A5001629306- Viral Infectious Diseases and Gene Expression in Insects
- Monoclonal and Polyclonal Antibodies Research
- Protein purification and stability
- CRISPR and Genetic Engineering
- Microbial Metabolic Engineering and Bioproduction
- Virus-based gene therapy research
- Glycosylation and Glycoproteins Research
- Liver physiology and pathology
- Pancreatic function and diabetes
- Transgenic Plants and Applications
- Enzyme Catalysis and Immobilization
- Animal Genetics and Reproduction
- Clinical Nutrition and Gastroenterology
- Biofuel production and bioconversion
- Organ Transplantation Techniques and Outcomes
- RNA Interference and Gene Delivery
- Endoplasmic Reticulum Stress and Disease
- CAR-T cell therapy research
- Animal Virus Infections Studies
- 3D Printing in Biomedical Research
- Advanced Biosensing Techniques and Applications
- Bacterial Genetics and Biotechnology
- Biopolymer Synthesis and Applications
- Amino Acid Enzymes and Metabolism
- RNA and protein synthesis mechanisms
Osaka University
2016-2025
KM Biologics (Japan)
2024
Cell Biotech (South Korea)
2023
Biocat
2023
US Biologic (United States)
2016-2022
Tokushima University
2011-2020
Institute of Engineering Science
2014
National Center For Child Health and Development
2012
Tohoku University
2003
Suita Municipal Hospital
2003
Abstract To investigate the effects of lactate on cell growth and antibody production, a new method maintaining concentration constant in fed‐batch culture is described. When pH was initially adjusted by sodium hydroxide, specific rate decreased death increased with an increase concentration. whether inhibition due to itself or osmotic pressure, effect pressure chloride compared that lactate. same condition as using chloride, data showed degree inhibition. It thus evident mainly while...
Abstract Background The integration of biotechnology into chemical manufacturing has been recognized as a key technology to build sustainable society. However, the practical applications biocatalytic conversions are often restricted due their complexities involving unpredictability product yield and troublesome controls in fermentation processes. One possible strategies overcome these limitations is eliminate use living microorganisms only enzymes involved metabolic pathway. Use recombinant...
Abstract To improve the production of recombinant human antithrombin III (AT‐III) in Chinese hamster ovary (CHO) cells, genes encoding transcription factors, ATF4 (activating factor 4) and XBP‐1s (the spliced form X‐box binding protein 1), which were involved mammalian unfolded response (UPR), cloned from CHO‐K1 cells. Overexpression significantly enhanced AT‐III CHO 13D‐35D The specific rate ATF4‐overexpressed cells reached approximately 23 pg/cell/day. After 144 h incubation, concentration...
Chinese hamster ovary (CHO) cells have frequently been used in biotechnology for many years as a mammalian host cell platform cloning and expressing genes of interest. A detailed physical chromosomal map the CHO DG44 line was constructed by fluorescence situ hybridization (FISH) imaging using randomly selected 303 BAC clones probes (BAC-FISH). The two longest chromosomes were completely paired chromosomes; other partly deleted or rearranged. end sequences 624 clones, including 287 mapped...
Abstract Most cells active in the immune system express receptors for antibodies which mediate a variety of defensive mechanisms. These interact with Fc portion antibody and are therefore collectively called receptors. Here, using high-speed atomic force microscopy, we observe interactions human, humanized, mouse/human-chimeric immunoglobulin G1 (IgG1) their cognate receptor, FcγRIIIa. Our results demonstrate that not only but also Fab positively contributes to interaction receptor....
Studies of recombinant adeno-associated virus (rAAV) revealed the mixture full particles with different densities in rAAV. There are no conclusive results because lack quantitative stoichiometric viral proteins, encapsidated DNA, and particle level analyses. We report first comprehensive characterization low- high-density rAAV serotype 2 particles. Capillary gel electrophoresis showed possessing a designed DNA capsid composed (VP1 + VP2)/VP3 = 0.27, whereas low-density have same but...
Genomics-based safety and quality evaluation studies are advancing the bioindustry by enhancing various aspects, including viral safety, host cell protein (HCP) control, product heterogeneity cellular process reproducibility. High-throughput instruments genome-scale databases essential in genomics, with reference genome sequence being most critical database. The completeness accuracy of these sequences depend on DNA quality, sequencing instruments, read coverage, assembly strategies....
Previously, we established an easy and quick construction method for obtaining a stable highly productive gene-amplified recombinant Chinese hamster ovary (CHO) cell line. With gradual increase in methotrexate (MTX) concentration, pools had high specific growth production rates. Moreover, the phenotype of cells seemed to be affected by location amplified gene chromosomal DNA. We suspected that various kinds might appear during long-term selection construct pools. To clarify behavior stepwise...
The purpose of this study was to evaluate and compare the osteoblastic differentiation ability dedifferentiated fat (DFAT) cells adipose stem (ASCs) from buccal pad (BFP).We isolated human DFAT ASCs BFP a patient who underwent oral maxillofacial surgery then analyzed their cell surface antigens by flow cytometry. Then, were cultured in osteogenic medium for 14 days. Measurement bone-specific alkaline phosphatase (BAP), osteocalcin (OCN), calcium deposition alizarin red staining performed...
Abstract The NfxC‐type mutant of Pseudomonas aeruginosa produces the MexEF‐OprN efflux pump and down‐regulates expression quorum‐sensing‐dependent MexAB‐OprM production virulence factors in presence an active transcriptional regulator, MexT. Consequently, these cells are resistant to chloramphenicol hypersusceptible β‐lactam antibiotics. An upper negative MexS, has been assumed inactivate MexT wild‐type strains, hence shutting down pump. This observation was, however, reported only one...