The characteristics of the fusiform species Bacteroides forsythus, isolated from human periodontal pockets, were examined. 165 rDNA sequence analysis confirmed that B. forsythus was not a within genus sensu stricto. Although phylogenetically related to distasonis and merdae in phylogenetic tree, ratio anteiso-15:0 iso-15:0 whole-cell methanolysates different those distasonis, other species. did grow on medium containing 20% bile, but members fragilis group did. only tested trypsin-positive...

T he risk for harboring 6 putative periodontal pathogens in 4 selected pockets, whole saliva, or either site (i.e., orally) was determined 52 Caucasians, 49 African‐Americans, 48 Asian‐Americans, and 50 Hispanics living Los Angeles. 16S rRNA PCR analysis assessed the presence of Actinobacillus actinomycetemcomitans, Bacteroides forsythus, Porphyromonas gingivalis, Prevotella intermedia, nigrescens , Treponema denticola . Step‐wise regression relationship between occurrence each organism...

The 2011 Great East Japan Earthquake that occurred on March 11, 2011, had magnitude of 9.0 the Richter Scale with epicenter approximately 70 km east Oshika Peninsula in Miyagi Prefecture. This earthquake triggered terrible tsunami waves which hit coast and propagated around Pacific Ocean. caused extensive severe infrastructural damage, such as damages coastal protection structures buildings, significantly changed river morphology. paper presents tsunami-induced estuarine morphology changes...

Background: Various mammalian viruses and specific bacteria seem to play important roles in the pathogenesis of human periodontitis. This study examined relationship between subgingival herpesviruses periodontal disease potential periodontopathic 140 adults exhibiting either periodontitis or gingivitis. Methods: A nested‐polymerase chain reaction (PCR) method determined presence Epstein‐Barr virus type 1 2 (EBV‐1, EBV‐2), cytomegalovirus (HCMV), herpes simplex (HSV) a 16S rRNA PCR detection...

Abstract The quantity of periodontopathic bacteria in plaque samples is an important determinant for understanding the etiologic role bacteria. real‐time PCR method was used to detect and quantify bacteria, such as Actinobacillus actinomycetemcomitans, Bacteroides forsythus, Porphyromonas gingivalis, Treponema denticola , socranskii saliva subgingival samples. There good agreement between results conventional methods. Using LightCycler™ system we were able determine amount within hour....

Background: The aim of the present study was to identify Treponema socranskii in addition denticola and Porphyromonas gingivalis by polymerase chain reaction (PCR), clarify relationship between presence these microorganisms severity clinical periodontal parameters. Methods: Saliva subgingival plaque collected from 123 subjects (38 aggressive periodontitis patients, 65 chronic 20 healthy patients) were subjected PCR detect aforementioned 3 microorganisms. Results: Detection frequencies T....

T his study compared the presence of 6 periodontopathic bacteria in whole saliva and subgingival plaque 202 subjects. The test were identified using a 16S rRNA‐based PCR detection method. Each subject contributed sample paper point pooled from deepest periodontal pocket each quadrant dentition. kappa revealed fair agreement between Porphyromonas gingivalis, Prevotella intermedia , Treponema denticola samples (kappa > 0.4). McNemar showed that differences types due to more frequent 3...

Background: Diabetes mellitus (DM) is a major risk factor for periodontal disease and affects various cellular functions. Periodontal ligament stem cells (PDLSCs) play an important role in tissue regeneration; however, the effect of hyperglycemia on PDLSCs unclear. The aim this study to investigate whether regeneration, using human high‐glucose medium as model DM. Methods: were obtained from healthy adult mandibular third molars. Cell proliferation, osteoblastic differentiation,...

Terminal restriction fragment-length polymorphism (T-RFLP) analysis was applied to characterize oral bacterial flora in saliva from 18 healthy subjects and patients with periodontitis. The 16S rRNA genes (rDNAs) of bacteria spirochaetes were amplified by PCR a 6′carboxy-fluorescein (6-FAM)-labelled universal forward primer (27F) reverse (1492R) or the Spirochaeta-selective primer. rDNAs digested enzymes 4 bp recognition sites (HhaI MspI) analysed using an automated DNA sequencer. T-RFLP...

Background: Porphyromonas gingivalis, Bacteroides forsythus , and Actinobacillus actinomycetemcomitans have been described as periodontopathic bacteria, their presence in subgingival pockets can lead to development of periodontal disease. Until now, clinical parameters used evaluate the effect conventional treatment without microbiological parameters. The present study examined effects initial therapy using DNA probes polymerase chain reaction (PCR). Methods: Twenty‐six patients with...

Abstract The oral bacterial flora in the saliva from two patients with periodontitis and a periodontally healthy subject were compared using sequence analysis of 16S rDNA libraries without cultivation. rDNAs amplified salivary DNA by PCR cloned. Randomly selected clones partially sequenced. On basis similarities, classified into several clusters corresponding to major phylum domain Bacteria . was low G+C Gram‐positive bacteria. There no clonal affiliated periodontopathic bacteria sample...

Helicobacter pylori has been associated with the development of peptic ulcers and gastric cancer. Although it may be transmitted through oral cavity, is unknown whether cavity acts as a permanent reservoir for this bacterium. The purpose study was to use nested polymerase chain reaction (PCR) clarify H. pylori.The existence in determined by PCR 57 subjects culture method 18 subjects. presence periodontopathic bacteria also 16S rRNA-based method.Although rarely detected technique, frequently...

Actinobacillus actinomycetemcomitans is considered a major etiologic agent of aggressive periodontitis. Other periodontopathic bacteria such as Porphyromonas gingivalis are also suspected participating in periodontitis, although the evidence controversial. The aim present study was to investigate prevalence and clarify microbiological features periodontitis Japanese patients.Subgingival plaque collected from 50 (AgP) patients (localized 10, generalized 40). Samples 35 chronic (CP) 18 healthy...

Abstract Background: The aim of this randomized controlled clinical trial was to determine the effects single‐visit full‐mouth ultrasonic debridement versus quadrant‐wise therapy. Material and Methods: Thirty‐six subjects with chronic periodontitis, were randomly allocated three groups – debridement, povidone iodine water. Whole‐mouth plaque, bleeding on probing (BOP), pocket depth attachment level recorded before treatment 1, 3 6 months post‐treatment. Plaque saliva samples collected for...

Terminal RFLP (T-RFLP) analysis was used to investigate changes in the oral microbiota saliva and subgingival plaque samples from one patient with aggressive periodontitis (subject A) two patients chronic (subjects B C) before 3 months after periodontal treatment. Substantial T-RFLP patterns of subjects C were noted improved hygiene full-mouth supra- scaling root planing. However, there little change subject A. Although proportions terminal restriction fragments (T-RFs) larger than 1000 bp...

Regenerative medicine is emerging as a promising option, but the potential of autologous stem cells has not been investigated well in clinical settings periodontal treatment. In this study, we evaluated safety and efficacy new regenerative therapy based on surgical implantation mesenchymal (MSCs) with biodegradable three‐dimensional (3D) woven‐fabric composite scaffold platelet‐rich plasma (PRP). Ten patients periodontitis, who required procedure for intrabony defects, were enrolled phase...

papillon‐lefèvre syndrome (pls) is a rare disease associated with the early onset of periodontal breakdown in deciduous and permanent dentition. The etiology destruction has not been completely clarified. Two female patients (ages 4 7 years) severe structures were examined. Except for palmar plantar hyperkeratosis, dermatologie examination revealed no other medical disorders. On immunological analysis, measurement serum antibody titers to periodontopathic bacteria‐ including Porphyromonas...