A5101831582- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Antibiotic Resistance in Bacteria
- HIV Research and Treatment
- DNA and Nucleic Acid Chemistry
- HIV/AIDS drug development and treatment
- Genomic variations and chromosomal abnormalities
- Bacterial Genetics and Biotechnology
- Liver Diseases and Immunity
- Gut microbiota and health
- Biopolymer Synthesis and Applications
- Nosocomial Infections in ICU
- Photochemistry and Electron Transfer Studies
- Digestive system and related health
- FOXO transcription factor regulation
- Phagocytosis and Immune Regulation
- Genomics and Chromatin Dynamics
- Oral microbiology and periodontitis research
- Semiconductor materials and devices
- Tuberculosis Research and Epidemiology
- Bacteriophages and microbial interactions
- Pneumonia and Respiratory Infections
- Antibiotics Pharmacokinetics and Efficacy
- Liver Disease Diagnosis and Treatment
Keio University
2018-2023
Keio University Hospital
2022
Nagasaki University
1995-2016
OncoTherapy Science (Japan)
2016
Kyushu University
2012-2015
Japan Science and Technology Agency
2012-2014
Nagasaki University Hospital
2014
The University of Tokyo
2007-2013
Institute for Molecular Science
2009-2011
Tokushima University
2010
Abstract Non-alcoholic steatohepatitis (NASH) is a leading cause of chronic liver disease that can progress to fibrosis. Recent clinical advance suggests reversibility fibrosis, but the cellular and molecular mechanisms underlying NASH resolution remain unclarified. Here, using murine diet-induced subsequent model, we demonstrate direct roles CD8 + tissue-resident memory T (CD8 Trm) cells in resolving Single-cell transcriptome analysis FACS revealed CD69 CD103 − Trm cell enrichment livers....
A technique for replication R- and G-banding of mouse lymphocyte chromosomes was developed, the R-banding pattern analyzed. Optimal banding patterns were obtained with thymidine- BrdU-treatment lymphocytes in same cell cycle. This produced R-band that complete reverse G-band on all chromosomes. Replication methods can be used conjunction nonisotopic, fluorescence situ hybridization (FISH) to localize cloned probes specific chromosomal bands With these complement factor H gene...
The intricate interplay between gut microbes and the onset of experimental autoimmune encephalomyelitis (EAE) remains poorly understood. Here, we uncover remarkable similarities CD4+ T cells in spinal cord their counterparts small intestine. Furthermore, unveil a synergistic relationship microbiota, particularly enriched with tryptophan metabolism gene EC:1.13.11.11, intestinal cells. This symbiotic collaboration results biosynthesis kynurenic acid (KYNA), which modulates recruitment...
Bladder cancer is the second most common genitourinary worldwide, yet its oncogenic origins remain poorly understood. The cancer-testis antigen DEPDC1 was shown recently to contribute bladder oncogenesis. In this study, we examined biological functions of and defined a potential therapeutic strategy target molecule. Coimmunoprecipitation immunocytochemistry revealed that interacted colocalized with zinc finger transcription factor ZNF224, known transcriptional repressor. Inhibiting...
Molecular chirality of amino acids is linked to bacterial recognition by mammals modulate IgA for symbiosis.
CD4+Foxp3+ regulatory T cells (Tregs) are essential for homeostasis in the colon, but mechanism by which local environmental cues determine localization of colonic Tregs is unclear. Here, we administer indigo naturalis (IN), a nontoxic phytochemical aryl hydrocarbon receptor (AhR) agonist used treating patients with ulcerative colitis (UC) Asia, and show that IN increases Helios+ MHC class II+ epithelial (ECs) colon. Interactions between ECs occur mainly near crypt bottom steady state,...
ABSTRACT Here we describe, for the first time, recombinants between two highly divergent major groups of human immunodeficiency virus type 1 (HIV-1), M and O, within a Cameroonian woman infected with three different HIV-1 strains, group O virus, subtype D recently reported IBNG (A/G)-like recombinant virus. Using nested extra-long PCR amplification, sequenced from pol region to env including accessory genes viral genome obtained patient’s uncultured peripheral blood mononuclear cells...
Ultrafast intersystem crossing of UVA-sensitive 4-thiothymidine in aqueous solution was investigated by pump−probe transient absorption. 4-Thiothymidine the excited singlet state exhibited a distinctively different photophysical pathway from that thymidine, with to triplet manifold being dominant and formation completed within approximately 10 ps. This further supported quantum chemical calculations. We propose unique behavior photoexcited results its distinctive molecular electronic...
Pseudomonas aeruginosa bacteremia occurs mainly in immunocompromised patients. However, P. immunocompetent patients has also been reported. The aim of this study was to evaluate the clinical characteristics relation immune status medical records 126 adult with Nagasaki University Hospital were retrospectively reviewed between January 2003 and December 2012. Of bacteremia, 60 (47.6%) classified as immunocompetent. Mortality tended be lower than (7-day mortality, 8% vs. 30%, P < 0.01; 30-day...
Mast cells play a key role in the induction of anaphylaxis, life-threatening IgE-dependent allergic reaction, by secreting chemical mediators that are stored secretory granules. Degranulation mast is triggered aggregation high-affinity IgE receptor, FcεRI, and involves dynamic rearrangement microtubules. Although much known about proximal signals downstream distal signaling events controlling microtubule dynamics remain elusive. Here we report DOCK5, an atypical guanine nucleotide exchange...
Psoriasis and inflammatory bowel disease (IBD) are both chronic diseases occurring in the skin gut, respectively. It is well established that psoriasis IBD have high concordance rates, similar changes immune cells microbiome composition been reported conditions. To study this connection, we used a combination murine model of psoriatic dermatitis colitis which mice were treated topically with Toll-like receptor 7 agonist imiquimod (IMQ) fed dextran sulfate sodium (DSS).We applied IMQ to B6...
The thymus has spatially distinct microenvironments, the cortex and medulla, where developing T-cells are selected to mature or die through interaction with thymic stromal cells. To establish immunological self in thymus, medullary epithelial cells (mTECs) express diverse sets of tissue-specific self-antigens (TSAs). This ectopic expression TSAs largely depends on transcriptional regulator Aire, yet mechanism controlling Aire itself remains unknown. Here, we show that Jmjd6, a dioxygenase...
// Suyoun Chung 1 , Kyoko Kijima Aiko Kudo Yoshiko Fujisawa Yosuke Harada Akiko Taira Naofumi Takamatsu Takashi Miyamoto Yo Matsuo and Yusuke Nakamura 2 OncoTherapy Science, Inc., Kawasaki, Kanagawa, Japan Department of Medicine Surgery, The University Chicago, IL, USA Correspondence to: Nakamura, email: Keywords : MELK, xenograft model, kinase inhibitor, molecular pharmacology, biomarker Received December 22, 2015 Accepted February 08, 2016 Published 24, Abstract MELK is upregulated in...
Intestinal intraepithelial lymphocytes (IELs), the first line of defense against microbial and dietary antigens, are classified as natural or induced based on their origin receptor expression. Induced CD4+CD8αα+TCRβ+ T cells (double positive, DPIELs) originated from CD4+CD8α-TCRβ+ (single SPIELs) increase with aging. However, metabolic requirements metabolic-related genes in IEL development remain unclear. We determined that compartment is hypoxic presence microbes DPIELs increased more than...
Acinetobacter baumannii is one of the main pathogens that cause ventilator-associated pneumonia (VAP) and associated with a high rate mortality. Little known about efficacy macrolides against A. baumannii. In order to confirm azithromycin (AZM) VAP caused by multidrug-resistant (MDRAB), we used mouse model mimics placement plastic tube in bronchus. AZM (10 100 mg/kg body weight) was administered subcutaneously every 24 h beginning at 3 after inoculation. Phosphate-buffered saline as control....
Rifabutin-based regimens are used as rescue therapy for refractory Helicobacter pylori infection; however, the duration which treatment is required and side effects concerning. This study assessed efficacy safety of 7-day rifabutin, amoxicillin, vonoprazan triple third- or later-line H. infection.Patients who did not respond to second-line were enrolled. After infection was confirmed with culture method, patients received rifabutin-containing (20 mg b.i.d., 500 amoxicillin q.i.d., 150...
To assess the molecular epidemiology of HIV-1 in Republic Congo (Congo), we investigated 29 HIV-1s obtained from 82 Congolese AIDS and ARC patients 1996 1997. Part env region including V3 loop was phylogenetically analyzed. The genotypes observed were varied: specimens, 12 (41%) subtype A, 1 (3%) D, 6 (21%) G, H, 2 (7%) J, could not be classified as any known subtypes (U, unclassified). heterogeneous profile HIV1 infection different profiles neighboring Central African countries. These data...
Fusobacterium nucleatum is one of the most common anaerobic bacteria in periodontitis and responsible for several extraoral infections, including respiratory tract diseases. In this study, we examined whether F. induces mucin secretion airway epithelial cells. We also effects macrolides on nucleatum-induced mucus production compared with other antibiotics that exert anti-anaerobic activities. The MUC5AC, major core protein secreted from surface epithelium, bronchial cells after stimulation...