite limited. Many studies virus infection have based solely on measures total or infectious count. Here, we introduce new mathematical model which tracks both load, as well fraction infected uninfected cells within...
Background For a typical influenza infection in vivo, viral titers over time are characterized by 1–2 days of exponential growth followed an decay. This simple dynamic can be reproduced broad range mathematical models which makes model selection and the extraction biologically-relevant parameters from experimental data difficult. Results We analyze vitro literature, specifically that single-cycle yield experiments, to narrow realistic infection. In particular, we demonstrate viability using...
The 2009 pandemic H1N1 (H1N1pdm09) influenza virus is naturally susceptible to neuraminidase (NA) inhibitors, but mutations in the NA protein can cause oseltamivir resistance. H275Y and I223V amino acid substitutions of H1N1pdm09 strain have been separately observed patients exhibiting oseltamivir-resistance. Here, we apply mathematical modelling techniques compare fitness wild-type relative each these two mutants. We find that both background significantly lengthen duration eclipse phase...
Strongly gravitating systems can undergo unusual orbital trajectories. For example, “extreme mass ratio inspirals” (observable in the megahertz band by space-based gravitational wave detectors) exhibit “zoom-whirl” orbits, which make complicated waveforms that are useful for mapping out system's structure. Zoom-whirl behavior be intuitively understood context of effective potentials, should familiar to students from classical theory mechanics. Here, we explore zoom-whirl orbits using...
Background For a typical influenza infection in vivo, viral titers over time are characterized by 1–2 days of exponential growth followed an decay. This simple dynamic can be reproduced broad range mathematical models which makes model selection and the extraction biologically-relevant parameters from experimental data difficult. Results We analyze vitro literature, specifically that single-cycle yield experiments, to narrow realistic infection. In particular, we demonstrate viability using...
The 2009 pandemic H1N1 (H1N1pdm09) influenza virus is naturally susceptible to neuraminidase (NA) inhibitors, but mutations in the NA protein can cause oseltamivir resistance. H275Y and I223V amino acid substitutions of H1N1pdm09 strain have been separately observed patients exhibiting oseltamivir-resistance. Here, we apply mathematical modelling techniques compare fitness wild-type relative each these two mutants. We find that both background significantly lengthen duration eclipse phase...
The SARS-CoV-2 pandemic has caused significant mortality and morbidity worldwide, sparing almost no community. As the disease will likely remain a threat for years to come, an understanding of precise influences human demographics settlement, as well dynamic factors climate, susceptible depletion, intervention, on spread localized epidemics be vital mounting effective response. We consider entire set local in United States; broad selection demographic, population density, climate factors;...
Background Developing a quantitative understanding of viral kinetics is useful for determining the pathogenesis and transmissibility virus, predicting course disease, evaluating effects antiviral therapy. The availability data in clinical, animal, cell culture studies, however, has been quite limited. Many studies virus infection have based solely on measures total or infectious count. Here, we introduce new mathematical model which tracks both load, as well fraction infected uninfected...
Background Developing a quantitative understanding of viral kinetics is useful for determining the pathogenesis and transmissibility virus, predicting course disease, evaluating effects antiviral therapy. The availability data in clinical, animal, cell culture studies, however, has been quite limited. Many studies virus infection have based solely on measures total or infectious count. Here, we introduce new mathematical model which tracks both load, as well fraction infected uninfected...
Background Developing a quantitative understanding of viral kinetics is useful for determining the pathogenesis and transmissibility virus, predicting course disease, evaluating effects antiviral therapy. The availability data in clinical, animal, cell culture studies, however, has been quite limited. Many studies virus infection have based solely on measures total or infectious count. Here, we introduce new mathematical model which tracks both load, as well fraction infected uninfected...