A5101521528- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Molecular Biology Techniques and Applications
- Epigenetics and DNA Methylation
- Renal and related cancers
- RNA and protein synthesis mechanisms
- Chemical Reactions and Isotopes
- Machine Learning in Bioinformatics
- Enzyme Structure and Function
- RNA Research and Splicing
- Pluripotent Stem Cells Research
- Glycosylation and Glycoproteins Research
- Oral microbiology and periodontitis research
- S100 Proteins and Annexins
- Receptor Mechanisms and Signaling
- Ubiquitin and proteasome pathways
- HIV Research and Treatment
- Platelet Disorders and Treatments
- Biosensors and Analytical Detection
- Neuroendocrine Tumor Research Advances
- Galectins and Cancer Biology
- Protein purification and stability
- Monoclonal and Polyclonal Antibodies Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
Kitasato University
2016-2025
Chiba University Hospital
2006-2014
Institute of Bioinformatics
2013
Kitasato University Hospital
2012
Chiba University
2010
National Hospital Organization
2010
Fukuoka Women's University
2007
Mitsubishi Group (Japan)
1999
RIKEN
1999
Tokyo Medical and Dental University
1999
Abstract Purpose: Although numerous proteome studies have been performed recently to identify cancer-related changes in protein expression, only a limited display of relatively abundant proteins has identified. The aim this study is novel as potential tumor markers primary colorectal cancer tissues using high-resolution two-dimensional gel electrophoresis (2-DE). Experimental Design: 2-DE an agarose for isoelectric focusing was used compare profiling 10 and adjacent normal mucosa. Altered...
Protein phosphorylation is a key mechanism of cellular signaling pathways and aberrant has been implicated in number human diseases. Thus, approaches phosphoproteomics can contribute to the identification biomarkers assess disease pathogenesis drug targets. Moreover, careful validation large-scale phosphoproteome analysis, which lacking current protein-based biomarker discovery, significantly increases value identified biomarkers. Here, we performed differential analysis using IMAC coupled...
Serum proteins/peptides reflect physiological or pathological states in humans and are an attractive target for the discovery of disease biomarkers. However, existence high-abundance proteins large dynamic range serum make any quantitative analysis low-abundance challenging. Furthermore, analyses peptides, including cleaved fragments proteins, difficult because carrier protein binding. Here, we developed a differential solubilization (DS) method to extract low-molecular-weight with good...
Pancreatic ductal adenocarcinoma (PDAC) remains a devastating disease due to the lack of specific early diagnostic markers. To improve outcomes, proteomic approaches are being developed for discovery novel biomarkers PDAC.Using tandem mass tag labelling and LC-MS/MS, we performed comparative analyses pre- postoperative sera from PDAC patients identify serum PDAC. In validation studies, evaluated discriminatory power candidate proteins.Among 302 proteins analysed, 20 were identified as...
Recent advances in quantitative proteomic technology have enabled the large-scale validation of biomarkers. We here performed a analysis membrane fractions from colorectal cancer tissue to discover biomarker candidates, and then extensively validated candidate proteins identified. A total 5566 were identified six samples, each which was obtained polyps with without metastasis. GO cellular component predicted that 3087 these proteins, whereas TMHMM algorithm 1567 had transmembrane domain....
Abstract Oxidative stress contributes to the pathophysiology of a variety diseases, and circulating biomarkers its severity remains topic great interest for researchers. Our peptidomic strategy enables accurate reproducible analysis proteins/peptides with or without post-translational modifications. Conventional wisdom holds that hydrophobic methionines exposed an aqueous environment experimental handling procedures are vulnerable oxidation. However, we show mass spectra intensity ratio...
Since LC–MS-based quantitative proteomics has become increasingly applied to a wide range of biological applications over the past decade, numerous studies have performed relative and/or absolute abundance determinations across large sets proteins. In this study, we discovered prognostic biomarker candidates from limited breast cancer tissue samples using discovery-through-verification strategy combining iTRAQ method followed by selected reaction monitoring/multiple monitoring analysis...
The protein composition of gingival crevicular fluid (GCF) may reflect the pathophysiology periodontal diseases. A standard GCF proteomic pattern healthy individuals would serve as a reference to identify biomarkers diseases by proteome analyses. However, profiles obtained from apparently have not been well explored. As step toward detection for diseases, we applied both gel-based and gel-free methods analyze subjects compared with supragingival saliva. To ensure optimized extraction GCF,...
Numerous formaldehyde-fixed and paraffin-embedded clinical tissues have been created in the past decades stored pathological depositories at hospitals as well laboratories worldwide. In addition to archived tissues, formaldehyde-fixation is also mandatory for preparing proteomics samples from diseased patients or animal models order inactivate contagious agents. Protein extraction hampered by Schiff base formation between amino groups of proteins formaldehyde. Although achievement highest...
Periodontal disease is a bacterial infection that destroys the gingiva and surrounding tissues of oral cavity. Gingival crevicular fluid (GCF) extracted from gingival sulcus pocket. Analysis biochemical markers in GCF, which predict progression periodontal disease, may facilitate diagnosis. However, no useful GCF with high sensitivity for detecting have been identified. Thus, search continued interest experimental clinical research. Using tandem mass tag (TMT) labeling, we analyzed samples...
Abstract The epsin family of endocytic adapter proteins are widely expressed, and interact with both lipids to regulate a variety cell functions. However, the role epsins in atherosclerosis is poorly understood. Here, we show that deletion endothelial reduces inflammation attenuates using culture mouse models this disease. In atherogenic cholesterol-treated murine aortic cells, ubiquitinated endoplasmic reticulum protein inositol 1,4,5-trisphosphate receptor type 1 (IP3R1), which triggers...
Ezrin-radixin-moesin-binding phosphoprotein 50 (EBP50) is a scaffold protein that required for epithelial polarity. Knockout (KO) of membranous EBP50 (Me-EBP50) in ovarian clear cell carcinoma (OCCC) cells induced an epithelial-mesenchymal transition (EMT)-like phenotype, along with decreased proliferation, accelerated migration capability, and induction cancer stem (CSC)-like properties. Shotgun proteomics analysis proteins co-immunoprecipitated revealed Me-EBP50 strongly interacts myosin 9...
Objectives: To gain information about overexpressed antigens in renal cell carcinoma (RCC) by using a chemical proteomics approach. Methods: RCC line 769P was cultured and proteome analysis subsequently carried out the culture supernatants. By sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) tandem mass spectrometry (LC‐MS/MS), proteins supernatants were searched. A MEDLINE search to define functions of identified out. Results: Four differentially regulated (profilin 1,...
// Masashi Akiya 1 , Masaaki Yamazaki Toshihide Matsumoto Yusuke Kawashima 2 Yasuko Oguri Sabine Kajita Daiki Kijima Risako Chiba Ako Yokoi Hiroyuki Takahashi Yoshio Kodera and Makoto Saegusa Department of Pathology, Kitasato University School Medicine, Sagamihara, Kanagawa 252-0374, Japan Center for Disease Proteomics, Science, University, Correspondence to: Saegusa, email: msaegusa@med.kitasato-u.ac.jp Keywords: LEFTY, ovarian clear cell carcinoma, proliferation, apoptosis, TGF-beta...
Abstract Oxidative stress has been linked to a number of chronic diseases, and this aroused interest in the identification clinical biomarkers that can accurately assess its severity. We used liquid chromatography-high resolution mass spectrometry (LC-MS) show oxidised non-oxidised Met residues at position 147 human serum albumin (Met ) be reproducibly quantified with stable isotope-labelled peptides. oxidation was significantly higher patients diabetes than controls. Least square...
Recent studies utilizing single-cell analysis have unveiled the presence of various fibroblast (Fb) subsets within synovium under inflammatory conditions in osteoarthritis (OA), distinguishing them from those rheumatoid arthritis (RA). Moreover, it has been reported that pain knee OA patients is linked to specific subsets. Single-cell expression profiling methods offer an incredibly detailed view molecular states individual cells. However, one limitation these they require destruction cells...
FtsZ is highly conserved among bacteria and plays an essential role in bacterial cell division. The tense conformation of bound to GTP assembles into a straight filament via head-to-tail associations, then the upper subunit hydrolyzes lower subunit. with GDP disassembles accompanied by conformational change from relaxed conformation. Although crystal structures derived several species have been determined, has only observed Staphylococcus aureus (SaFtsZ). Recent cryo-electron microscopy...
We made a comprehensive study of protein expression androgen-independent (AI) prostate cancer by means agarose two-dimensional gel electrophoresis (agarose 2-DE) followed liquid chromatography-tandem mass spectrometric analysis. The 2-DE method being good at separating high molecular (HMM) proteins and alkaline ones, so we were able to successfully reveal differences between the proteomes androgen-dependent (AD) tumors those AI tumors. During creation maps, identified 295 (91.0%) out 324...
LC-ESI/MS/MS-based shotgun proteomics is currently the most commonly used approach for identification and quantification of proteins in large-scale studies biomarker discovery. In past several years, technologies have been refined toward further enhancement proteome coverage. complex series protocols involved proteomics, however, loss proteolytic peptides during lyophilization step prior to LC/MS/MS injection has relatively neglected despite fact that dissolution hydrophobic lyophilized...