A5068705608- Oral microbiology and periodontitis research
- Chemical synthesis and alkaloids
- Catalytic Cross-Coupling Reactions
- Axial and Atropisomeric Chirality Synthesis
- Synthesis and Biological Evaluation
- Cardiac Valve Diseases and Treatments
- Catalytic C–H Functionalization Methods
- Quinazolinone synthesis and applications
- Asymmetric Synthesis and Catalysis
- Streptococcal Infections and Treatments
- Respiratory and Cough-Related Research
- Asthma and respiratory diseases
- Advanced Synthetic Organic Chemistry
- Laser Applications in Dentistry and Medicine
- Synthetic Organic Chemistry Methods
- Cardiac and Coronary Surgery Techniques
- Cancer therapeutics and mechanisms
- Renal Transplantation Outcomes and Treatments
- Dialysis and Renal Disease Management
- Synthesis and biological activity
- Dental Implant Techniques and Outcomes
- Alkaloids: synthesis and pharmacology
- Synthesis and Reactions of Organic Compounds
- Renal Diseases and Glomerulopathies
- Oral Health Pathology and Treatment
Kitasato University
2016-2025
Tokyo Medical and Dental University
2015-2024
Institute of Science Tokyo
2024
Okayama University
2011-2023
The University of Tokyo
2017-2023
Komatsu (Japan)
2023
Nihon University
2023
JA Shizuoka Koseiren ENSHU hospital
2021-2022
Teikyo University
2012-2021
Kitasato University Hospital
2009-2020
The affinity of [(3)H]benzylpenicillin for penicillin-binding protein (PBP) 3A was reduced in 25 clinical isolates beta-lactamase-negative ampicillin (AMP)-resistant (BLNAR) Haemophilus influenzae which the AMP MIC > or =1.0 microg/ml. affinities PBP 3B and 4 were also some strains. sequences ftsI gene encoding transpeptidase domain and/or dacB determined these strains compared to those AMP-susceptible Rd BLNAR classified into three groups on basis deduced amino acid substitutions gene, is...
Several hydroxyproline (Hyp)-containing food-derived collagen peptides were identified in human blood after oral ingestion of gelatin hydrolysates. However, these types not quantified plasma. In this report, a sensitive LC-MS/MS method was introduced for simultaneous quantitative analysis Hyp-containing peptides. All peptide concentrations determined accurately, with all coefficients determination (r(2)) >0.999. The achieved detection and quantification limits 0.01 pmol/ml 12.5-1,000 plasma,...
The microbial differences between peri-implantitis and periodontitis in the same subjects were examined using 16S rRNA gene clone library analysis real-time polymerase chain reaction.Subgingival plaque samples taken from deepest pockets of sites six subjects. prevalence bacteria was analysed a reaction.A total 333 different taxa identified 799 sequenced clones; 231 (69%) uncultivated phylotypes, which 75 novel. numbers bacterial at 192 148 respectively. composition more diverse when compared...
Peri-implantitis (PI) is an inflammatory disease which leads to the destruction of soft and hard tissues around osseointegrated implants. The subgingival microbiota appears be responsible for peri-implant lesions although complexity has been reported in PI, PI not identified.The purpose this study was identify subjects who have clinically healthy implants, periodontitis-affected teeth using 16S rRNA gene clone library analysis clarify microbial differences.Three participated study....
Abstract Increasing evidence indicates that periodontitis affects non-alcoholic fatty liver disease (NAFLD). We examined the relationship between periodontal bacterial infection and clinical/biochemical parameters in 52 NAFLD patients. Anti- Aggregatibacter actinomycetemcomitans ( Aa ) antibody titers correlated positively with visceral fat, fasting plasma insulin, HOMA-IR; negatively liver/spleen ratio. C57BL/6J mice (8-weeks-old) were given or saline (control) for 6 weeks, fed either...
Abstract The quantity of periodontopathic bacteria in plaque samples is an important determinant for understanding the etiologic role bacteria. real‐time PCR method was used to detect and quantify bacteria, such as Actinobacillus actinomycetemcomitans, Bacteroides forsythus, Porphyromonas gingivalis, Treponema denticola , socranskii saliva subgingival samples. There good agreement between results conventional methods. Using LightCycler™ system we were able determine amount within hour....
Background: The aim of the present study was to identify Treponema socranskii in addition denticola and Porphyromonas gingivalis by polymerase chain reaction (PCR), clarify relationship between presence these microorganisms severity clinical periodontal parameters. Methods: Saliva subgingival plaque collected from 123 subjects (38 aggressive periodontitis patients, 65 chronic 20 healthy patients) were subjected PCR detect aforementioned 3 microorganisms. Results: Detection frequencies T....
To explore the structural requirements of (+)-cis-khellactone derivatives as novel anti-HIV agents, 24 monosubstituted 3',4'-di-O-(S)-camphanoyl-(+)-cis-khellactone (DCK) were synthesized asymmetrically. These compounds included 4 isomeric monomethoxy analogues (3−6), monomethyl (7−10), 4-alkyl/aryl-substituted (11−14), and 12 4-methyl-(+)-cis-khellactone (15−26) with varying 3',4'-substituents. screened against HIV-1 replication in acutely infected H9 lymphocytes. The results demonstrated...
A series of disubstituted 3',4'-di-O-(S)-camphanoyl-(+)-cis-khellactone (DCK) analogues (1−10) were synthesized and evaluated for inhibition HIV-1 replication in H9 lymphocytes. 5-Methoxy-4-methyl DCK (8) was the most promising compound with an EC50 value 7.21 × 10-6 μM a therapeutic index >2.08 10,7 which much better than those lead same assay. Another six (1−5 7) more potent AZT but less active DCK. Conformational analysis suggested that resonance coumarin system is essential structural...
Graphite oxide (GO) and its constituent layers (i.e., graphene oxide) display a broad range of functional groups and, as such, have attracted significant attention for use in numerous applications. GO is commonly prepared using the "Hummers method" or variant thereof which graphite treated with KMnO4 various additives H2SO4. Despite omnipresence, underlying chemistry such oxidation reactions not well understood typically affords results that are irreproducible some cases, unsafe. To overcome...
Abstract Polymicrobial diseases, which can be life threatening, are caused by the presence and interactions of multiple microbes. Peri-implantitis periodontitis representative polymicrobial diseases that show similar clinical symptoms. To establish a means differentiating between them, we compared microbial species functional genes in situ performing metatranscriptomic analyses peri-implantitis samples obtained from same subjects (n = 12 each). Although two differed terms 16S rRNA-based...
Many risk factors related to the development of non-alcoholic fatty liver disease (NAFLD) have been proposed, including most well-known diabetes and obesity as well periodontitis. As periodontal pathogenic bacteria produce endotoxins, treatment can result in endotoxemia. The aim this study was investigate effects intravenous, sonicated Porphyromonas gingivalis (Pg) injection on glucose/lipid metabolism, steatosis, gut microbiota mice. Endotoxemia induced C57BL/6J mice (8 weeks old) by...
Abstract DNA methyltransferases (DNMTs) catalyze methylation at the C5 position of cytosine with S -adenosyl- l -methionine. Methylation regulates gene expression, serving a variety physiological and pathophysiological roles. The chemical mechanisms regulating DNMT enzymatic activity, however, are not fully elucidated. Here, we show that protein S-nitrosylation cysteine residue in DNMT3B attenuates activity consequent aberrant upregulation expression. These genes include Cyclin D2 ( Ccnd2 ),...
Drug-induced tubulointerstitial injury is a common cause of renal impairment. Since the mechanisms drug-induced tubular are diverse, various treatment approaches needed according to pathogenesis. Renal biopsy indispensable determine not only pathological diagnosis, but also underlying mechanism, and guide appropriate treatment. Most recently, one red yeast supplements has been widely highlighted as novel damage, mainly in Japan Asia. However, neither detailed findings nor mechanism...