A5042924461- Nematode management and characterization studies
- Cancer, Hypoxia, and Metabolism
- Immune cells in cancer
- Entomopathogenic Microorganisms in Pest Control
- Parasite Biology and Host Interactions
- Cancer Research and Treatments
- Genetics, Aging, and Longevity in Model Organisms
- Insect and Arachnid Ecology and Behavior
- Insect symbiosis and bacterial influences
- Insect Resistance and Genetics
- Cancer, Lipids, and Metabolism
- Helminth infection and control
- Insect-Plant Interactions and Control
- Legume Nitrogen Fixing Symbiosis
- Evolution and Genetic Dynamics
- Genomics and Phylogenetic Studies
- Insect and Pesticide Research
- Parasites and Host Interactions
- Forest Insect Ecology and Management
- Cytokine Signaling Pathways and Interactions
- S100 Proteins and Annexins
- Spaceflight effects on biology
- Genetic and Environmental Crop Studies
- Algorithms and Data Compression
- Primate Behavior and Ecology
University of Miyazaki
2014-2024
Kumamoto University
2023
The University of Tokyo
2022
Forestry and Forest Products Research Institute
2012-2022
Düzce Üniversitesi
2022
Taisei (Japan)
2022
Ryukoku University
2021
Saga University
2011-2020
Max Planck Institute for Developmental Biology
2014
Tsurumi University
2013
Abstract A ‘sibling’ species of the model organism Caenorhabditis elegans has long been sought for use in comparative analyses that would enable deep evolutionary interpretations biological phenomena. Here, we describe first sibling C . , C. inopinata n. sp., isolated from fig syconia Okinawa, Japan. We investigate morphology, developmental processes and behaviour which differ significantly those The 123-Mb genome was sequenced assembled into six nuclear chromosomes, allowing delineation...
Species with broad ecological amplitudes respect to a key focal resource, niche generalists, should maintain larger and more connected populations than specialists, leading the prediction that nucleotide diversity will be lower subdivided in specialists relative their generalist relatives. This logic describes specialist-generalist variation hypothesis (SGVH). Some outbreeding species of Caenorhabditis nematodes use variety invertebrate dispersal vectors have high molecular diversity. By...
Parasite diversity has important implications in several research fields including ecology, evolutionary biology and epidemiology. Wide-ranging analysis been restricted because of the difficult, highly specialised time-consuming processes involved parasite identification. In this study, we assessed wild rats using 18S rDNA-based metagenomics. rDNA PCR products were sequenced an Illumina MiSeq sequencer sequences QIIME software successfully classified them into groups. The comparison results...
The co-existence of males, females and hermaphrodites, a rare mating system known as trioecy, has been considered an evolutionarily transient state. In nematodes, androdioecy (males/hermaphrodites) found in Caenorhabditis elegans, is thought to have evolved from dioecy (males/females) through trioecious intermediate. Thus, species are good models understand the steps requirements for evolution new systems. Here we describe two nematodes with Auanema rhodensis A. freiburgensis. Along...
Canthin-6-one (I), canthin-6-one 3-oxide (III) and new alkaloid, 1-methoxycanthin-6-one (II) were isolated from the wood of Ailanthus altissima SWINGLE (Simaroubaceae). The structure II was elucidated by spectroscopic studies chemical evidences.
A new nematode species, Bursaphelenchus sycophilus n. sp. is described. The species was found in syconia of a fig Ficus variegata during field survey fig-associated nematodes Japan. Because it has well-developed stylet and pharyngeal glands, the considered an obligate plant parasite, easily distinguished from all other fungal-feeding genus based upon these characters. Although B. shares important typological character, male spicule possessing strongly recurved condylus, with "B. eremus...
A survey of stag beetle (Lucanidae)-associated nematodes was conducted in Japan to construct a preliminary species inventory and obtain their molecular profiles as first step for elucidating host fidelity ecological roles. Eight beetles (94 individuals total), Dorcus rubrofemoratus, D. montivagus, striatipennis, rectus, titanus, Prismognathus angularis, Prosopocoilus inclinatus,and Lucanus maculifemoratus were collected from 12 localities Japan, dissected examined nematode associates....
Summary Drastic physiological and morphological changes in parasites are crucial for the establishment of a successful infection. The nematode B ursaphelenchus xylophilus is pathogenic agent pine wilt disease, little known about physiology morphology this at initial stage In study, we devised an infection system using stem cuttings that allowed us to observe transcriptional host‐infecting phytophagous phase. We found 60 genes enriched xenobiotic detoxification were up‐regulated two...
Taphrina fungi are biotrophic plant pathogens that cause deformity diseases. We sequenced the genomes of four species—Taphrina wiesneri, T. deformans, flavorubra, and populina—which parasitize Prunus, Cerasus, Populus hosts with varying severity disease symptoms. High levels gene synteny within species were observed, our comparative analysis further revealed these may utilize multiple strategies in coping host environment also found some specialized dimorphic species. These include...
The helminth Strongyloides stercoralis, which is transmitted through soil, infects 30–100 million people worldwide. S. stercoralis reproduces sexually outside the host as well asexually within host, causes a life-long infection. To understand population structure and transmission patterns of this parasite, we re-sequenced genomes 33 individual nematodes collected in Myanmar (prevalent region) Japan (non-prevalent region). We utilised method combining whole genome amplification...
The pine wood nematode Bursaphelenchus xylophilus is the causal agent of wilt disease, one most devastating forest diseases in East Asian and West European countries. lifecycle B. includes four propagative larval stages gonochoristic adults which are involved pathogenicity, two dispersal larvae spread disease. To elucidate ecological roles each developmental stage pathogenic life cycle, we performed a comprehensive transcriptome analysis using RNA-seq generated from all compared...
A new species of diplogastrid nematode, isolated in a previous survey nematodes associated with stag beetles Japan, is described as Parapristionchus giblindavisi n. gen., sp. gen. differs from other genera chiefly by its stomatal morphology. Distinguishing the genus are presence claw-like dorsal tooth both eurystomatous and stenostomatous forms division cheilostom into 12 plates lacking apical flaps. According to phylogenetic analysis nine ribosomal protein gene sequences, shows deep...
Summary Host recognition is critical during the phoretic stage of nematodes because it facilitates their association with hosts. However, limited information available on direct cues used for host and specificity in nematodes. Caenorhabditis japonica forms an intimate burrower bug Parastrachia japonensis. C. dauer larvae (DL), nematode, are mainly found adult P. japonensis female but no other species. To understand mechanisms species-specific carrier-biased ectophoresy japonica, we...
A Bursaphelenchus species was isolated from a Japanese native wood-boring weevil, Niphades variegatus , and dead Pinaceae trees. The nematode is associated with the weevils as dauer (dispersal third stage) juveniles dauers enter weevil tracheal system forming an abnormal expansion on trachea (atrium). Thus, hypothesised to be amensal/phoretic associate of because appeared inhibit respiration. propagative stages are trees (wood bark materials) thought feed there naturally propagated fungi....
The nematode family Aphelenchoididae is a highly divergent group that contains plant parasites, predators, fungal feeders, and insect parasites. It taxonomically separated into 7 subfamilies. Although molecular phylogenetic relationships among 6 of the subfamilies have been clarified, position subfamily Entaphelenchinae, which composed endoparasites insects, remains unclear. Here, new entaphelenchid species, Peraphelenchus orientalis n. sp., was isolated from body cavities burying beetles,...
Oxygen is required for the completion of almost all known metazoan lifecycles, but many metazoans harbour abilities to withstand varying degrees and periods hypoxia. Caenorhabditis elegans, one most popular model organism extensively used as a study hypoxia anoxia biology it has been found that this nematode capable tolerance Considering extremely high diversity nematodes, effects low oxygen concentration mechanisms adaptation depletion differ among species. In study, we simple assay examine...
Bursaphelenchus xylophilus has been destroying pine forests in East Asia and western Europe. Here, we report its nearly complete genomic sequence containing five ∼12-Mb scaffolds one ∼15-Mb scaffold representing six chromosomes. Large repeat regions that were previously unidentified are now reasonably integrated, particularly the scaffold.
We examined the nematode fauna associated with a species of bark beetle, Dryocoetes uniseriatus Eggers, as part biodiversity survey forest beetle-associated nematodes. Collections were made in pine stand at an experimental station Ibaraki, Japan, from April to July 2011; we association 273 insects collected during this time. In total, 68% least one nematode. Six nematodes, including two phoretic microbe feeders (Bursaphelenchus rainulfi Braasch & Burgermeister and Micoletzkya sp.), insect...
A Sheraphelenchus species was isolated from a sample of sap exuding scar on the bark Quercus serrata . Besides its generic characteristics, i.e. , posteriorly located vulva in female, male spicule with conspicuous dorsal limb and tail spike-like projection, new is characterised by short stylet small basal swelling. The near-full-length 18S D2/D3 expansion segments ribosomal RNA genes (near-full SSU D2/D3LSU) were determined as molecular barcode sequences phylogenetic status (= genus )...
We demonstrated the disembarkation of bacterial-feeding nematode Caenorhabditis japonica dauer larvae (DL) from adult Parastrachia japonensis female insects and observed propagation nematodes in artificial insect nests. Our results clarify process this species provide insights into nematode-insect relationship. Quiescent C. DL resumed their mobility only at > 99.9% relative humidity (RH) 25°C presence or absence carrier insect. In nests with RH, number on decreased gradually after...