A5039546638- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Bacteriophages and microbial interactions
- Salmonella and Campylobacter epidemiology
- Aquaculture disease management and microbiota
- RNA Interference and Gene Delivery
- Enterobacteriaceae and Cronobacter Research
- Satellite Image Processing and Photogrammetry
- Viral Infectious Diseases and Gene Expression in Insects
- Luminescence and Fluorescent Materials
- Microbial Metabolism and Applications
- Vibrio bacteria research studies
- Extremum Seeking Control Systems
- Heavy Metal Exposure and Toxicity
- Bacterial Genetics and Biotechnology
- Plant Pathogenic Bacteria Studies
- Blood properties and coagulation
- Nanoplatforms for cancer theranostics
- Phytase and its Applications
- Traditional Chinese Medicine Analysis
- Child Nutrition and Feeding Issues
- Chromium effects and bioremediation
- Liver Disease Diagnosis and Treatment
- Infections and bacterial resistance
- Neonatal Respiratory Health Research
Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University
2012-2025
Wenzhou Medical University
2014-2025
Southwest Petroleum University
2022
Phototherapy is an effective strategy to control Candida albicans (C. albicans) infection without raising the concern of drug resistance. Despite its effectiveness, a higher dose phototherapeutic power required for C. elimination compared bacteria that have be used, which readily accompanied by off-target heat and toxic singlet oxygen damage normal cells, thus limiting usefulness antifungal applications. Here overcome this, we develop "three-in-one" biomimetic nanoplatform consisting...
Background M. morganii is a species of the genus Morganella in family Enterobacteriaceae . This primarily causes infections postoperative wounds and urinary tract. Some isolates exhibit resistance to multiple antibiotics due multidrug traits, complicating clinical treatment; thus, there growing need elucidate mechanisms this pathogen. Methods A total 658 bacterial strains were isolated from anal fecal swabs poultry livestock surrounding environment Wenzhou, China, via plate streaking. The...
The emergence of highly drug-resistant K. pneumoniae, has become a major public health challenge. In this work, we aim to investigate the diversity species and sequence types (STs) clinical Klebsiella isolates characterize prevalence structure class 1 integrons.Based on whole genome sequencing, identification was performed by 16S rRNA gene homology average nucleotide identity (ANI) analysis. STs were determined in accordance with international MLST schemes for pneumoniae variicola. Integron...
To exam the relationship between snoring and morbidities of multiple systems in children.Children with were enrolled divided into primary snorer (PS) group obstructive sleep apnea hypopnea syndrome (OSAHS) based on polysomnography. The healthy children served as control group. growth parameters, maxillofacial malformations, blood chemistry, electrocardiogram, echocardiogram recorded intelligence testing was performed who ≥6 years old.The weight height similar (n = 60) PS 63), but lower OSAHS...
Background The mechanisms underlying the resistance of genus Providencia to aminoglycosides are complex, which poses a challenge for efficient treatment infectious diseases caused by these pathogens. To help clinicians treat infections more effectively, comprehensive understanding antibiotic is urgently needed. Methods Plates were streaked isolate bacteria from intestinal contents fish. standard agar dilution method was used determine minimum inhibitory concentrations (MICs) antimicrobial...
In this work, by high-throughput sequencing, antibiotic resistance genes, including class A ( bla CTX-M , Z TEM VEB KLUC and SFO ), C SHV DHA MIR AZECL-29 ACT D OXA ) β -lactamase were identified among the pooled genomic DNA from 212 clinical Enterobacter cloacae isolates. Six -positive E. strains identified, pulsed-field gel electrophoresis (PFGE) showed that these not clonally related. The complete genome of - positive strain (Y546) consisted both a chromosome (4.78 Mb) large plasmid pY546...
This study was designed to characterize the dissemination mechanism and genetic context of Klebsiella pneumoniae carbapenemase (KPC) genes in carbapenem-resistant (CRKP) isolates.A retrospective analysis performed on CRKP strains isolated from a teaching hospital Wenzhou Medical University during 2015-2017. Polymerase chain reaction (PCR)-based amplification whole-genome sequencing (WGS) were used analyze blaKPC-2 gene. Conjugation experiments evaluate transferability blaKPC-2-bearing...
Hepatocellular carcinoma (HCC) is one of the most common malignant tumors in clinic, with characteristics occult onset, rapid progression, and high degree malignancy. Alpha fetoprotein (AFP) important biomarker HCC, which widely used early screening, diagnosis, prognosis observation. A series immunoassays have been developed frequently detection AFP based on antibodies. Unfortunately, shortcomings antibodies, such as thermal unstable fluctuant activity by batches, lead to inaccuracy AFP. In...
Members of the Enterobacter cloacae complex are important opportunistic human pathogens capable causing a wide variety infections. During recent decades, aminoglycoside-resistant E. isolates have increasingly been reported and become major concern. Here, we employed high-throughput sequencing in combination with specific PCR assays to investigate prevalence aminoglycoside resistance genes among 170 collected from teaching hospital Wenzhou, China. A total 12 known [aphA-1, strA, strB,...
Background Aeromonas species have been identified as agents responsible for various diseases in both humans and animals. Multidrug-resistant strains pose a significant public health threat due to their emergence spread clinical settings the environment. The aim of this study was determine novel resistance mechanism against aminoglycoside antimicrobials isolate. Methods function aac(6′)-Va verified by gene cloning antibiotic susceptibility tests. To explore vivo activity enzyme, recombinant...
A novel chromosome-encoded aminoglycoside O -nucleotidyltransferase AadA33 was identified in Providencia vermicola strain P13. The shares the highest amino acid identity of 51.28% with function characterized AadA31. Antibiotic susceptibility testing and enzyme kinetics analysis revealed that is to mediate spectinomycin streptomycin resistance. recombinant harboring aadA33 (pUCP20- / Escherichia coli DH5α) displayed >256- 128-fold increases minimum inhibitory concentration levels...
Extracting facade maps from 3D point clouds is a fast and economical way to describe building’s surface structure. Existing methods lack efficiency, robustness, accuracy, depend on many additional features such as cloud reflectivity color. This paper proposes robust automatic method extract building maps. First, an improved Hough transform proposed by adding shift vote convolution of the accumulator improve computational efficiency reduce peak fuzziness dependence step selection. These...
To investigate molecular mechanism of multi-resistance Klebsiella pneumoniae and its spreading, 179 strains isolated from different clinical samples in the period 2002-2007 with serious resistance to 14 anti-bacterial agents were examined. Among them, 118 (65.9%) resistant at least two agents; 36.3% (65/179) found contain class 1 integrons. There was a significant difference for rate between integron positive negative groups, especially antimicrobial aminoglycosides, quinolones sulfonamides...
In order to study the relationship between structure and function of AmpG, structure, site-specific mutation, gene complementary experiments have been performed against clinical isolates Pseudomonas aeruginosa . We found that there are 51 nucleotide variations at 34 loci over ampG genes from 24 35 P. strains detected, which 7 resulted in amino acid change. The variants with changed nucleotides (amino acids) could complement deleted PA01 (PA01ΔG). ampicillin minimum inhibitory concentration...
To analyze clinical manifestation and genetic mutations in 8 Chinese pedigrees featuring hereditary dysfibrinogenemia.Prothrombin time(PT), activated partial thromboplastin time(APTT), thrombin time(TT), calibration of plasma protamine sulfate against TT, fibrinogen (Fg) activity, coagulation factors II, V, VII, VIII, IX, X, XI XII all probands their family members were detected with an automatic blood analyzer; D-dimer(D-D) fibrin(ogen) degradation products(FDPs) also dtected by analyzer,...
Background The intrinsic resistance mechanism plays an essential role in the bacterial to a variety of antimicrobials. aim this study is find chromosome-encoded novel antimicrobial gene clinical isolate. Methods function predicted was verified by cloning and antibiotic susceptibility test. Recombinant protein expression enzyme kinetic studies were performed explore vivo activity enzyme. Expression exposed determined RT-qPCR. Whole genome sequencing bioinformatic analysis applied analyze...
A novel plasmid-encoded aminoglycoside 3''-nucleotidyltransferase ANT(3")-IId, was discovered in Acinetobacter lwoffi strain H7 isolated from a chick on an animal farm Wenzhou, China. The whole-genome of A. lwoffii consisted one chromosome and five plasmids (pH7-250, pH7-108, pH7-68, pH7-48, pH7-11). ant(3")-IId identified as being encoded pH7-250, sharing the highest amino acid identity 50.64% with function-known resistance gene, ant(3")-IIb (KB849358.1). Susceptibility testing enzyme...
In order to deal with the defect of slow convergence rate and singularity classical sliding mode control finite time in flexible robotic arm problems, a method based on dynamic surface is designed realize tracking reference signals. Firstly, improved by introducing means update parameters real achieve system surface, magnified are updated parametric number error angle fractional achieved. Then finite-time controller fractional-order state feedback said for trajectory. Finally, validity...