A5052962043- Epigenetics and DNA Methylation
- Genetic Syndromes and Imprinting
- Pluripotent Stem Cells Research
- Renal and related cancers
- Pancreatic function and diabetes
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- Chemical Reactions and Isotopes
- Prenatal Screening and Diagnostics
- Pancreatic and Hepatic Oncology Research
- Reproductive Biology and Fertility
- Growth Hormone and Insulin-like Growth Factors
- Animal Genetics and Reproduction
- Birth, Development, and Health
- DNA Repair Mechanisms
- Cancer-related molecular mechanisms research
- Genetic diversity and population structure
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genomic variations and chromosomal abnormalities
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Genetics and Neurodevelopmental Disorders
- Congenital heart defects research
- Cardiovascular Disease and Adiposity
- Amphibian and Reptile Biology
- Cardiomyopathy and Myosin Studies
Kitasato University
2014-2024
University of Cambridge
2009-2024
National Institute for Health Research
2022-2024
Medical Research Council
2024
NIHR Cambridge Biomedical Research Centre
2022
Wellcome/MRC Institute of Metabolic Science
2019-2022
Wellcome Trust
2022
Addenbrooke's Hospital
2019-2021
Cell Biotech (South Korea)
2019
Nagahama Institute of Bio-Science and Technology
2016
We present a draft genome sequence of the platypus, Ornithorhynchus anatinus. This monotreme exhibits fascinating combination reptilian and mammalian characters. For example, platypuses have coat fur adapted to an aquatic lifestyle; platypus females lactate, yet lay eggs; males are equipped with venom similar that reptiles. Analysis first aligned these features genetic innovations. find reptile proteins been co-opted independently from same gene families; milk protein genes conserved despite...
Undernutrition during pregnancy reduces birth weight and programmes adult phenotype with consequences for life expectancy, but its effects on the of placenta, responsible supplying nutrients fetal growth, remain largely unknown. Using molecular, morphological functional analyses, placental was examined in mice restriction dietary intake to 80% control from day 3 pregnancy. At 16, undernutrition reduced placental, not fetal, association decreased junctional zone volume expression glucose...
Primordial germ cells (PGCs) are embryonic cell precursors. Specification of PGCs occurs under the influence mesodermal induction signaling during in vivo gastrulation. Although bone morphogenetic proteins and Wnt play pivotal roles both PGC specification, signal regulating specification remains unknown. Coculture mouse stem (ESCs) with OP9 feeder induces differentiation vitro. Using this system, we demonstrated that PGC-like were efficiently induced from ESCs by extracellular...
Abstract The Dlk1-Dio3 imprinted domain is controlled by an imprinting control region (ICR) called IG-DMR that hypomethylated on the maternal allele and hypermethylated paternal allele. Although several genetic mutation experiments have shown essential for of domain, how DNA methylation itself functions has not been elucidated. Here, we performed both gain loss targeting transiently introducing CRISPR/Cas9 based-targeted editing tools along with one guide RNA into mouse ES cells. Altered...
Although recent studies in patients with paternal uniparental disomy 14 [upd(14)pat] and other conditions affecting the chromosome 14q32.2 imprinted region have successfully identified underlying epigenetic factors involved development of upd(14)pat phenotype, several matters, including regulatory mechanism(s) for RTL1 expression, imprinting status DIO3 placental histological characteristics, remain to be elucidated. We therefore performed molecular using fresh samples from two upd(14)pat....
Abstract Mouse parthenogenetic haploid embryonic stem cells (ESCs) are pluripotent generated from chemically activated oocytes. Haploid ESCs provide an opportunity to study the effect of genetic alterations because their hemizygotic characteristics. However, further application for selection unique phenotypes remains limited since ideal reporters monitor biological processes such as cell differentiation missing. Here, we report CRISPR/Cas9-mediated knock-in a reporter cassette, which does...
Abstract Primordial germ cells (PGCs) can give rise to pluripotent stem known as embryonic (EGCs) when cultured with basic fibroblast growth factor (bFGF), cell (SCF), and leukemia inhibitory factor. Somatic induced (iPSCs) by introduction of the reprogramming transcription factors Oct4, Sox2, Klf4. The effects Sox2 Klf4 on somatic be reproduced using small molecule compounds, transforming factor-β receptor (TGFβR) inhibitor Kempaullone, respectively. Here we examined TGFβR Kempaullone EGC...
Abstract Spermatogenesis is a reproductive system process that produces sperm. Ubiquitin specific peptidase 26 (USP26) an X chromosome-linked deubiquitinase specifically expressed in the testes. It has long been controversial whether USP26 variants are associated with human male infertility. Thus, present study, we introduced mutation into Usp26 gene mice and found mutant males backcrossed to DBA/2 background, but not C57BL/6 were sterile or subfertile had atrophic These findings indicate...
The imprinted region on mouse distal chromosome 12 covers about 1 Mb and contains at least three paternally expressed genes <i>(Pegs</i>: <i>Peg9/Dlk1</i>, <i>Peg11/Rtl1</i>, <i>Dio3) </i>and four maternally <i>(Megs</i>: <i>Meg3/Gtl2</i>, <i>antiPeg11/antiRlt1</i>, <i>Meg8/Rian</i>, and<i> Meg9/</i><i>Mirg)</i>. <i>Gtl2</i><sup>lacZ</sup> (Gene trap...
The genetic mechanisms that determine the size of adult pancreas are poorly understood. Imprinted genes, which expressed in a parent-of-origin-specific manner, known to have important roles development, growth and metabolism. However, our knowledge regarding their control pancreatic function remains limited. Here we show many imprinted genes highly mesenchyme-derived cells explore role paternally-expressed insulin-like factor 2 ( Igf2 ) gene mesenchymal epithelial lineages using newly...
Abstract Sexual reproduction involves the creation of sex-dependent gametes, oocytes and sperm. In mammals, sexually dimorphic differentiation commences in primordial germ cells (PGCs) embryonic gonads; PGCs ovaries testes differentiate into meiotic primary mitotically quiescent prospermatogonia, respectively. Here, we show that transition from to sex-specific was abrogated conditional knockout mice carrying a null mutation Smarcb1 (also known as Snf5 ) gene, which encodes core subunit...
Nucleotide-binding oligomerization domain, leucine-rich repeat and pyrin domain-containing proteins (NRLPs) are central components of the inflammasome. Accumulating evidence has shown that a reproductive clade NRLPs is predominantly expressed in oocyte to cleavage stage embryos participates mammalian preimplantation development as component multiprotein complex known subcortical maternal (SCMC). Nlrp9s belong class NLRPs; Nlrp9b unique acting an inflammasome against rotavirus intestines....
Abstract Background Phosphoinositide-3 kinase (PI3K)/AKT signaling participates in cellular proliferation, survival and tumorigenesis. The activation of AKT promotes the reprogramming including generation induced pluripotent stem cells (iPSCs) dedifferentiation primordial germ (PGCs). Previous studies suggested that by activating proliferation glycolysis. Here we report a line evidence supports notion is involved TET-mediated DNA demethylation during iPSC induction. Methods was activated...