A5105832217- Heart Failure Treatment and Management
- Regulation of Appetite and Obesity
- Adipose Tissue and Metabolism
- Hormonal Regulation and Hypertension
- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Nitric Oxide and Endothelin Effects
- Biochemical Analysis and Sensing Techniques
- Adipokines, Inflammation, and Metabolic Diseases
- Cardiac Fibrosis and Remodeling
- Pancreatic function and diabetes
- Renin-Angiotensin System Studies
- Peptidase Inhibition and Analysis
- Cardiovascular Function and Risk Factors
- Pituitary Gland Disorders and Treatments
- Blood Pressure and Hypertension Studies
- Metabolism, Diabetes, and Cancer
- Pulmonary Hypertension Research and Treatments
- Cardiac electrophysiology and arrhythmias
- Peroxisome Proliferator-Activated Receptors
- Ion channel regulation and function
- Cardiomyopathy and Myosin Studies
- Signaling Pathways in Disease
- Diet and metabolism studies
- Thyroid Disorders and Treatments
Kyoto University
2015-2025
Kyoto Medical Center
2014-2021
Kyoto University Hospital
1994-2017
National Cerebral and Cardiovascular Center
2000-2017
Shinshu University
2017
Techniker Krankenkasse (Germany)
2016
Matsumoto University
2014-2015
Shiga University of Medical Science
2012-2015
Kyoto College of Medical Science
1995-2013
Nagasaki University Hospital
2008-2013
Using a specific radioimmunoassay for human brain natriuretic peptide (hBNP) with monoclonal antibody, we have investigated its synthesis, secretion, and clearance in comparison those of atrial (ANP) normal subjects patients congestive heart failure (CHF). Mean BNP-like immunoreactivity (-LI) levels atrium ventricle were 250 18 pmol/g, respectively. The plasma BNP-LI level was 0.90 +/- 0.07 fmol/ml, which 16% the ANP-LI level. In contrast, markedly increased CHF proportion to severity,...
Ghrelin, an endogenous ligand for the GH secretagogue receptor, was isolated from rat stomach and is involved in a novel system regulating release. Although previous studies rodents suggest that ghrelin also energy homeostasis secretion influenced by feeding, little known about plasma humans. To address this issue, we studied ghrelin-like immunoreactivity levels elucidated source of circulating effects feeding state on The concentration normal humans measured specific RIA 166.0 +/- 10.1...
Ghrelin, an endogenous ligand for growth hormone secretagogue (GHS)receptor originally isolated from the stomach, occurs in hypothalamic arcuate nucleus and may play a role energy homeostasis. Synthetic GHSs have activated neurons containing neuropeptide Y (NPY),suggesting involvement of NPY some ghrelin actions. This study was designed to elucidate regulation food intake. A single intracerebroventricular (ICV) injection (5-5,000 ng/rat)caused significant dose-related increase cumulative...
Coronary spasm plays an important role in the pathogenesis of ischemic heart diseases general. However, precise mechanism(s) responsible for coronary remains to be elucidated, and we examined molecular genetics spasm.We searched possible mutations endothelial nitric oxide synthase (eNOS) gene patients with spasm. In this study, demonstrate existence 3 linked 5'-flanking region eNOS (T-786-->C, A-922-->G, T-1468-->A). The incidence was significantly greater than control group (P<0.0001)....
Abstract Bisphenol A (BPA), a monomer of polycarbonate plastics, has been shown to possess estrogenic properties and act as an agonist for the estrogen receptors. Although epidemiologically based investigation suggested that some chemicals could disrupt thyroid function in animals, effects on hormone receptors (TRs) are unknown. We show here BPA inhibits TR-mediated transcription by acting antagonist. In transient gene expression experiments, suppressed transcriptional activity is stimulated...
We recently identified a novel human protein kinase, p160 ROCK, as putative downstream target of the small GTPase Rho. Using ROCK cDNA probe, we isolated two distinct, highly related sequences from mouse libraries. One encoded counterpart (ROCK‐I), and other ROCK‐related kinase (ROCK‐II). Like ROCK/ROCK‐I, ROCK‐II also bound to GTP‐Rho selectively. ROCK‐I mRNA was ubiquitously expressed except in brain muscle, whereas abundantly brain, heart, lung placenta. These results suggest that at...
To elucidate the ligand-receptor relationship of natriuretic peptide system, which comprises at least three endogenous ligands, atrial (ANP), brain (BNP), and C-type (CNP), receptors, ANP-A receptor or guanylate cyclase-A (GC-A), ANP-B cyclase-B (GC-B), clearance (C-receptor), we characterized preparations from human, bovine, rat tissues cultured cells with aid binding assay, Northern blot technique, cGMP production method. Using these preparations, examined affinities ANP, BNP, CNP for...
We previously demonstrated that brain natriuretic peptide (BNP) is a cardiac hormone mainly produced in the ventricle, while major production site of atrial (ANP) atrium. To assess pathophysiological role BNP ventricular overload, we have examined gene expression BNP, In comparison with ANP, model hypertrophy using cultured neonatal rat cardiocytes. During cardiocyte evoked by endothelin-1, Phenylephrine, or PMA, steady state level mRNA increased as rapidly "immediate-early" induction c-fos...
BACKGROUND The plasma levels of atrial natriuretic peptide (ANP) and brain (BNP) are increased in relation to the severity congestive heart failure (CHF). This study was designed examine whether secretion patterns ANP BNP vary with underlying cardiac disorders CHF different degrees overload atria ventricles. METHODS AND RESULTS We measured aorta 20 patients mitral stenosis (MS) whom mainly overloaded, 30 dilated cardiomyopathy (DCM) both ventricles control subjects during catheterization....
Cardiac fibrosis, defined as a proliferation of interstitial fibroblasts and biosynthesis extracellular matrix components in the ventricles heart, is consequence remodeling processes initiated by pathologic events associated with variety cardiovascular disorders, which leads to abnormal myocardial stiffness and, ultimately, ventricular dysfunction. Brain natriuretic peptide (BNP) cardiac hormone produced primarily myocytes, its plasma concentrations are markedly elevated patients congestive...
BACKGROUND Brain natriuretic peptide is a novel that secreted predominantly from the ventricles, and its plasma levels have been shown to be markedly increased in patients with chronic congestive heart failure. This study was designed examine of brain as well atrial acute myocardial infarction. METHODS AND RESULTS We examined 50 consecutive (36 men 14 women; mean age, 66 years) infarction over time course 4 weeks. The level significantly on admission compared controls (92 +/- 28 versus 5.2...
Atrial and brain natriuretic peptides (ANP BNP) are produced by the heart, their plasma concentrations increased in human chronic congestive heart failure. Although separate studies have suggested that circulating levels of biologically active C-terminal ANP, inactive N-terminal BNP may diagnostic utility detection left ventricular systolic dysfunction or hypertrophy, no directly assessed relative value these prospectively. We therefore designed this study to compare ability different detect...
C-type natriuretic peptide (CNP), the third member of family, is thus far known to be distributed mainly in central nervous system and considered act as a neuropeptide, contrast atrial (ANP) brain (BNP), which cardiac hormones. Recently, we others have demonstrated that ANP-B receptor, selectively activated by CNP, localized not only but peripheral tissues, including blood vessels. This finding has made us speculate regarding production CNP. In present study, cultured endothelial cells were...
Abstract —Essential hypertension has a genetic basis. Accumulating evidence, including findings of elevation arterial blood pressure in mice lacking the endothelial nitric oxide synthase (eNOS) gene, strongly suggests that alteration NO metabolism is implicated hypertension. There are, however, no reports indicating polymorphism eNOS gene associated with essential We have identified missense variant, Glu298Asp, exon 7 and demonstrated it both coronary spastic angina myocardial infarction. To...
To explore the pathophysiological role of leptin in obesity-related hypertension, we examined cardiovascular phenotypes transgenic skinny mice whose elevated plasma concentrations are comparable to those seen obese subjects. We also studied genetically KKAy with hyperleptinemia, which hypothalamic melanocortin system is antagonized by ectopic expression agouti protein. Systolic blood pressure (BP) and urinary catecholamine excretion relative nontransgenic littermates. The BP elevation...
Longitudinal bone growth is determined by endochondral ossification that occurs as chondrocytes in the cartilaginous plate undergo proliferation, hypertrophy, cell death, and osteoblastic replacement. The natriuretic peptide family consists of three structurally related endogenous ligands, atrial, brain, C-type peptides (ANP, BNP, CNP), thought to be involved a variety homeostatic processes. To investigate physiological significance CNP vivo , we generated mice with targeted disruption (...
Background —Obstructive sleep apnea syndrome (OSAS) is a common disorder in obese subjects. Visceral fat accumulation (VFA) better predictor of coronary heart disease than body mass index. Leptin hormone involved the control weight and distribution. The effect nasal continuous positive airway pressure (NCPAP) treatment on VFA serum leptin levels OSAS patients has not been known. Methods Results —VFA subcutaneous (SFA) were assessed by CT before after NCPAP 22 (mean hypopnea index >50...
Background —Vascular endothelial growth factor (VEGF) is an important angiogenic reported to induce migration and proliferation of cells, enhance vascular permeability, modulate thrombogenicity. VEGF expression in cultured cells (smooth muscle macrophages, cells) controlled by factors cytokines. Hence, the question arises whether could play a role atherogenesis. Methods Results —Frozen sections from 38 coronary artery segments were studied. The specimens characterized as normal with diffuse...
Background We have demonstrated that brain natriuretic peptide (BNP) is a cardiac hormone predominantly synthesized in and secreted from the ventricle. also reported that, compared with atrial (ANP), plasma concentration of BNP increased to greater degree patients congestive heart failure more rapidly acute myocardial infarction (AMI). Methods Results To investigate ventricular gene expression AMI, we analyzed concentrations along mRNA rats AMI produced by coronary artery ligation. The left...
The obese (ob) gene, the mutation of which results in severe hereditary obesity and diabetes mice, has recently been isolated through positional cloning. In this study, we a full-length human ob complementary DNA (cDNA) clone examined tissue distribution gene expression humans. nucleotide sequences cDNA coding region were 83% identical to those mouse rat regions. Analysis deduced amino acid revealed that protein is 166–amino polypeptide with putative signal sequence 84 homologous proteins,...