A5009794361- Genetics, Aging, and Longevity in Model Organisms
- Parasite Biology and Host Interactions
- Genomics and Phylogenetic Studies
- Chromosomal and Genetic Variations
- Parasites and Host Interactions
- Nematode management and characterization studies
- Insect Resistance and Genetics
- Gut microbiota and health
- Legume Nitrogen Fixing Symbiosis
- Nitrogen and Sulfur Effects on Brassica
- Insect and Arachnid Ecology and Behavior
- Circadian rhythm and melatonin
- Evolution and Genetic Dynamics
- Diamond and Carbon-based Materials Research
- CRISPR and Genetic Engineering
- thermodynamics and calorimetric analyses
- Thermoregulation and physiological responses
- Biochemical Analysis and Sensing Techniques
- Neuroendocrine regulation and behavior
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Spaceflight effects on biology
- Yeasts and Rust Fungi Studies
- Plant Pathogens and Fungal Diseases
- Insect symbiosis and bacterial influences
- Mitochondrial Function and Pathology
Osaka City University
2016-2024
The University of Tokyo
2022-2024
University of Miyazaki
2020-2023
Taisei (Japan)
2022
Forestry and Forest Products Research Institute
2022
Düzce Üniversitesi
2022
Real-time temperature monitoring inside living organisms provides a direct measure of their biological activities, such as homeostatic thermoregulation and energy metabolism. However, it is challenging to reduce the size bio-compatible thermometers down submicrometers despite potential applications for thermal imaging subtissue structures with single-cell resolution. Light-emitting nanothermometers that remotely sense via optical signals exhibit considerable in \textit{in-vivo}...
Abstract Large-scale genome-structural evolution is common in various organisms. Recent developments speciation genomics revealed the importance of inversions, whereas role other rearrangements, including chromosome fusions, have not been well characterized. We study genomic divergence and reproductive isolation closely related nematodes: androdioecious (hermaphroditic) model Pristionchus pacificus its dioecious sister species exspectatus . A chromosome-level genome assembly P. using...
Abstract Sex determination mechanisms evolve surprisingly rapidly, yet little is known in the large nematode phylum other than for Caenorhabditis elegans , which relies on chromosomal XX-XO sex and a dosage compensation mechanism. Here we analyze by sex-specific genome sequencing genetic analysis two fungal feeding/plant-parasitic Bursaphelenchus nematodes find that their differentiation more likely triggered random, epigenetic regulation well-known of or environmental determination. There...
Clostridium butyricum MIYAIRI 588 (CBM 588), one of the probiotic bacterial strains used for humans and domestic animals, has been reported to exert a variety beneficial health effects. The effect this on lifespan, however, is unknown. In present study, we investigated CBM lifespan multiple-stress resistance using Caenorhabditis elegans as model animal. When adult C. were fed standard diet Escherichia coli OP50 or 588, animals was significantly longer than that OP50. addition, CBM588...
The Caenorhabditis elegans ( C. ) amphid sensory organ contains only 4 glia‐like cells and 24 neurons, providing a simple model for analyzing glia or neuron‐glia interactions. To better characterize glial development function, we carried out RNA interference screening transcription factors that regulate the expression of an sheath cell marker identified pros‐1 , which encodes homeodomain factor homologous to Drosophila prospero /mammalian Prox1 as positive regulator. functional PROS‐1::EGFP...
Bursaphelenchus xylophilus has been destroying pine forests in East Asia and western Europe. Here, we report its nearly complete genomic sequence containing five ∼12-Mb scaffolds one ∼15-Mb scaffold representing six chromosomes. Large repeat regions that were previously unidentified are now reasonably integrated, particularly the scaffold.
The factors contributing to evolution of androdioecy, the coexistence hermaphrodites and males such as in Caenorhabditis elegans, remains poorly known. However, nematodes exhibit androdioecy at last 13 genera with predatory genus Pristionchus having seven independent transitions towards androdioecy. Nonetheless, associated genomic architecture sex determination mechanisms are largely known from Caenorhabditis. Here, studying 47 species, we observed repeated chromosome which abolished...
Abstract The cryptic parasite Sparganum proliferum proliferates in humans and invades tissues organs. Only scattered cases have been reported, but S. infection is always fatal. However, ’s phylogeny life cycle remain enigmatic. To investigate the phylogenetic relationships between other cestode species, to examine mechanisms underlying pathogenicity, we sequenced entire genomes of a closely related non–life-threatening tapeworm Spirometra erinaceieuropaei . Additionally, performed larvae...
The Strongyloides clade, to which the parasitic nematode genus belongs, contains taxa with diverse lifestyles, ranging from free-living obligate vertebrate parasites. Reproductive strategies are also in this group of nematodes, employing not only sexual reproduction but parthenogenesis, making it an attractive study genome adaptation specific conditions. An in-depth understanding evolution, however, has been hampered by fragmented assemblies. In study, we generated chromosome-level...
Bifidobacterium infantis, a Gram-positive bacterium, is one of the commonly used probiotics. We previously showed that B. infantis modified host defense systems and extended lifespan nematode Caenorhabditis elegans. In present study, we extension caused by was enhanced in animals having mutation tol-1 gene encodes sole C. elegans homolog Toll-like receptors (TLRs). Meanwhile, increased other probiotic bacteria, such as Bacillus subtilis or Clostridium butyricum, not affected mutant animals....
Abstract Caenorhabditis auriculariae, which was morphologically described in 1999, re-isolated from a Platydema mushroom-associated beetle. Based on the materials, some morphological characteristics were re-examined and ascribed to species. In addition, clarify phylogenetic relationships with other species biological features of nematode, whole genome sequenced assembled into 109.5 Mb 16,279 predicted protein-coding genes. Molecular analyses based ribosomal RNA 269 single-copy genes revealed...
Abstract Background The first metazoan genome sequenced, that of Caenorhabditis elegans , has motivated animal evolution studies. To date > 50 species from the genus have been allowing research on variation. Results In present study, we describe a new gonochoristic species, niphades n. sp., previously referred as C. sp. 36, isolated adult weevils ( Niphades variegatus ), with whom they appear to be tightly associated during its life cycle. Along description, sequenced and produced...
Bursaphelenchus okinawaensis is a self-fertilizing, hermaphroditic, fungus-feeding nematode used as laboratory model for the genus Bursaphelenchus, which includes important pathogen xylophilus Here, we report nearly complete genome sequence of B. The 70-Mbp assembly contained six scaffolds (>11 Mbp each) with telomere repeats on their ends, indicating chromosomes.
Aims This study aimed to investigate the effect of Bacillus subtilis var. natto on susceptibility model host, Caenorhabditis elegans, bacterial infection. Methods and Results elegans worms were fed with a standard food consisting Escherichia coli OP50 strain (control) or B. (natto) during their larval stage. The then infected pathogenic bacteria. We analyzed survival time RNA sequencing-based transcriptome. Upon infection Staphylococcus aureus Enterococcus faecalis, (natto)-fed was longer...
Bitterness and astringency are the aversive tastes in mammals. In humans, aversion to bitterness may be reduced depending on eating experience. However, cellular molecular mechanisms underlying plasticity preference bitter astringent tastants remain unknown. This study aimed investigate tea polyphenols, including catechins tannic acids, model animal Caenorhabditis elegans. C. elegans showed avoidance behavior against epigallocatechin gallate (EGCG), acid, theaflavin. they displayed...
The transition of the sexual mode occurs widely in animal evolution. In Caenorhabditis nematodes, androdioecy, a polymorphism composed males and hermaphrodites having ability to self-fertilize, has evolved independently multiple times. While modification noncoding regulatory elements likely contributed evolution hermaphroditism, little is known about these changes. Here, we conducted genome-wide analysis conserved (CNEs) focusing on hermaphroditism nematodes. We found that, androdioecious...
ABSTRACT Endoderm specification in Caenorhabditis elegans occurs through a network which maternally provided SKN-1/Nrf, with additional input from POP-1/TCF, activates the GATA factor cascade MED-1,2→END-1,3→ELT-2,7. Orthologues of MED, END and ELT-7 factors are found only among nematodes closely related to C. elegans, raising question how gut is specified their absence more distant species genus. We find that angaria, portoensis monodelphis orthologues gene elt-3 expressed early E lineage,...
The membrane trafficking events that regulate unicellular tube formation and maintenance are not well understood. Here, using an RNAi screen, we identified the small GTPase ARF1 homolog ARF-1.2 as a regulator of excretory in Caenorhabditis elegans. RNAi-mediated knockdown knockout arf-1.2 gene resulted large intracellular vacuoles at growth sites (varicosities) canals. mutant animals were sensitive to hyperosmotic conditions. affected localization anion transporter SULP-8, which is expressed...
Real-time temperature monitoring inside living organisms provides a direct measure of their biological activities, such as homeostatic thermoregulation and energy metabolism. Here, using quantum nanothermometers based on optically accessible electron spins nitrogen vacancy centers in nanodiamonds, we demonstrate vivo real-time nematode worms. We developed thermometry system that can the temperatures mobile nanodiamonds worms with precision ± 0.22 oC. Using this system, determined increase...