A5079596808- Antibiotic Resistance in Bacteria
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Bacterial biofilms and quorum sensing
- Antimicrobial Resistance in Staphylococcus
- Enterobacteriaceae and Cronobacter Research
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Microbial Community Ecology and Physiology
- Microbial infections and disease research
- Probiotics and Fermented Foods
- Microbial Metabolism and Applications
- Bacterial Identification and Susceptibility Testing
- Algal biology and biofuel production
- Photosynthetic Processes and Mechanisms
- Hormonal Regulation and Hypertension
- Protist diversity and phylogeny
- Plant Pathogenic Bacteria Studies
- Pediatric health and respiratory diseases
- Evolution and Genetic Dynamics
- Aquaculture disease management and microbiota
- Vibrio bacteria research studies
- Salmonella and Campylobacter epidemiology
- Animal Virus Infections Studies
- SARS-CoV-2 and COVID-19 Research
Zhejiang University
2003-2024
Wenzhou Medical University
2014-2023
Qingdao Agricultural University
2023
Xinqiao Hospital
2022
Army Medical University
2022
Life Science Institute
2015-2020
Zhejiang Taizhou Hospital
2020
Ministry of Education of the People's Republic of China
2019
Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University
2018
BGI Group (China)
2002-2016
We have produced a draft sequence of the rice genome for most widely cultivated subspecies in China, Oryza sativa L. ssp. indica , by whole-genome shotgun sequencing. The was 466 megabases size, with an estimated 46,022 to 55,615 genes. Functional coverage assembled sequences 92.0%. About 42.2% exact 20-nucleotide oligomer repeats, and transposons were intergenic regions between Although 80.6% predicted Arabidopsis thaliana genes had homolog rice, only 49.4% A. . large proportion no...
Bacterial resistance to the antibiotics has become an important concern for public health. This study was aimed investigate characteristics and distribution of florfenicol-related genes in bacteria isolated from 4 farms. A total 106 florfenicol-resistant Gram-negative bacilli were examined positive isolates further characterized. Antimicrobial sensitivity results showed that most them (100, 94.33%) belonged multidrug Enterobacteriaceae. About 91.51% strains carried floR gene, 4.72% cfr gene....
Staphylococcus aureus colonization and infection occur more commonly among persons living or working in crowded conditions, but characterization of S. within medical communities China is lacking. A total 144 (15.4%, 144/935) isolates, including 28 (3.0%, 28/935) MRSA were recovered from the nares 935 healthy human volunteers residing on a Chinese college campus. All isolates susceptible to vancomycin, quinupristin/dalfopristin linezolid majority resistant penicillin (96.5%),...
Florfenicol is a derivative of chloramphenicol that used only for the treatment animal diseases. A key resistance gene florfenicol, floR, can spread among bacteria same and different species or genera through horizontal transfer. To analyze potential transmission genes between human pathogens, we investigated floR in Klebsiella pneumoniae isolates from patient samples. pathogens may originate would reflect risk to health using antimicrobial agents animals.PCR was identify floR-positive...
Arthrospira (Spirulina) platensis as a representative species of cyanobacteria has been recognized and used worldwide source protein in the food, which possesses some unusual valuable physiological characteristics, such alkali salt tolerance. Based on complete genome sequencing plantensis-YZ, we compared expression profiles this organism under different salt-stress conditions (i.e. 0.02 M, 0.5 M 1.0 NaCl, respectively), using 2-D electrophoresis peptide mass fingerprinting, retrieved 141...
Arthrospira platensis is a multi-cellular and filamentous non-N2-fixing cyanobacterium that capable of performing oxygenic photosynthesis. In this study, we determined the nearly complete genome sequence A. YZ. YZ single, circular chromosome 6.62 Mb in size. Phylogenetic comparative genomic analyses revealed was more closely related to NIES-39 than sp. PCC 8005 C1. Broad gene gains were identified between three other speices, some which have been previously demonstrated can be laterally...
Abstract Background With the wide use of florfenicol to prevent and treat bacterial infection domestic animals, emergence resistance bacteria is increasingly serious. It very important elucidate molecular mechanism bacteria’s florfenicol. Methods The minimum inhibitory concentration (MIC) levels were determined by agar dilution method, polymerase chain reaction was conducted analyze distribution genes in 39 CoNS strains isolated from poultry livestock animals seafood. whole genome sequence...
Klebsiella pneumoniae is a clinically significant species of bacterium which causes variety diseases. Clinical treatment this bacterial infection greatly hindered by the emergence multidrug-resistant strains. The resistance largely due to acquisition plasmids carrying drug-resistant as well pathogenic genes, and its conjugal transfer facilitates spread resistant phenotypes.The 70,057 bp plasmid pKF3-70, commonly found in pneumoniae, composed five main functional modules, including regions...
To investigate the mechanisms of multiple resistance and horizontal transfer genes in animal pathogens, we characterized molecular structures gene-related sequences a multidrug-resistant Klebsiella pneumoniae strain R46 isolated from rabbit. Molecular cloning was performed to clone genes, minimum inhibitory concentrations (MICs) were measured determine characteristics cloned related strains. A conjugation experiment conducted assess transferability plasmids. Sequencing comparative genomic...
Staphylococcus caprae, capitis and epidermidis belong to the "Epidermidis Cluster Group" (ECG) are generally opportunistic pathogens. In this work, whole genome sequencing, molecular cloning pan-genome analysis were performed investigate genetic characteristics of resistance, virulence structures 69 ECG strains, including a clinical isolate (S. caprae SY333) obtained in work. Two resistance genes (blaZ aadD2) encoded on plasmids pSY333-41 pSY333-45 S. SY333 confirmed be functional. The bla...
A total of 514 consecutive clinical Escherichia coli isolates, irrespective resistance background, were collected in the period 2002-2008 Wenzhou, southern China, to investigate prevalence plasmid-mediated quinolone (PMQR). The dominant PMQR gene was aac(6')-Ib-cr, followed by qnr, whereas qepA absent. 253 (49.2%) these isolates aac(6')-Ib-positive. Subsequently, 134 sequenced and 42 (31.3%) found harbor 18 new aac(6')-Ib mutants, 74 wild-type aac(6')-Ib. genes qnrA, qnrB, qnrS 2 (0.4%), 6...
Carbapenem resistance in Enterobacteriaceae is increasing and has become a matter of great concern. The aim this study was to characterize carbapenem-non-susceptible from teaching hospital. A total 49 clinical isolates recovered 2007–2010 the First Affiliated Hospital Wenzhou Medical College were analyzed by antimicrobial susceptibility testing. carbapenemase phenotype, outer membrane protein profiles, clonal relatedness investigated using modified Hodge test, sodium dodecyl...
In this work, by high-throughput sequencing, antibiotic resistance genes, including class A ( bla CTX-M , Z TEM VEB KLUC and SFO ), C SHV DHA MIR AZECL-29 ACT D OXA ) β -lactamase were identified among the pooled genomic DNA from 212 clinical Enterobacter cloacae isolates. Six -positive E. strains identified, pulsed-field gel electrophoresis (PFGE) showed that these not clonally related. The complete genome of - positive strain (Y546) consisted both a chromosome (4.78 Mb) large plasmid pY546...
The aim of this study was to analyze the molecular epidemiologic characteristics Acinetobacter baumannii. A total 398 isolates were collected in 7 regions South China from January June 2012. Drug sensitivity tested toward 15 commonly used antibiotics; thus, 146 multi-drug-resistant strains (resistant more than drugs) identified, representing 36.7% all isolates. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) for subtyping. According PFGE results (with a cutoff...
Due to inappropriate use, florfenicol resistance is becoming increasingly serious among animal respiratory tract and gut bacteria. To detect the mechanism Enterobacteriaceae bacteria, 292 isolates from feces were examined. The agar dilution method was conducted determine minimum inhibitory concentration (MIC) for florfenicol, polymerase chain reaction (PCR) performed genes. further explore molecular of resistance, whole-genome Leclercia adecarboxylata R25 sequenced. Of strains tested, 61.6%...
Bromodomain (BRD) proteins exhibit a variety of activities, such as histone modification, transcription factor recruitment, chromatin remodeling, and mediator or enhancer complex assembly, that affect initiation elongation. These also participate in epigenetic regulation. Although specific regulation plays an important role the occurrence development cancer, characteristics BRD family renal clear cell carcinoma (KIRC) have not been determined. In this study, we investigated expression genes...
This study was designed to characterize the dissemination mechanism and genetic context of Klebsiella pneumoniae carbapenemase (KPC) genes in carbapenem-resistant (CRKP) isolates.A retrospective analysis performed on CRKP strains isolated from a teaching hospital Wenzhou Medical University during 2015-2017. Polymerase chain reaction (PCR)-based amplification whole-genome sequencing (WGS) were used analyze blaKPC-2 gene. Conjugation experiments evaluate transferability blaKPC-2-bearing...
Florfenicol is widely used for the treatment of bacterial infections in domestic animals. The aim this study was to analyze molecular mechanisms florfenicol and oxazolidinone resistance Enterococcus isolates from anal feces minimum inhibitory concentration (MIC) levels were determined by agar dilution method. Polymerase chain reaction (PCR) performed distribution genes. Whole-genome sequencing comparative plasmid analysis conducted gene environment. A total 351 non-duplicated enteric strains...