A5009492244- Calcium signaling and nucleotide metabolism
- Galectins and Cancer Biology
- Lysosomal Storage Disorders Research
- Autophagy in Disease and Therapy
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer, Hypoxia, and Metabolism
- ATP Synthase and ATPases Research
- Phagocytosis and Immune Regulation
- Cellular transport and secretion
- Genomics and Phylogenetic Studies
- Ubiquitin and proteasome pathways
- Cellular Mechanics and Interactions
- Adenosine and Purinergic Signaling
- Cell death mechanisms and regulation
- Ferroptosis and cancer prognosis
- Metabolomics and Mass Spectrometry Studies
- Biological Activity of Diterpenoids and Biflavonoids
- Epigenetics and DNA Methylation
- Cancer, Lipids, and Metabolism
- Genetic Mapping and Diversity in Plants and Animals
- RNA modifications and cancer
- Synthesis of Organic Compounds
- Chromosomal and Genetic Variations
- Immune cells in cancer
- Microtubule and mitosis dynamics
Ningxia Medical University
2023-2025
Danish Cancer Society
2015-2024
Cancer Institute (WIA)
2024
Qinghai Provincial Peoples Hospital
2023-2024
Hunan University
2023
Bengbu Medical College
2023
Beijing University of Chinese Medicine
2022
Kyoto University
2017
XinHua Hospital
2017
University of Benin
2016
We have produced a draft sequence of the rice genome for most widely cultivated subspecies in China, Oryza sativa L. ssp. indica , by whole-genome shotgun sequencing. The was 466 megabases size, with an estimated 46,022 to 55,615 genes. Functional coverage assembled sequences 92.0%. About 42.2% exact 20-nucleotide oligomer repeats, and transposons were intergenic regions between Although 80.6% predicted Arabidopsis thaliana genes had homolog rice, only 49.4% A. . large proportion no...
We report a draft sequence for the genome of domesticated silkworm (Bombyx mori), covering 90.9% all known genes. Our estimated gene count is 18,510, which exceeds 13,379 genes reported Drosophila melanogaster. Comparative analyses to fruitfly, mosquito, spider, and butterfly reveal both similarities differences in content.
Lysosomal membrane permeabilization (LMP) contributes to tissue involution, degenerative diseases, and cancer therapy. Its investigation has, however, been hindered by the lack of sensitive methods. Here, we characterize validate detection galectin puncta at leaky lysosomes as a highly easily manageable assay for LMP. LGALS1/galectin-1 LGALS3/galectin-3 are best suited this purpose due their widespread expression, rapid translocation availability high-affinity antibodies. Galectin staining...
Abstract Background Comparative whole genome analysis of Mammalia can benefit from the addition more species. The pig is an obvious choice due to its economic and medical importance as well evolutionary position in artiodactyls. Results We have generated ~3.84 million shotgun sequences (0.66X coverage) genome. data are hereby released (NCBI Trace repository with center name "SDJVP", project "Sino-Danish Pig Genome Project") together initial analysis. non-repetitive fraction was aligned UCSC...
Defective catabolite export from lysosomes results in lysosomal storage diseases humans. Mutations the cystine transporter gene CTNS cause cystinosis, but other amino acid transporters are poorly characterized at molecular level. Here, we identified Caenorhabditis elegans lysine/arginine LAAT-1. Loss of laat-1 caused accumulation lysine and arginine enlarged, degradation-defective lysosomes. In mutants ctns-1 (C. homolog CTNS), LAAT-1 was required to reduce levels suppress lysosome...
Magnetic nanoparticles (MNPs) functionalized with targeting moieties can recognize specific cell components and induce mechanical actuation under magnetic field.Their size is adequate for reaching tumors cancer cells.However, due to the nanometric size, force generated by MNPs smaller than required largely disrupting key of cells.Here, we show assembly process inside cells, form elongated aggregates produce elevated forces.We synthesized iron oxide doped zinc, obtain high magnetization,...
The autophagic clearance of damaged lysosomes by lysophagy involves extensive modification the organelle with ubiquitin, but underlying ubiquitination machinery is still poorly characterized. Here, we use an siRNA screening approach and identify human UBE2QL1 as a major regulator lysosomal ubiquitination, lysophagy, cell survival after damage. translocates to permeabilized where it associates damage sensors, targets, effectors. knockdown reduces accumulation critical autophagy receptor p62...
Dysregulated intracellular pH is emerging as a hallmark of cancer. In spite their acidic environment and increased acid production, cancer cells maintain alkaline that promotes progression by inhibiting apoptosis increasing glycolysis, cell growth, migration, invasion. Here we identify signal transducer activator transcription-3 (STAT3) key factor in the preservation cytosol. STAT3 associates with vacuolar H+-ATPase coiled-coil domain-dependent manner increases its activity living vitro....
Abstract The accumulation of senescent cells is recognised as a driver tissue and organismal ageing. One the gold‐standard hallmarks cell an increase in lysosomal content, measured by senescence‐associated β‐galactosidase (Senβ‐Gal) activity. lysosome plays central role integrating mitogenic stress cues to control metabolism, which known be dysregulated senescence. Despite this, little about cause consequence biogenesis We find here that lysosomes are dysfunctional; they have higher pH,...
Abstract Lysosomes are membrane-surrounded cytoplasmic organelles filled with a powerful cocktail of hydrolases. Besides degrading cellular constituents inside the lysosomal lumen, hydrolases promote tissue remodeling when delivered to extracellular space and cell death released cytosol. Here, we show that spatially temporally controlled leakage contributes accurate chromosome segregation in normal mammalian division. One or more chromatin-proximal lysosomes leak majority prometaphases,...
Cancer cells generate large quantities of cytoplasmic protons as byproducts aberrantly activated aerobic glycolysis and lactate fermentation. To avoid potentially detrimental acidification the intracellular milieu, cancer activate multiple acid-removal pathways that promote cytosolic alkalization extracellular acidification. Accumulating evidence suggests in addition to well-characterized ion pumps exchangers plasma membrane, cell lysosomes are also reprogrammed for this purpose. On one...
Abstract The mechanisms linking tumor microenvironment acidosis to disease progression are not understood. Here, we used mammary, pancreatic, and colon cancer cells show that adaptation growth at an extracellular pH (pH e ) mimicking acidic niches is associated with upregulated net acid extrusion capacity elevated intracellular physiological , but . Using metabolic profiling, shotgun lipidomics, imaging biochemical analyses, the adaptation‐induced phenotype characterized by a shift toward...
Repurposing cationic amphiphilic drugs (CAD) for cancer treatment is emerging as an attractive means to enhance the efficacy of chemotherapy. Many commonly used CADs, including several cation antihistamines and antidepressants, induce cancer-specific, lysosome-dependent cell death sensitize cells CAD-induced inhibition lysosomal acid sphingomyelinase necessary, but not sufficient, subsequent membrane permeabilization death, while other pathways regulating this pathway are largely unknown....
Commonly used antihistamines and other cationic amphiphilic drugs (CADs) are emerging as putative cancer drugs. Their unique chemical structure enables CADs to accumulate rapidly inside lysosomes, where they increase lysosomal pH, alter lipid metabolism, eventually cause membrane permeabilization. Here, we show that CAD-induced rapid elevation in pH is caused by a H+ efflux requires P2RX4-mediated Ca2+ release precedes the The subsequent cytosolic acidification triggers dephosphorylation,...
The acidic pH of the tumor microenvironment plays a critical role in driving cancer development toward more aggressive phenotype, but underlying mechanisms are unclear. To this end, phenotypic and genotypic changes induced by adaptation cells to chronic acidosis have been studied. However, generality acid patterns across cell models their correlation molecular phenotypes aggressiveness human cancers essentially unknown. Here, we define an expression response shared three models, dominated...
Abstract Cancer cells are dependent on cholesterol, and they possess strictly controlled cholesterol homeostasis mechanisms. These allow them to smoothly switch between synthesis uptake fulfill their needs adapt environmental changes. Here we describe a mechanism of how cancer employ oncogenic growth factor signaling promote utilization extracellular via Myeloid Zinc Finger 1 (MZF1)-mediated Niemann Pick C1 (NPC1) expression upregulated macropinocytosis. Expression p95ErbB2, highly...
Atherosclerosis is chronic inflammatory disease tightly related with defective efferocytosis of apoptotic cells (ACs) in the plaques. The strategy reactivation using Atorvastatin (AT) and Metformin (Met) has attracted increasing attention for atherosclerosis treatment. Although these drug-based therapies could stimulate phagocytic clearance ACs, rapid drug poor bioavailability limited their efficacy. In this study, by co-loading AT Met into poly(lactic-co-glycolic) acid nanoparticles (PLGA...
Working in parallel with the efforts to sequence chicken (Gallus gallus) genome, Beijing Genomics Institute led an international team of scientists from China, USA, UK, Sweden, The Netherlands and Germany map extensive DNA variation throughout genome by sampling domestic breeds.Using Red Jungle Fowl as a reference, we identified 3.1 million non-redundant variants.To facilitate application our data avian genetics provide foundation for functional evolutionary studies, created 'Chicken...
Abstract The transcription factor cyclic-AMP response element-binding protein 1 (CREB1) responds to cAMP level and controls the expression of target genes, which regulates nutrition partitioning. promoters CREB1-targeted genes responsive have been extensively investigated characterized with presence both element TATA box. Compelling evidence demonstrates that CREB1 also plays an essential role in promoting tumor development. However, only very few required for cell survival, proliferation...