Additive manufacturing (AM) is gaining interests in drug delivery applications, offering innovative opportunities for the design and development of systems with complex geometry programmed controlled release profile. In addition, polymer-based can improve safety, efficacy, patient compliance, are key materials AM. Therefore, combining AM polymers be beneficial to overcome existing limitations systems. Considering these advantages, here we focusing on recent developments field polymeric...

Mesenchymal stromal cells (MSCs) are an attractive therapeutic agent in regenerative medicine. Recently, there has been a paradigm shift from differentiation of MSCs to their paracrine effects at the injury site. Several reports elucidate role trophic factors secreted by toward repair injured tissues. We hypothesize that fractionating MSC secretome will enrich exosomes containing soluble bioactive molecules, improving its potential for liver failure.Rat bone marrow were isolated and...

The aim of this study was to synthesize and characterize a nano-hydroxyapatite (nHAP) calcium sulfate bone substitute (NC) for cranioplasty. NC functionalized with low concentrations morphogenetic protein-2 (BMP-2) zoledronic acid (ZA) characterized both in vitro vivo. In studies included MTT, ALP assays, fluorescent staining Saos-2 (human osteoblasts) MC3T3-E1 (murine preosteoblasts) cells cultured on NC. An vivo divided 20 male Wistar rats into four groups: control (defect only), NC, + ZA,...

Cationic polysaccharides have demonstrated significant antimicrobial properties and great potential in medical applications, where the antiviral activity is of interest. As today, alcohols oxidizing agents are commonly used as disinfectants. However, these compounds not environmentally safe, short periods, may cause health issues. Therefore, this study aimed to develop metal-free friendly quaternary chitosans (QCs) with excellent long-lasting virucidal activity. To evaluate this, both single...

The demand for new biocompatible and 3D printable materials biomedical applications is on the rise. Ideally, such should exhibit either biodegradability or recyclability, possess antibacterial properties, demonstrate remarkable biocompatibility with no cytotoxic effects. In this research, we synthesized hydrogels tailored applications, as wound healing films, by combining double-quaternized chitosan (DQC) cystamine-based non-isocyanate polyurethane (NIPU-Cys) - a material renowned enhancing...

Repair and regeneration of critically injured peripheral nerves is one the most challenging reconstructive surgeries. Currently available FDA approved nerve guidance channels (NGCs) are suitable for small gap injuries, their biological performance inferior to that autografts. Development biomimetic NGCs with clinically relevant geometrical characteristics such as topographical, biochemical, haptotactic cues could offer better long-gap complex injuries. Here, in this study, we present...

Silk cocoon membrane (SCM) is an insect engineered structure. We studied the electrical properties of mulberry (Bombyx mori) and non-mulberry (Tussar, Antheraeamylitta) SCM. When dry, SCM behaves like insulator. On absorbing moisture, it generates current, which modulated by temperature. The current flowing across possibly ionic protonic in nature. exploited to develop simple energy harvesting devices, could operate low power electronic systems. Based on our findings, we propose that...

Recent progress in the use of decellularized organ scaffolds as regenerative matrices for tissue engineering holds great promise addressing issue donor shortage. Decellularization preserves mechanical integrity, composition, and microvasculature critical zonation hepatocytes liver. Earlier studies have reported possibility repopulating with hepatic cell lines or stem cells to improve liver regeneration. In this work, we study versatility matrix a substrate coating three-dimensional cryogel...

Diabetic wounds are instantaneously prone to the bacterial infections that can delay healing process. An efficient wound dressing material is critical a fast of in diabetic patients. The present study focuses on synthesis yeast extract (YE)-immobilized and copper (Cu) nanoparticle (NP)-dispersed carbon nanofibers (CNFs) as potential material. biological assays, namely, platelet aggregation, hemolysis, cells viability, proliferation macrophage show prepared biomaterial be noncytotoxic....

In this work, we have synthesized injectable bone cement incorporated with gelatin to enhance cellular interaction. Human osteosarcoma Saos-2 cells derived morphogenetic proteins (BMP's) and a bisphosphonate (zoledronic acid (0.2 mM)) were also cement. vitro studies conducted using demonstrated enhanced cell proliferation on (0.2%w/v) The differentiation of C2C12 mouse myoblast into forming showed 6-fold increase in ALP levels Polymerase chain reaction (PCR) for biomarkers osteoinductive...

Hip fractures are among the most common types of fracture risks in old age osteoporotic patients that often end up with immobile disabilities. Weak bones due to loss mineral content along an increase porosity femur neck canal osteoporosis reduce mechanical properties bone and predispose fractures. In this study, we have used calcium sulfate/nanohydroxyapatite based nanocement (NC) as carrier recombinant human morphogenetic protein-2 (BMP-2), zoledronate (ZA), marrow mesenchymal stromal cells...

Large critical size bone defects are complicated to treat, and in many cases, autografts become a challenge due availability. In such situations, synthetic implant that can be patient-specifically designed fabricated with control over parameters as porosity, rigidity, osteogenic cues act potential substitute. this study, we produced photocuring composite resins poly(trimethylene carbonate) containing high ratios of bioactive ceramics printed porous 3D scaffolds used grafts. To enhance the...

The periosteum is an indispensable part of the bone that nourishes cortical and acts as a repertoire osteoprogenitor cells. Periosteal damage result traumatic injuries, infections, or surgical assistance in surgeries often associated with high incidence delayed healing (union nonunion) compounded severe pain risk secondary fracture. Developing bioengineered functional periosteal substitutes approach to augment healing. In this study, we have developed biomimetic membrane consisting...

Next-generation treatment strategies to treat osteomyelitis with complete eradication of pathogen at the bone nidus and prevention emergence drug resistance is a real challenge in orthopedics. Conventional including long-term adherence patients systemic antibiotic delivery, local delivery using nondegradable vehicles, surgical debridement are not completely effective achieving successful results. In this study, broad-spectrum antibiotic, rifampicin (RFP), was incorporated into biphasic...

Cationically modified chitosan derivatives exhibit a range of appealing characteristics, with particular emphasis on their antimicrobial potential across broad spectrum biomedical applications.

3D printing has progressed significantly, allowing objects to be produced using a wide variety of materials. Recent advances have employed focused ultrasound in printing, allow inside acoustically transparent Here we introduce Selective Ultrasonic Melting (SUM) method for poly ({\epsilon}-caprolactone) (PCL) powder mixed with water. The was done by mechanically moving transducer. microstructure and porosity the prints were analyzed micro-computed tomography ({\mu}CT). open printed samples...

In the present work, development of high strength biocompatible polyvinyl alcohol (PVA) based composite films using citric acid (CA) as a crosslinker and bacterial cellulose nanowhiskers (BCNW) nanofiller is reported. The interfacial binding between nanoreinforcement polymer modified by covalently them acid. These demonstrate highest tensile 132 MPa in comparison to PVA (37 MPa). also shows 10‐fold reduction water swelling percentage better thermal properties compared PVA. Hemocompatibility...

ABSTRACT The present study describes microwave (MW)‐assisted rapid synthesis of biocompatible poly(vinyl alcohol) (PVA) composite films that demonstrate synergy between reinforcement and crosslinking. Bacterial cellulose (5% w/w) nanowhiskers (reinforcement) tartaric acid 35% (w/w) (crosslinker) are incorporated in PVA to prepare crosslinked cellulose–PVA films. properties thus prepared compared with samples conventional hot air oven heating (CH). Crosslinking by both the methods reduces...

Abstract Long term multiple systemic antibiotics form the cornerstone in treatment of bone and joint tuberculosis, often combined with local surgical eradication. Implanted carriers for drug delivery have recently been introduced to overcome some limitations associated conventional strategies. In this study, we used a calcium sulphate hemihydrate (CSH)/nanohydroxyapatite (nHAP) based nanocement (NC) biomaterial as void filler well carrier two standard care tuberculosis drugs, Rifampicin...

With the advances in tissue engineering and regenerative medicine, various approaches have been developed for peripheral nerve repair regeneration. In current study, we synthesized a cryogel matrix from chitosan gelatin incorporated with polypyrrole neural The three-dimensional (3-D) was fabricated to mimic vivo microenvironment analyzed stem cell differentiation. Isolated bone marrow cells (BMSCs) cultured on 3-D differentiated into lineage basis of scaffold properties, co-culture system by...

Ceramic biomaterials are promising alternatives to bone autografts. However, limited bioactivity affects their performance. Therefore, bioactive molecules and cells often added enhance Exosomes have emerged as cell-secreted vesicles, delivering proteins, lipids, nucleic acids in a paracrine/endocrine fashion. We studied two complementary aspects required for exosome activity/therapy using purified exosomes: first, the intracellular uptake of labeled exosomes second, influence delivered on...

Polymer (acrylate) and ceramic bone cements are extensively used as void fillers for implant fixation in orthopedics. These materials have micro- to nonporous architectures. Postimplantation, they may cause hypoxic exothermic injuries already compromised damage site. also limited interaction with surrounding tissue. In this work we developed composite collagen-nanohydroxyapatite (CS) filler, mimicking porous architecture of trabecular bone. It was functionalized clinically available active...