A5069668443- Influenza Virus Research Studies
- Immune Cell Function and Interaction
- HIV Research and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Viral Infections and Outbreaks Research
- Respiratory viral infections research
- COVID-19 epidemiological studies
- SARS-CoV-2 and COVID-19 Research
- T-cell and B-cell Immunology
- Hepatitis B Virus Studies
- Mathematical and Theoretical Epidemiology and Ecology Models
- Fungal and yeast genetics research
- Protein Kinase Regulation and GTPase Signaling
- Evolution and Genetic Dynamics
- Gamma-ray bursts and supernovae
- Mosquito-borne diseases and control
- Organometallic Complex Synthesis and Catalysis
- Synthesis and characterization of novel inorganic/organometallic compounds
- Organometallic Compounds Synthesis and Characterization
- Histone Deacetylase Inhibitors Research
- Cytomegalovirus and herpesvirus research
- Pulsars and Gravitational Waves Research
- Astrophysical Phenomena and Observations
- Cellular Mechanics and Interactions
- Wound Healing and Treatments
Toronto Metropolitan University
2011-2021
Los Alamos National Laboratory
2018-2021
Rapid detection of compact binary coalescence (CBC) with a network advanced gravitational-wave detectors will offer unique opportunity for multi-messenger astronomy. Prompt alerts the astronomical community might make it possible to observe onset electromagnetic emission from CBC. We demonstrate computationally practical filtering strategy that could produce early-warning triggers before gravitational radiation final merger has arrived at detectors.
Treatment of HIV infection with either antiretroviral (ARV) therapy or neutralizing monoclonal antibodies (NAbs) leads to a reduction in plasma virus. Both ARVs and NAbs prevent new rounds viral infection, but may have the additional capacity accelerate loss virus-infected cells through Fc gamma receptor (FcγR)-mediated effector functions, which should affect kinetics plasma-virus decline. Here, we formally test role function vivo by comparing rate timing clearance response single-dose...
Significance Virus-neutralizing monoclonal antibodies have been tested for the treatment or prevention of HIV-1 infection. Such an antibody can block virus infectivity and mediate killing virus-infected cells by Fc-mediated effector functions. The relative contributions these two activities in vivo not quantified previously. By quantitatively analyzing results from experiments conducted HIV-1–infected humanized mice SHIV-infected rhesus macaques, we determined that functions contribute about...
In 2007, the A/Brisbane/59/2007 (H1N1) seasonal influenza virus strain acquired oseltamivir-resistance mutation H275Y in its neuraminidase (NA) gene. Although previous studies had demonstrated that this impaired replication capacity of vitro and vivo, oseltamivir-resistant mutant completely out-competed wild-type (WT) was, 2008–2009 season, primary A/H1N1 circulating strain. Using a combination plaque viral yield assays, simple mathematical model, approximate values were extracted for two...
Objectives: Human challenge studies (HCS) are used to evaluate new antiviral treatments and establish clinical proof of concept. Typically, a mean viral load (VL) profile is presented as key figure in therapy publications. In contrast fixed dosing times relative the (inoculation), study design for RSV (respiratory syncytial virus) models includes triggered (i.e., variable time based on qualitative PCR positivity post-challenge) which adds layer complexity information encoded. We sought...
Experimentation in vitro is a vital part of the process by which clinical and epidemiological characteristics particular influenza virus strain are determined. We detail considerations must be made designing appropriate theoretical/mathematical models these experiments show how modeling can increase information output such experiments. Starting from traditional system ordinary differential equations, common to infectious disease modeling, we broaden approach using an agent-based model,...
Inducing latency reversal to reveal infected cells the host immune system represents a potential strategy cure HIV infection. In separate studies, we have previously shown that CD8+ T may contribute maintenance of viral and identified novel SMAC mimetic/IAP inhibitor (AZD5582) capable reversing HIV/SIV in vivo by activating non-canonical (nc) NF-κB pathway. Here, use AZD5582 combination with antibody-mediated depletion CD8α+ further evaluate role maintenance. Six rhesus macaques (RM) were...
A defective interfering particle (DIP) in the context of influenza virus is a virion with significantly shortened RNA segment substituting one eight full-length parent segments, such that it preferentially amplified. Hence, cell co-infected DIPs will produce mainly DIPs, suppressing infectious yields and affecting infection kinetics. Unfortunately, quantification contained sample difficult because they are indistinguishable from standard (STV). Using mathematical model, we investigated...
Wounded cells such as Xenopus oocytes respond to damage by assembly and closure of an array actin filaments myosin-2 controlled Rho GTPases, including Cdc42. Cdc42 are patterned around wounds in a characteristic manner, with active concentrating ring-like zone inside larger, How this patterning is achieved unknown, but at subject regulation other proteins, the protein kinases C. Specifically, activity enhanced PKCβ inhibited PKCη. We adapt mathematical model Simon coworkers probe possible...
Mathematical modelling has successfully been used to provide quantitative descriptions of many viral infections, but for the Ebola virus, which requires biosafety level 4 facilities experimentation, can play a crucial role. efforts have primarily focused on in vivo virus kinetics, e.g., animal models, aid development antivirals and vaccines. But, thus far, these studies not yielded detailed specification infection cycle, could foundational description kinetics deeper understanding their...
Mathematical models (MMs) have been used to study the kinetics of influenza A virus infections under antiviral therapy, and characterize efficacy antivirals such as neuraminidase inhibitors (NAIs). NAIs prevent viral from cleaving sialic acid receptors that bind progeny surface infected cells, thereby inhibiting their release, suppressing infection spread. When treatment with NAIs, MMs represent release implicitly part replication. Consequently, in do not act specifically exclusively on...
This tutorial introduces participants to the design and implementation of an agent-based model using NetLogo through one two different projects: modelling T cell movement within a lymph node or progress viral infection in vitro culture monolayer. Each project is broken into series incremental steps increasing complexity. step described detail code type initially provided. However, each has room grow complexity biological realism so are encouraged expand their beyond scope develop own.
In this work, two studies were performed where mathematical models (MM) used to re-examine and refine quantitative methods based on in vitro assays of influenza A virus infections. the first study, we investigated standard experimental method for counting defective interfering particles (DIPs) reduction (STV) yield (Bellett & Cooper, 1959). We found is valid DIPs provided that: (1) a STV-infected cell’s co-infection window approximately half its eclipse phase (it blocks infection by...