A5015984192- Escherichia coli research studies
- Viral gastroenteritis research and epidemiology
- Salmonella and Campylobacter epidemiology
- Genetics, Aging, and Longevity in Model Organisms
- Neural Networks and Applications
- Vibrio bacteria research studies
- Listeria monocytogenes in Food Safety
- Advanced Control Systems Optimization
- Metaheuristic Optimization Algorithms Research
- Smart Grid Energy Management
- Evolutionary Algorithms and Applications
- Scheduling and Optimization Algorithms
- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Advanced Multi-Objective Optimization Algorithms
- Visual perception and processing mechanisms
- Probiotics and Fermented Foods
- Aquaculture disease management and microbiota
- Advanced Manufacturing and Logistics Optimization
- Power Systems and Renewable Energy
- Water Systems and Optimization
- Enterobacteriaceae and Cronobacter Research
- Circadian rhythm and melatonin
- Enzyme Structure and Function
- Bacteriophages and microbial interactions
Tezukayama Gakuin University
2020-2025
Osaka City University
2014-2023
Kobe University
2011
Kyoto University
1991-2006
Tokyo Metropolitan University
2004-2006
Osaka Institute of Technology
1996-2003
Osaka Prefectural Institute of Public Health
1993-2003
Fukuoka Institute of Technology
2003
Mitsubishi Group (Japan)
2003
Changchun University
2002
This study aimed to develop a convenient model investigate the senescence of host defenses and influence food nutrition. A small soil nematode, Caenorhabditis elegans, was grown for 3 days from hatching on lawn Escherichia coli OP50 as normal source, subsequently some nematodes were fed lactic acid bacteria (LAB). The life spans worms LAB significantly longer than those OP50. To effect age defenses, 3- 7-day-old transferred onto Salmonella enterica serovar Enteritidis infection. died over...
In Parkinson's disease (PD), α–synuclein (αSyn) accumulation drives neuropathological progression, establishing it as a potential therapeutic target. Lactic acid bacteria (LAB) provide various health benefits to the host and are expected offer protective effects on neurological functions through brain–gut connection, indicated by animal studies. However, of LAB against αSyn not well understood. We investigated whether feeding could reduce improve mobility in transgenic Caenorhabditis elegans...
ABSTRACT The pathogenesis of enterotoxigenic Escherichia coli (ETEC) involves the colonization hosts by factors (CFs) and secretion enterotoxins. CFs, especially chaperone‐usher fimbriae, mediate bacterial adhesion to host cells, with extensive genetic diversity observed among isolates. One ETEC strain, O169YN10, possessed a unique plasmid (pEntYN10) encoding three CS6, two novel homologs CS8 F4 (CS6 O169 , ). In this study, was found play major role in multiple hosts, including human,...
From July 1985 to March 1987, the occurrence of motile Aeromonas sp. in stool, food and environmental specimens was investigated assess their pathogenic significance determine sources routes infection. A total 9366 stool were examined; isolated from 11.1% diarrhoeal stools 2.2% normal (P less than 0.001). counts specimens, which included minced beef, pork chicken, seafood various vegetables products, unexpectedly high suggesting that infection might be food-borne rather water-borne. About...
Abstract To assess the utility of autofluorescence as a noninvasive biomarker senescence in Caenorhabditis elegans , we measured individual nematodes using spectrofluorometry. The fluorescence each worm increased with age. Animals lower intensity exhibited longer life expectancy. When proteins extracted from worms were incubated sugars, and concentration advanced glycation end products (AGEs) over time. Ribose enhanced these changes not only vitro but also vivo. blocker rifampicin suppressed...
Aims: To determine the prevalence and molecular characteristics of Shiga toxin-producing Escherichia coli (STEC) enteropathogenic E. (EPEC) in calves lambs with diarrhoea India. Methods Results: Faecal samples originating from 391 101 which had were screened for presence coli. A total number 309 (249 bovine 60 ovine) strains isolated. 113 15 ovine subjected to multiplex polymerase chain reaction (m-PCR) detection stx1, stx2, eaeA EHEC hlyA genes. STEC EPEC belonging different serogpoups...
To investigate the presence or absence of shiga toxin-producing Escherichia coli (STEC) in avian species India.Faecal samples originating from 500 chicken and 25 free flying pigeons were screened for E. coli. A total 426 (chicken, 401; pigeons, 25) strains isolated. Of strains, 387 grouped into 77 serogroups, while 70 59 untypable rough, respectively. All isolates subjected to multiplex polymerase chain reaction (m-PCR) detection stx(1), stx(2), eaeA, hlyA saa genes. None studied showed...
In an outbreak of gastroenteritis on 23 July 1996, in Osaka, Japan, 54 91 persons who had attended a meeting the previous day became ill. Escherichia coli O166:H15 was isolated from stool specimens patients (29/33, 88%). Laboratory tests for other bacterial pathogens and viruses were negative. The E. 0166 organisms did not adhere to HEp-2 cells localized, diffuse, or enteroaggregative manner. express known enterotoxigenic (ETEC) colonization factors. polymerase chain reaction tests, bacteria...
The survival times of Caenorhabditis elegans worms infected with Legionella pneumophila from day 7.5 or later after hatching were shorter than those uninfected worms. However, nematodes fed bifidobacteria prior to infection resistant Legionella. These may act as a unique alternative host for research.
Aims: The source and routes of diarrhoeagenic Escherichia coli (DEC) have not been clarified because it is difficult to detect these organisms in samples with numerous coliform bacteria. We developed multiplex real-time PCR assays for exhaustive detection DEC. Methods Results: Primers TaqMan probes were designed amplify quantify one gene (eae, stx1, stx2, elt, est, virB, aggR, astA, afaB) from each seven pathotypes DEC, duplex or triplex reactions under the same cycling conditions....
Clostridium butyricum MIYAIRI 588 (CBM 588), one of the probiotic bacterial strains used for humans and domestic animals, has been reported to exert a variety beneficial health effects. The effect this on lifespan, however, is unknown. In present study, we investigated CBM lifespan multiple-stress resistance using Caenorhabditis elegans as model animal. When adult C. were fed standard diet Escherichia coli OP50 or 588, animals was significantly longer than that OP50. addition, CBM588...
Lactic acid bacteria are beneficial to Caenorhabditis elegans; however, acting as probiotics in nematodes may not necessarily have probiotic functions humans. Lactococcus cremoris subsp. reportedly has Therefore, we determined whether the strain FC could exert effects C. elegans terms of improving host defenses and extending life span. Live successfully extended span enhanced defense compared Escherichia coli OP50 (OP50), a standard food source for elegans. The FC-fed worms were tolerant...