A5064494933- Streptococcal Infections and Treatments
- Escherichia coli research studies
- Antimicrobial Resistance in Staphylococcus
- Viral gastroenteritis research and epidemiology
- Infective Endocarditis Diagnosis and Management
- Bacteriophages and microbial interactions
- Neonatal and Maternal Infections
- Bacterial Infections and Vaccines
- Vibrio bacteria research studies
- Genomics and Phylogenetic Studies
- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Microbial Community Ecology and Physiology
- Aquaculture disease management and microbiota
- Salmonella and Campylobacter epidemiology
- Mycobacterium research and diagnosis
- Cellular transport and secretion
- Microbial infections and disease research
- Oral microbiology and periodontitis research
- Gut microbiota and health
- Autophagy in Disease and Therapy
- Polar Research and Ecology
- Legionella and Acanthamoeba research
- Nanopore and Nanochannel Transport Studies
- Vector-borne infectious diseases
Kyoto University
2016-2025
University of Miyazaki
2009-2022
Frontier Science Foundation
2015
Abstract A ‘sibling’ species of the model organism Caenorhabditis elegans has long been sought for use in comparative analyses that would enable deep evolutionary interpretations biological phenomena. Here, we describe first sibling C . , C. inopinata n. sp., isolated from fig syconia Okinawa, Japan. We investigate morphology, developmental processes and behaviour which differ significantly those The 123-Mb genome was sequenced assembled into six nuclear chromosomes, allowing delineation...
Abstract Enterohemorrhagic E. coli (EHEC) causes diarrhea and hemorrhagic colitis with life-threatening complications, such as hemolytic uremic syndrome. Their major virulence factor is Shiga toxin (Stx), which encoded by bacteriophages. Of the two types of Stx, production Stx2, particularly that Stx2a (a subtype Stx2), a risk for severe EHEC infections, but Stx2 level highly variable between strains. Here, we define four minor subtypes Stx2a-encoding phages according to their replication...
Escherichia albertii is a recently recognized close relative of coli . This emerging enteropathogen possesses type III secretion system (T3SS) encoded by the locus enterocyte effacement, similar to enteropathogenic and enterohemorrhagic E. (EPEC EHEC). Shiga toxin-producing strains have also been identified. The genomic features , particularly differences from other species, not yet well clarified. Here, we sequenced genome 29 (3 complete 26 draft sequences) isolated multiple sources...
Abstract Polymicrobial diseases, which can be life threatening, are caused by the presence and interactions of multiple microbes. Peri-implantitis periodontitis representative polymicrobial diseases that show similar clinical symptoms. To establish a means differentiating between them, we compared microbial species functional genes in situ performing metatranscriptomic analyses peri-implantitis samples obtained from same subjects (n = 12 each). Although two differed terms 16S rRNA-based...
Abstract The development and application of next-generation sequencing (NGS) have enabled comprehensive analyses the microbial community through extensive parallel sequencing. Current eukaryotic are primarily based on polymerase chain reaction amplification 18S rRNA gene (rDNA) fragments. We found that widely-used rDNA primers can amplify numerous stretches bacterial 16S gene, preventing high-throughput detection rare species, particularly in bacteria-rich samples such as faecal material. In...
Escherichia coli can cause extraintestinal infections in humans and animals. The hlyF gene is epidemiologically associated with virulent strains of avian pathogenic E. human neonatal meningitis–associated coli. We demonstrated that culture supernatants expressing HlyF induced autophagy eukaryotic cells. This phenotype coincided an enhanced production outer membrane vesicles (OMVs) by bacteria HlyF. protein displays a predicted catalytic domain the short-chain dehydrogenase/reductase...
It is generally difficult to specify the sources of infection by which domestic animals may acquire pathogens. Through 16S rRNA gene amplicon sequencing, we compared composition microbiota in saliva, vaginal mucus, and feces pigs, swabs feeder troughs water dispensers collected from pig farms Vietnam. The differed between samples each sample group. Streptococcus, Actinobacillus, Moraxella, Rothia were most abundant genera significantly discriminative saliva samples, regardless plasticity...
Abstract Invading microbial pathogens can be eliminated selectively by xenophagy. Ubiquitin-mediated autophagy receptors are phosphorylated TANK-binding kinase 1 (TBK1) and recruited to ubiquitinated bacteria facilitate autophagosome formation during xenophagy, but the molecular mechanism underlying TBK1 activation in response infection is not clear. Here, we show that bacterial increases Ca 2+ levels activate for xenophagy via -binding protein TBC1 domain family member 9 (TBC1D9)....
Incidence of Streptococcus dysgalactiae subspecies equisimilis (SDSE) bacteremia is increasing in the Kyoto-Shiga region Japan. We retrospectively analyzed clinical features SDSE and conducted comparative genomic analyses isolates collected from 146 episodes among 133 patients during 2005-2021. Of those patients, 7.7% required vasopressor support, 7.0% died while hospital. The prevalence resistant to erythromycin, minocycline, clindamycin increased 8.6% 2005-2017 21.6% 2018-2021. Our...
Abstract CprA is a short‐chain dehydrogenase/reductase (SDR) that contributes to resistance against colistin and antimicrobial peptides. The cprA gene conserved across Pseudomonas aeruginosa clades its expression directly regulated by the two‐component system PmrAB. We have shown leads production of outer membrane vesicles (OMVs) block autophagic flux greater capacity activate non‐canonical inflammasome pathway. In murine model sepsis, P. strain deleted for was less virulent than wild‐type...
ABSTRACT Enterohemorrhagic Escherichia coli O157 (EHEC O157) is a food-borne pathogen that has raised worldwide public health concern. The development of simple and rapid strain-typing methods crucial for the detection surveillance EHEC outbreaks. In present study, we developed multiplex PCR-based method O157, which based on variability in genomic location IS 629 among strains. This very simple, procedures are completed within 2 h, analysis can be performed without need special equipment or...
Phages and plasmids play important roles in bacterial evolution diversification. Although many draft genomes have been generated, phage plasmid are usually fragmented, limiting our understanding of their dynamics. Here, we performed a systematic analysis 239 7 complete Shiga toxin (Stx)-producing Escherichia coli O145:H28, the major virulence factors which encoded by prophages (PPs) or plasmids. The results indicated that PPs more stably maintained than A set ancestrally acquired was well...
Soil-transmitted helminths (STHs) are medically important parasites that infect 1.5 billion humans globally, causing a substantial disease burden. These the gastrointestinal tract of their host where they co-exist and interact with gut bacterial flora, leading to coevolution parasites, microbiota organisms. However, little is known about how these interactions change through time progression infection. Strongyloidiasis human parasitic caused by nematode Strongyloides stercoralis infecting...
Serratia marcescens is an important nosocomial pathogen causing various opportunistic infections, such as urinary tract bacteremia and sometimes even hospital outbreaks. The recent emergence spread of multidrug-resistant (MDR) strains further pose serious threats to global public health. This bacterium also ubiquitously found in natural environments, but the genomic differences between clinical environmental isolates are not clear, including those S. its close relatives. In this study, we...
Two prominent virulence factors of group A Streptococcus (GAS), streptolysin O (SLO) and NAD-glycohydrolase (Nga), are linked to enhanced pathogenicity the prevalent GAS strains. Recent advances show that SLO Nga important for intracellular survival in epithelial cells macrophages.
Streptococcus suis, an important zoonotic pathogen, is known to have open pan-genome and develop a competent state. In S. limited genetic lineages are suggested be associated with zoonosis. However, little about the evolution of diversified their respective phenotypic or ecological characteristics. this study, we performed comparative genome analyses focus on competence genes, mobile elements, elements related various defense systems against exogenous DNAs (defense elements) that gene...
Among three haemolysins identified thus far in Escherichia coli, alpha-haemolysin (HlyA) is encoded on the pathogenicity islands of extraintestinal pathogenic strains, while enterohaemolysin (EhxA) virulence plasmids enterohaemorrhagic E. coli (EHEC) strains. In contrast, gene for haemolysin E (HlyE) located chromosome backbone and therefore widely distributed among However, because hlyE expression repressed by H-NS protein has been disrupted many its haemolytic activity cannot be detected...
OBJECTIVE To evaluate colonization of Streptococcus suis and parasuis on pig farms in Japan to identify sources infections. SAMPLE Saliva, feces, vaginal swab samples from 84 healthy pigs several growth stages 4 feed troughs water dispensers at those farms. PROCEDURES Samples were collected August 2015 June 2016. Two quantitative PCR (qPCR) assays (one for S the other parasuis) designed use study. The novel qPCR used combination with previously described serotype 2 or 1/2 total bacteria....
Most bacteria naturally release spherical lipid-bilayered extracellular vesicles (EVs) containing proteins, nucleic acids, and virulence-related molecules, thus contributing to diverse biological functions including transport of virulence factors. The group A streptococcus, Streptococcus pyogenes (GAS), a major human pathogen, also releases EVs; however, it remains unclear how GAS EVs interact physiologically pathologically with host cells, what the differences are between invasive...
Group A Streptococcus (GAS) is a major human pathogen, which associated with wide spectrum of invasive diseases, such as pharyngitis, scarlet fever, rheumatic and streptococcal toxic shock syndrome (STSS). It hypothesized that differences in GAS pathogenicity are related to the acquisition diverse bacteriophages (phages). Nevertheless, genome also harbors clustered regularly interspaced short palindromic repeats (CRISPR) CRISPR-associated (cas) genes, play an important role eliminating...
Shiga toxin (Stx)-producing Escherichia coli (STEC) are foodborne pathogens causing serious diseases, such as haemorrhagic colitis and haemolytic uraemic syndrome. Although O157:H7 STEC strains have been the most prevalent, incidences of infections by several other serotypes recently increased. O121:H19 is one these major non-O157 STECs, but systematic whole genome sequence (WGS) analyses not yet conducted on this STEC. Here, we performed a global WGS analysis 638 strains, including 143...
Approximately 200 O-serogroups of Vibrio cholerae have already been identified; however, only 2 serogroups, O1 and O139, are strongly related to pandemic cholera. The study non-O1 non-O139 strains has hitherto limited. Nevertheless, there other clinically epidemiologically important serogroups causing outbreaks with cholera-like disease. Here, we report a comprehensive genome analysis the whole set V. O-serogroup reference provide an overview this bacterial pathogen. It revealed structural...