A5006579357- Viral Infectious Diseases and Gene Expression in Insects
- Monoclonal and Polyclonal Antibodies Research
- Protein purification and stability
- Pluripotent Stem Cells Research
- Transgenic Plants and Applications
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- Hemoglobinopathies and Related Disorders
- CAR-T cell therapy research
- Nitric Oxide and Endothelin Effects
- Eicosanoids and Hypertension Pharmacology
- Renal and related cancers
- Iron Metabolism and Disorders
- Animal Genetics and Reproduction
- Renin-Angiotensin System Studies
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Glycosylation and Glycoproteins Research
- Viral Infections and Immunology Research
- Reproductive Biology and Fertility
- Adipokines, Inflammation, and Metabolic Diseases
- Animal Virus Infections Studies
- Cancer, Hypoxia, and Metabolism
- Education in Diverse Contexts
- Heat shock proteins research
- Fibroblast Growth Factor Research
Osaka University
2008-2024
KM Biologics (Japan)
2024
Cell Biotech (South Korea)
2023
Biocat
2023
Tokushima University
2011-2016
Okayama University of Science
2006-2007
Iron is an essential trace metal for most organisms. However, excess iron causes oxidative stress through production of highly toxic hydroxyl radicals via the Fenton/Haber-Weiss reaction. storage in body reported to be associated with fat accumulation and type 2 diabetes mellitus. We investigated role adiposity by using KKAy mice obese diabetic model mice. Eight-week-old were divided into two groups treated deferoxamine (DFO), chelator agent, or a vehicle wk. DFO treatment diminished...
Primordial germ cells (PGCs) are embryonic cell precursors. Although the developmental potency of PGCs is restricted to lineage, can acquire pluripotency, as verified by in vitro establishment (EG) and vivo production testicular teratomas. PGC-specific inactivation PTEN, which a lipid phosphatase antagonizing phosphoinositide-3 kinase (PI3K), enhances both EG teratoma formation. Here, we analyzed effect serine/threonine AKT, one major downstream effectors PI3K, on PGCs. We used transgenic...
Vascular remodelling is mediated by vascular smooth muscle cell (VSMC) proliferation and hypertrophy, both processes of which are linked to medial thickening fibrosis. Here, we show that hypoxia-inducible factor-1α (Hif-1α) expressed in cells (SMCs) involved angiotensin II (Ang II)-induced an vivo model. To clarify the role Hif-1α remodelling, created mice lacking gene SMCs (SMKO mice). Ang infusion induced fibrosis, accompanied up-regulation, aortae control mice, but not those SMKO mice. In...
It is known that obese adipose tissues are hypoxic and express hypoxia-inducible factor (HIF)-1α. Although some studies have shown the expression of HIF-1α in adipocytes induces glucose intolerance, mechanisms still not clear. In this study, we examined its effects on development type 2 diabetes by using adipocyte-specific knockout (ahKO) mice. ahKO mice showed improved tolerance compared with wild (WT) Macrophage infiltration mRNA levels monocyte chemotactic protein-1 (MCP-1) tumor necrosis...
Studies of recombinant adeno-associated virus (rAAV) revealed the mixture full particles with different densities in rAAV. There are no conclusive results because lack quantitative stoichiometric viral proteins, encapsidated DNA, and particle level analyses. We report first comprehensive characterization low- high-density rAAV serotype 2 particles. Capillary gel electrophoresis showed possessing a designed DNA capsid composed (VP1 + VP2)/VP3 = 0.27, whereas low-density have same but...
Genomics-based safety and quality evaluation studies are advancing the bioindustry by enhancing various aspects, including viral safety, host cell protein (HCP) control, product heterogeneity cellular process reproducibility. High-throughput instruments genome-scale databases essential in genomics, with reference genome sequence being most critical database. The completeness accuracy of these sequences depend on DNA quality, sequencing instruments, read coverage, assembly strategies....
Primordial germ cells (PGCs) are embryonic cell precursors. Specification of PGCs occurs under the influence mesodermal induction signaling during in vivo gastrulation. Although bone morphogenetic proteins and Wnt play pivotal roles both PGC specification, signal regulating specification remains unknown. Coculture mouse stem (ESCs) with OP9 feeder induces differentiation vitro. Using this system, we demonstrated that PGC-like were efficiently induced from ESCs by extracellular...
Although iron is an essential trace metal, its presence in excess causes oxidative stress the human body. Recent studies have indicated that storage a risk factor for type 2 diabetes mellitus. Dietary restriction or chelation ameliorates symptoms of mouse models. However, whether content body changes with development unknown. Here, we investigated dynamics accumulation and absorption-related genes mice developed obesity by consuming high-fat diet (HFD-fed mice). HFD-fed (18-20 wk) were...
Abstract Tissue kallikrein mK1 is a serine protease involved in the generation of bioactive kinins for normal cardiac and arterial function mouse. In present study, tissue gene Klk1 , which codes mK1, was shown to be one most prevalent Klk species uteri adult mice, its mRNA level significantly higher at estrus than diestrus. expression enhanced ovariectomized mice receiving estradiol‐17β treatment. Both endometrial epithelial stromal cells isolated from exhibited detectable levels when...
Bioprocess development benefits from kinetic models in many aspects, including scale-up, optimization, and process understanding. However, current are unable to simulate the production of a coxsackievirus A6 (CVA6) virus-like particle (VLP) vaccine using Chinese hamster ovary cell culture. In this study, novel model was constructed, correlating (1) growth, death, lysis kinetics, (2) metabolism major metabolites, (3) CVA6 VLP production. To construct model, two batches laboratory-scale 2 L...
Abstract Chinese hamster ( Cricetulus griseus ) ovary-derived ovary (CHO) cells are the most commonly used mammalian hosts for industrial production of recombinant therapeutics because their ability to fold, assemble, and perform post-translational modifications, such as glycosylation, on proteins. They also valuable grow in serum-free suspension cultures. In this study, we established a cell line derived from lung tissue hamsters, named (CHL)-YN cells. The biosafety CHL-YN was confirmed by...
Diabetic nephropathy (DN) is the major cause of end-stage renal failure. Oxidative stress implicated in pathogenesis DN. Nitrosonifedipine (NO-NIF) a weak calcium channel blocker that converted from nifedipine under light exposure. Recently, we reported NO-NIF has potential as novel antioxidant with radical scavenging abilities and capacity to treat vascular dysfunction by exerting an endothelial protective effect. In present study, extended these findings evaluating efficacy against DN...
Background Therapeutic antibodies have become an important focus of the biopharmaceutical industry. The Chinese hamster ovary (CHO) cell line is a major host for therapeutic antibody production. To construct productive CHO lines, two transfection methods are commonly used, i.e., random integration and gene targeting. Random common method in which randomly integrated transgenes cause variation productivity because they located various chromosomal regions that affect transgene expression...