A5054752121- Wound Healing and Treatments
- Reconstructive Surgery and Microvascular Techniques
- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Periodontal Regeneration and Treatments
- Mesenchymal stem cell research
- Dermatologic Treatments and Research
- Reconstructive Facial Surgery Techniques
- Silk-based biomaterials and applications
- Surgical Sutures and Adhesives
- Streptococcal Infections and Treatments
- Cleft Lip and Palate Research
- Cardiac Arrest and Resuscitation
- Genetic and rare skin diseases.
- Diabetic Foot Ulcer Assessment and Management
- Breast Implant and Reconstruction
- Craniofacial Disorders and Treatments
- Cutaneous Melanoma Detection and Management
- 3D Printing in Biomedical Research
- Facial Nerve Paralysis Treatment and Research
- Photoacoustic and Ultrasonic Imaging
- Cellular Mechanics and Interactions
- Fibroblast Growth Factor Research
- Burn Injury Management and Outcomes
- dental development and anomalies
Kyoto University
2014-2025
Creative Commons
2018-2023
Kyoto Prefectural University of Medicine
2023
Committee on Publication Ethics
2023
Kyoto University of Education
2020-2023
Hospital de São João
2023
Jichi Medical University
2015-2022
Kansai Medical University
2013-2021
Kyoto Medical Center
2021
Hiroshima University Hospital
2021
Graphene oxide (GO) is widely recognized as a promising material in variety of fields, but its structure and composition has yet to be fully controlled. We have developed general strategies control the oxidation degree graphene-like materials via two methods: graphite by KMnO4 H2SO4 (oGO), reduction highly oxidized GO hydrazine (rGO). Even though oxygen content may same, oGO rGO different properties, for example adsorption ability, electron conductivity. These differences property arise from...
Platelet-rich plasma (PRP) is an autologous blood product that contains a high concentration of several growth factors. Platelet-derived factor (PDGF)-BB potential mitogen for human adipose-derived stem cells (hASCs). PRP stimulates proliferation hASCs; however, the signaling pathways activated by remain unclear. hASCs were cultured with or without PDGF-BB, and was assessed. also treated PDGF-BB imatinib, which PDGF receptor tyrosine kinase inhibitor, sorafenib, multikinase inhibitor....
The adipose tissue renin-angiotensin system (RAS) has been implicated in the pathophysiology of obesity and dysfunction tissue. However, neither regulation angiotensinogen (AGT) expression nor secretion tissue-derived AGT fully elucidated humans.Human subcutaneous abdominal (SAT) biopsies were performed for 46 subjects with a wide range body mass index (BMI). Considering mRNA level indices fat mass, amount (A-AGT-S) was estimated. Using mouse model weight reduction, plasma levels measured...
Chronic skin ulcers such as diabetic and venous leg are increasing a costly problem in healthcare. We have developed novel artificial dermis, collagen/gelatin sponge (CGS), which is capable of sustained release basic fibroblast growth factor (bFGF) for more than 10 days. The objective this study was to investigate the safety efficacy CGS impregnated with bFGF treatment chronic ulcers. Patients that had not healed at least 4 weeks were treated 7 or 14 μg/cm(2) after debridement, wound bed...
Graphite oxide (GO) and its constituent layers (i.e., graphene oxide) display a broad range of functional groups and, as such, have attracted significant attention for use in numerous applications. GO is commonly prepared using the "Hummers method" or variant thereof which graphite treated with KMnO4 various additives H2SO4. Despite omnipresence, underlying chemistry such oxidation reactions not well understood typically affords results that are irreproducible some cases, unsafe. To overcome...
Background Adipose tissue-derived stem cells (ASCs) have been recently isolated from human subcutaneous adipose tissue. ASCs may be useful in regenerative medicine as an alternative to bone marrow-derived cells. Changes the oxygen concentration influence physiological activities, such cell proliferation. However, effects of on remain unclear. In present study, hypoxia ASC proliferation were examined. Methods Normal tissue was collected lower abdomen, and prepared with collagenase treatment....
Tissue expanders represent one of the main surgical options for skin reconstruction in cases tumors, traumalike burn injury, scar contracture, and alopecia. However, tissue expander device is also associated with complications such as infection extrusion. The aim this study was to analyze risk factors major use pediatric patients using multivariate analysis.
PURPOSE: To investigate the relationship between metal content of hepatocellular carcinoma (HCC) and signal intensity pattern on magnetic resonance images. MATERIALS AND METHODS: The patterns 59 HCCs 3 cm in diameter or smaller were correlated with histologic findings content. RESULTS: high T1-weighted images demonstrated more steatosis (P = .035) higher copper .008) than did surrounding hepatic parenchyma. T2-weighted clear cells .001) intensities related to a lower degree differentiation,...
Artificial dermis (AD) has been used to regenerate dermis-like tissues in the treatment of full-thickness skin defects, but it takes 2 or 3 weeks complete dermal regeneration. Our previous study demonstrated that injection basic fibroblast growth factor (bFGF)–impregnated gelatin microspheres (MS) into AD accelerates regeneration tissue. However, MS before clinical use is complicated and time consuming. This investigated a new scaffold, which collagen are integrated, capable sustained bFGF...
The objective of this study was to evaluate the ability a scaffold, collagen–gelatin sponge (CGS), release basic fibroblast growth factor (bFGF) in sustained manner, using pressure-induced decubitus ulcer model involving genetically diabetic mice. We confirmed that CGSs impregnated with bFGF concentration up 50 µg/cm2 were able sustain throughout their biodegradation. prepared ulcers on After debriding ulcers, we implanted (diameter 8 mm) normal saline solution (NSS) or (7, 14, 28 µg/cm2)....
Introduction: A collagen/gelatin scaffold (CGS) can provide a sustained release of basic fibroblast growth factor (bFGF), which promotes wound healing. However, bFGF is approved for clinical use in Japan and China only. One potential alternative to platelet lysate (PL), safe easily attainable source wide range factors necessary tissue repair. In the present study, we investigated PL with CGS repair wounds identified optimal concentration Materials Methods: We generated from concentrated...
Indole readily undergoes nucleophilic substitution at the C3 site, and many indole derivatives have been functionalized using this property. also forms indolium, which allows electrophilic addition in acidic conditions, but current examples limited to intramolecular reactions. C2 site-selective fluoroalcohol a Lewis acid was developed.
Human platelet lysate (hPL) part of the growth factor cocktail derived from human platelets, which has been applied as a cell supplement. The production process is easier in comparison to platelet-rich plasma; thus, hPL now considered for use wound healing therapy. However, methods preserving more than several months that maintain its bioactivity must be considered, especially chronic treatment. present study compared effects preservation 9 using refrigerator or deep freezer. We investigated...
ABSTRACT Thinning of anterolateral thigh flap is challenging. Anatomical studies have shown variations in arterial branching patterns the subcutaneous layer, which were suspected to be reason for high frequency thinning failures. We attempted visualize courses preoperatively and perform perforator flaps using this information appropriately. accumulated evidence on accuracy noninvasive vascular visualization photoacoustic tomography (PAT). In present case, we applied a medical imaging...
Adipose tissue engineering requires effective strategies for regenerating adipose tissue, with adipose-derived stem cells (ASCs) being favored due to their robust self-renewal capacity and multipotent differentiation potential. In this study, the efficacy of poly-L-lactic acid (PLLA) mesh containing collagen sponge (CS), seeded ASCs promote formation, was investigated. PLLA-CS implants GFP-positive were inserted at high concentration (1 × 10
To overcome the absorption of traditional fat grafting, techniques for adipose-derived regenerative cell (ADRC)-enriched grafting are currently being adapted practical application. The Celution800/CRS (Cytori Therapeutics, San Diego, CA) has enabled rapid patient's own freshly harvested ADRCs without requiring a culturing step. However, optimal concentration and effects on characteristics grafted after free remain unclear.ADRCs were isolated purified from human tissue using Celution800/CRS....