A5072772526- Genetics, Aging, and Longevity in Model Organisms
- CRISPR and Genetic Engineering
- Mitochondrial Function and Pathology
- Circadian rhythm and melatonin
- Endoplasmic Reticulum Stress and Disease
- Cellular transport and secretion
- RNA Research and Splicing
- Pancreatic function and diabetes
- Spaceflight effects on biology
- Phagocytosis and Immune Regulation
- Pluripotent Stem Cells Research
- DNA Repair Mechanisms
- Alzheimer's disease research and treatments
- Cell death mechanisms and regulation
- Lysosomal Storage Disorders Research
- Birth, Development, and Health
- Lipid metabolism and biosynthesis
- Autophagy in Disease and Therapy
- Animal Genetics and Reproduction
- RNA regulation and disease
- Viral Infectious Diseases and Gene Expression in Insects
- Proteoglycans and glycosaminoglycans research
- Muscle Physiology and Disorders
- Microtubule and mitosis dynamics
- Reproductive Biology and Fertility
Tokyo Women's Medical University
2015-2024
Nippon Dental University
2024
Kyoto University
2023
NEC (Japan)
2021
Japan Science and Technology Agency
2009-2020
University of Miyazaki
2013
Kyushu University
2006
Centre for Research in Engineering Surface Technology
2006
Center for Responsible Travel
2006
Centre de Recherche en Économie et Statistique
2006
Mitochondria are inherited maternally in most animals, but the mechanisms of selective paternal mitochondrial elimination (PME) unknown. While examining fertilization Caenorhabditis elegans, we observed that mitochondria rapidly lose their inner membrane integrity. CPS-6, a endonuclease G, serves as factor is critical for PME. We found CPS-6 relocates from intermembrane space to matrix after degrade DNA. It acts with maternal autophagy and proteasome machineries promote Loss cps-6 delays...
During apoptosis, phosphatidylserine, which is normally restricted to the inner leaflet of plasma membrane, exposed on surface apoptotic cells and has been suggested act as an "eat-me" signal trigger phagocytosis. It unclear how phagocytes recognize phosphatidylserine. Recently, a putative phosphatidylserine receptor (PSR) was identified proposed mediate recognition We report that psr-1, Caenorhabditis elegans homolog PSR, important for cell corpse engulfment. In vitro PSR-1 binds...
Mutations in alpha-synuclein gene cause familial form of Parkinson disease, and deposition wild-type as Lewy bodies occurs a hallmark lesion sporadic disease dementia with bodies, implicating the pathogenesis related neurodegenerative diseases. Dopamine neurons substantia nigra are major site neurodegeneration associated disease. Here we establish transgenic Caenorhabditis elegans (TG worms) that overexpresses or mutant human dopamine neurons. The TG worms exhibit accumulation cell neurites...
Mutations or multiplications in α-synuclein gene cause familial forms of Parkinson disease dementia with Lewy bodies (LB), and the deposition wild-type as LB occurs a hallmark lesion these disorders, collectively referred to synucleinopathies, implicating pathogenesis synucleinopathy. To identify modifier genes α-synuclein-induced neurotoxicity, we conducted an RNAi screen transgenic C . elegans (Tg worms) that overexpress human pan-neuronal manner. enhance effect neurons, crossed Tg worms...
Small RNAs regulate diverse biological processes by directing effector proteins called Argonautes to silence complementary mRNAs. Maturation of some classes small involves terminal 2'-O-methylation prevent degradation. This modification is catalyzed members the conserved HEN1 RNA methyltransferase family. In animals, Piwi-interacting (piRNAs) and endogenous exogenous interfering (siRNAs) are methylated, whereas microRNAs not. However, mechanisms that determine animal substrate specificity...
Abstract Background Transgenic strains of Caenorhabditis elegans are typically generated by injecting DNA into the germline to form multi-copy extrachromosomal arrays. These transgenes semi-stable and their expression is silenced in germline. Mos1 transposon or microparticle bombardment methods have been developed create single- low-copy chromosomal integrated lines. Here we report an alternative method using ultraviolet trimethylpsoralen (UV/TMP) generate single/low-copy gene integrations....
Balancer chromosomes are critical tools for genetic research. In C. elegans, reciprocal translocations that lead to aneuploidy have been widely used maintain lethal and sterile mutations in stable stocks. Here, we generated a set of aneuploidy-free structurally defined crossover suppressors contain two overlapping inversions using the CRISPR/Cas9 system. The toolkit includes 13 covers approximately 63% all elegans coding genes. Together with classical intrachromosomal suppressors, system now...
Phosphatidylinositol 3-phosphate (PtdIns3P) plays a central role in endosome fusion, recycling, sorting, and early-to-late conversion, but the mechanisms that determine how correct endosomal PtdIns3P level is achieved remain largely elusive. Here we identify two new factors, SORF-1 SORF-2, as essential regulators Caenorhabditis elegans. Loss of sorf-1 or sorf-2 leads to greatly elevated PtdIns3P, which drives excessive fusion early endosomes. function coordinately with Rab switching genes...
ABSTRACT Six touch receptor neurons with distinctive morphological features sense gentle in Caenorhabditis elegans. Previous studies have identified three genes (lin-32, unc-86 and mec-3) that regulate cell development. However, since other types also require these genes, we suspected help restrict the expression of characteristics to six seen wild type. To identify such examined mutants defective required for development C. elegans cells changes pattern cell-specific features. Mutations...
The asymmetrical distribution of phospholipids on the plasma membrane is critical for maintaining cell integrity and physiology regulating intracellular signaling important cellular events such as clearance apoptotic cells. How phospholipid asymmetry established maintained not fully understood. We report that Caenorhabditis elegans P-type adenosine triphosphatase homolog, TAT-1, surface phosphatidylserine (PS). In animals deficient in tat-1 , PS abnormally exposed surface, normally living...
Phosphatidylinositol (PI) is a component of membrane phospholipids, and it functions both as signaling molecule compartment-specific localization signal in the form polyphosphoinositides. Arachidonic acid (AA) predominant fatty sn-2 position PI mammals. LysoPI acyltransferase (LPIAT) thought to catalyze formation AA-containing PI; however, gene that encodes this enzyme has not yet been identified. In study, we established screening system identify genes required for use exogenous...