A5044497202- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Pneumonia and Respiratory Infections
- Antibiotic Resistance in Bacteria
- Antimicrobial Resistance in Staphylococcus
- Antibiotics Pharmacokinetics and Efficacy
- Bacterial Identification and Susceptibility Testing
- Pneumocystis jirovecii pneumonia detection and treatment
- Infectious Diseases and Mycology
- Nail Diseases and Treatments
- Influenza Virus Research Studies
- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- Antibiotic Use and Resistance
- Respiratory viral infections research
- Bacterial biofilms and quorum sensing
- Nosocomial Infections in ICU
- SARS-CoV-2 and COVID-19 Research
- Streptococcal Infections and Treatments
- Mycobacterium research and diagnosis
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Legionella and Acanthamoeba research
- Cystic Fibrosis Research Advances
- Bacterial Infections and Vaccines
- Infectious Diseases and Tuberculosis
Osaka Metropolitan University
2022-2025
Tokyo Metropolitan University
2022-2025
Osaka International University
2024-2025
Osaka City University
2014-2023
Osaka City University Hospital
2016-2023
Nagoya University Hospital
2022
Université Laval
2022
Institute for Biomedical and Pharmaceutical Research
2022
University of Bonn
2022
The University of Texas MD Anderson Cancer Center
2022
We compared the specificities and sensitivities of four tests used for serodiagnosis candidemia in 39 patients with candidemia, including 10 superficial Candida colonization, deep mycosis, 20 healthy subjects. The results obtained by dot immunoblotting assay detecting enolase antigen (48 kDa) were those assays mannan antigen, heat-labile (a threshold titer times), beta-glucan (> or = 60 pg/ml). Enolase was detected 28 (71.8%) while 30 (76.9%), (25.6%), 27 (84.4%) positive Cand-Tec assay,...
ABSTRACT This is the first report of a detailed relationship between triazole treatment history and MICs for 154 Aspergillus fumigatus clinical isolates. The duration itraconazole dosage increased as MIC increased, positive correlation was observed ( r = 0.5700, P < 0.0001). number itraconazole-naïve isolates dramatically decreased particularly exceeding 2 μg/ml (0.5 versus μg/ml, 0.03). We also examined cumulative usage other azoles. A existed period posaconazole 0.5237, 0.5 (0.25...
ABSTRACT We investigated the triazole, amphotericin B, and micafungin susceptibilities of 196 A. fumigatus clinical isolates in Nagasaki, Japan. The percentages non-wild-type (non-WT) for which MICs itraconazole, posaconazole, voriconazole were above ECV 7.1%, 2.6%, 4.1%, respectively. A G54 mutation cyp51A was detected 64.2% (9/14 isolates) 100% (5/5 non-WT itraconazole Amphotericin B ≥2 μg/ml minimum effective concentrations (MECs) ≥16 recorded two one isolates,
Proper protein folding in the endoplasmic reticulum (ER) is vital all eukaryotes. When misfolded proteins accumulate ER lumen, transmembrane kinase/endoribonuclease Ire1 initiates splicing of HAC1 mRNA to generate bZIP transcription factor Hac1, which subsequently activates its target genes increase protein-folding capacity ER. This cellular machinery, called unfolded response (UPR), believed be an evolutionarily conserved mechanism In this study, we comprehensively characterized mutant...
A Candida glabrata calcineurin mutant exhibited increased susceptibility to both azole antifungal and cell wall-damaging agents was also attenuated in virulence. Although a lacking the downstream transcription factor Crz1 displayed wall-associated phenotype intermediate that of modestly virulence, it did not show susceptibility. These results suggest regulates Crz1-dependent -independent pathways depending on type stress.
Although the diagnostic significance of isolating Aspergillus spp. from respiratory cultures has been studied in immunocompromised hosts with invasive pulmonary aspergillosis (IPA), little is known such infections immunocompetent patients other forms aspergillosis. In this study adult pneumology ward patients, we examined association between and disease prevalence. Laboratory records April 1998 to March 2009 were reviewed identify samples. Correlations isolated species clinical...
Diagnosing chronic pulmonary aspergillosis (CPA) is complicated, and there are limited data available regarding the identification of galactomannan (GM) in clinical specimens to assist detection this infection. The purpose study was evaluate GM bronchoalveolar lavage fluid (BALF) serum assess its utility for diagnosing CPA. We retrospectively reviewed diagnostic characteristics 144 patients, with without CPA, Nagasaki University Hospital, Japan, whose BAL were examined presence GM. Platelia...
ABSTRACT Streptococcus pneumoniae is the leading cause of respiratory infection worldwide. Although oral hygiene has been considered a risk factor for developing pneumonia, relationship between bacteria and pneumococcal unknown. In this study, we examined synergic effects Prevotella intermedia , major periodontopathic bacterium, on pneumonia. The supernatant P. (PiSup) pneumonia were investigated in mice, stimulation adhesion to human alveolar (A549) cells by PiSup was assessed....
Background Macrolides have antibiotic and immunomodulatory activities, which may a favorable effect on the clinical outcome of patients with infections, including influenza. This study aimed to evaluate effects combination therapy an anti-influenza agent, oseltamivir, single-dose formulation azithromycin (AZM), has been used for influenza-related secondary pneumonia, influenza patients. The primary endpoint was change in expression levels inflammatory cytokines. Secondary endpoints were time...
Abstract Long-term sequelae of the coronavirus disease (COVID-19) constitute Long COVID. Although COVID has been reported globally, its risk factors and effects on quality life (QOL) remain unclear. We conducted a cross-sectional study using questionnaires electronic medical records COVID-19 patients who were diagnosed or hospitalized at five facilities in Japan. Responses obtained from 285 out 1,150 patients. More than half participants symptoms varying severity 1 year after COVID-19....
In vitro drug screening studies have indicated that camostat mesilate (FOY-305) may prevent SARS-CoV-2 infection into human airway epithelial cells. This study was conducted to investigate whether is an effective treatment for (COVID-19).This a multicenter, double-blind, randomized, parallel-group, placebo-controlled study. Patients were enrolled if they admitted hospital within 5 days of onset COVID-19 symptoms or positive test asymptomatic patients. Severe cases (e.g., those requiring...
The dissemination of difficult-to-treat carbapenem-resistant Enterobacterales (CRE) is great concern. We clarified the risk factors underlying CRE infection mortality in Japan.We conducted a retrospective, multicentre, observational cohort study patients with infections at 28 university hospitals from September 2014 to December 2016, using Japanese National Surveillance criteria. Clinical information, including patient background, type infection, antibiotic treatment, and treatment outcome,...
Abstract Background Cross-neutralizing capacity of antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants is important in mitigating (re-)exposures. Role antibody maturation, the process whereby selection higher affinity augments host immunity, to determine SARS-CoV-2 neutralizing was investigated. Methods Sera from convalescents at 2, 6, or 10 months postrecovery, and BNT162b2 vaccine recipients 3 25 weeks postvaccination, were analyzed. Anti-spike IgG...
Background: Current influenza A vaccines primarily induce neutralizing antibodies targeting the variable hemagglutinin (HA) head domain, limiting their effectiveness against diverse or emerging virus (IAV) subtypes. The conserved HA stem particularly long α-helix (LAH) epitope, is a focus of universal vaccine research due to its cross-protective potential. Additionally, Fc-mediated functions such as antibody-dependent cellular cytotoxicity (ADCC) and phagocytosis (ADCP) are recognized...
Abstract Background The coronavirus disease 2019 (COVID-19) pandemic impacted infection control practices such as hand hygiene and antimicrobial use, but its specific effects remain unclear. Thus, in this multicenter study, we aimed to determine the pandemic's effect on these factors by comparing pre- post-COVID-19 periods. (A) number of inpatients month during study period. (B) amount sanitizer used per inpatient two solid lines show regression dashed line period is an extension...
Abstract Background Aspergillus fumigatus is a major fungal pathogen causing pulmonary aspergillosis, an important disease associated with transplantation and immunosuppression. However, the interactions between A. human cells are not well understood. This study evaluated interaction of THP-1 macrophages A549 lung epithelial using fluorescence imaging electrical impedance measurements to monitor growth cellular damage continuously.Figure 1.Schematic diagram relationship cell Methods The...
Background: Prescribing antiviral agents for severe acute respiratory syndrome coronavirus 2 requires careful consideration based on the patient’s risk factors disease progression and their vaccination status. However, effective interventions ensuring appropriate use of by physicians have yet to be fully established. Thus, this study evaluated impact an educational film prescription rates 2019 (COVID-19). Methods: This prospective, nationwide, web-based survey enrolled 1500 physicians. They...
Aim Elderly adults are at higher risk for severe COVID‐19 infection. This multicenter, prospective cohort study assessed immunogenicity after vaccinations in elderly residents compared with staff geriatric intermediate care facilities. Predictors of lower antibody titers were also examined. Methods Fifty‐four and 117 who had received three doses the mRNA vaccine between March 2021 September 2022 included. Anti‐receptor binding domain measured 3–4 weeks 6 months vaccinations. Adjusted...
We examined clinical (pulmonary cryptococcosis and cryptococcal meningitis) environmental (pigeon excreta) isolates of Cryptococcus neoformans var. (serotype A) in the southern Japanese prefecture Nagasaki. The random amplified polymorphic DNA profiles obtained by using three primers revealed six patterns among 21 8 isolates. Pattern I was most common (18 29 isolates) found throughout entire Nagasaki Prefecture. Patterns I, III, IV were both had a characteristic distribution, particular,...