A5065544054- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Immune Cell Function and Interaction
- HIV/AIDS Research and Interventions
- Heart Rate Variability and Autonomic Control
- Bacteriophages and microbial interactions
- Viral gastroenteritis research and epidemiology
- Influenza Virus Research Studies
- Cardiovascular and exercise physiology
- Virus-based gene therapy research
- RNA and protein synthesis mechanisms
- Cardiac Arrhythmias and Treatments
- Animal Disease Management and Epidemiology
- Protein Structure and Dynamics
- interferon and immune responses
- Cardiovascular Health and Disease Prevention
- Blood Pressure and Hypertension Studies
- RNA Interference and Gene Delivery
- Virology and Viral Diseases
- Respiratory viral infections research
- Animal Virus Infections Studies
- Hepatitis C virus research
- Atrial Fibrillation Management and Outcomes
- Monoclonal and Polyclonal Antibodies Research
- RNA Research and Splicing
National Institute of Infectious Diseases
2015-2024
Chiba University
2024
Omron (Japan)
2010-2023
Genomics (United Kingdom)
2009-2023
Tohoku Medical and Pharmaceutical University
2023
Northwestern University
2022
Critical Path Institute
2016-2019
Musashino Red Cross Hospital
2017
Tokyo Medical and Dental University
2017
Nagasaki University
2007-2014
Tissue culture infections of CD4-positive human T cells by immunodeficiency virus type 1 (HIV-1) proceed in three stages: (i) a period following the initiation an infection during which no detectable is produced; (ii) phase sharp increase followed peak released progeny virions can be measured; and (iii) final when production declines. In this study, we have derived equations describing kinetics HIV-1 accumulation cell supernatants multiple rounds infection. Our analyses indicated that...
<h3>Importance</h3> Mobile applications (apps) may help improve hypertension self-management. <h3>Objective</h3> To investigate the effect of an artificial intelligence smartphone coaching app to promote home monitoring and hypertension-related behaviors on systolic blood pressure level compared with a tracking app. <h3>Design, Setting, Participants</h3> This was 2-group, open, randomized clinical trial. Participants uncontrolled were recruited in 2016 2017 followed up for 6 months. Data...
Antiserum raised against a Fos peptide (amino acids Lys-127 to Arg-140 of chicken c-Fos) recognizes 46-kDa Fos-related protein in cell lysates growth-stimulated embryo fibroblasts. Induction the is transient but slightly prolonged relative c-Fos following growth stimulation. Using mixed oligonucleotide probe encoding antigen, we have cloned genomic locus that encodes this and determined its gene structure. This consists four exons, each which has some homology with corresponding exons c-fos...
The balance of effector and regulatory T cell function, dependent on multiple signals epigenetic regulators, is critical to immune self-tolerance. Dysregulation helper 17 (Th17) cells associated with autoimmune diseases, including sclerosis. Here, we report that Sirtuin 1 (SIRT1), a protein deacetylase previously reported have an antiinflammatory in fact promotes autoimmunity by deacetylating RORγt, the signature transcription factor Th17 cells. SIRT1 increases RORγt transcriptional...
For high-intensity cycle ergometer exercise, the relation between power (P) and its tolerable duration (t) has been well characterized by hyperbolic relationship: (P-θF)t = W', or P W'(1/t)+θF, where θF may be termed ‘fatigue threshold’. The curvature constant (W') reflects a amount of work which is postulated to equivalent finite energy store that relates oxygen-deficit: phosphagen pool, anaerobic glycolysis oxygen stores. Compared thetaF, physiological nature W' received little...
ABSTRACT Norovirus GII/4 is a leading cause of acute viral gastroenteritis in humans. We examined here how the virus evolves to generate and sustain new epidemics humans, using 199 near-full-length genome sequences 11 segment clones from human stool specimens collected at 19 sites Japan between May 2006 February 2009. Phylogenetic studies demonstrated outbreaks 7 monophyletic subtypes, among which single subtype, termed 2006b, had continually predominated. Phylogenetic-tree,...
Between September 2013 and July 2014, 2,482 influenza 2009 pandemic A(H1N1) [A(H1N1)pdm09] viruses were screened in Japan for the H275Y substitution their neuraminidase (NA) protein, which confers cross-resistance to oseltamivir peramivir. We found that a large cluster of mutant virus was present prior main season Sapporo /: Hokkaido, with detection rate this reaching 29% area. Phylogenetic analysis suggested clonal expansion single Hokkaido. To understand reason cluster, we examined vitro...
The human papillomavirus type 16 E7 gene encodes a nuclear oncoprotein (98 amino acids [AAs] long) consisting of three regions: regions 1 (AAs to 20) and 2 21 40), which show high homology the sequences conserved domains 2, respectively, adenovirus E1A; region 3 41 98) containing two metal-binding motifs Cys-X-X-Cys 58 91 94). We constructed AA deletion (substitution) mutants single-AA substitution placed under control simian virus 40 promoter examined their biological functions. Stable...
Our norovirus (NoV) surveillance group reported a >4-fold increase in NoV infection Japan during the winter of 2006-2007 compared to previous winter. Because was not linked changes system, we suspected emergence new GII/4 epidemic variants. To obtain information on viral changes, conducted full-length genomic analysis. Stool specimens from 55 acute gastroenteritis patients various ages were collected at 11 sites between May 2006 and January 2007. Direct sequencing long PCR products revealed...
ABSTRACT Tetherin, also known as BST-2/CD317/HM1.24, is an antiviral cellular protein that inhibits the release of HIV-1 particles from infected cells. viral U (Vpu) a specific antagonist human tetherin might contribute to high virulence HIV-1. In this study, we show three amino acid residues (I34, L37, and L41) in transmembrane (TM) domain are critical for interaction with Vpu by using live cell-based assay. We found conservation additional at position 45 two downstream 22, which absent...
Virus replication in the host proceeds by chains of interactions between viral and proteins. The are deeply influenced immune molecules anti-viral compounds, as well mutations To understand how these proceed mechanically they mutations, one needs to know structures dynamics Molecular (MD) simulation is a powerful computational method for delineating motions proteins at an atomic-scale via theoretical empirical principles physical chemistry. Recent advances hardware software biomolecular have...
ABSTRACT The emergence of syncytium-inducing (SI) variants human immunodeficiency virus type 1 (HIV-1) in infected individuals is an indicator poor prognosis and often correlated with faster CD4 + cell depletion rapid disease progression. Interleukin-4 (IL-4) a pleiotropic cytokine various immune-modulating functions including induction immunoglobulin E (IgE) production B cells, down-regulation CCR5 (a coreceptor for HIV-1 non-SI [NSI] strains), up-regulation CXCR4 SI variants). Here we show...
For high-intensity cycle ergometer exercise, the tolerable duration (t) is well characterized as a hyperbolic function of power output, P : t = W'/(P-thetaF), where thetaF may be termed "fatigue threshold." The purpose this study was to determine effect oral creatine (Cr) supplementation on curvature constant parameter (W') power-duration curve. A double-blind research method and cross-over design were employed for creatine/placebo supplementation. Eight healthy male subjects (aged 18 22...
ABSTRACT Human immunodeficiency virus type 2 (HIV-2) strains vary widely in their abilities to grow Old World monkey (OWM) cells such as those of cynomolgus monkeys (CM). We evaluated eight HIV-2 isolates for sensitivities CM TRIM5α, an anti-HIV factor OWM cells. found that different showed differences TRIM5α. Sequence analysis TRIM5α-sensitive viruses had proline at the 120th position capsid protein (CA), whereas TRIM5α-resistant either alanine or glutamine. Mutagenesis studies indicated...
Middle East respiratory syndrome coronavirus (MERS-CoV) infection can manifest as a mild illness, acute distress, organ failure, or death. Several animal models have been established to study disease pathogenesis and develop vaccines therapeutic agents. Here, we developed transgenic (Tg) mice on C57BL/6 background; these expressed human CD26/dipeptidyl peptidase 4 (hDPP4), functional receptor for MERS-CoV, under the control of an endogenous hDPP4 promoter. We then characterized this mouse...
Now that highly active antiretroviral therapy (HAART) is being initiated on a large scale in West Africa, it remains controversial whether protease inhibitors (PIs), originally designed and tested against human immunodeficiency virus type 1 (HIV-1) subtype B, are equally effective the non-B subtypes prevalent African countries. In this study, we investigated Ghanaian HIV-1 isolates, as representatives of susceptible to PIs.We first generated an cassette vector proviral DNA carrying...
Etravirine (ETV) is a second-generation nonnucleoside reverse transcriptase (RT) inhibitor (NNRTI) introduced recently for salvage antiretroviral treatment after the emergence of NNRTI-resistant human immunodeficiency virus type 1 (HIV-1). Following its introduction, two naturally occurring mutations in HIV-1 RT, V106I and V179D, were listed as ETV resistance-associated mutations. However, effect these on development NNRTI resistance has not been analyzed yet. To select highly vitro,...
Background The net charge of the hypervariable V3 loop on HIV-1 envelope gp120 outer domain plays a key role in modulating viral phenotype. However, molecular mechanisms underlying modulation remain poorly understood. Methodology/Principal Findings By combining computational and experimental approaches, we examined how could influence phenotype interaction surface. Molecular dynamics simulations identical domain, carrying with +3 or +7, showed that change alone induce global changes...