A5089648483- Autophagy in Disease and Therapy
- Cellular transport and secretion
- MicroRNA in disease regulation
- Neurogenesis and neuroplasticity mechanisms
- Endoplasmic Reticulum Stress and Disease
- Calcium signaling and nucleotide metabolism
- Glioma Diagnosis and Treatment
- Single-cell and spatial transcriptomics
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Hippo pathway signaling and YAP/TAZ
- Genetics, Aging, and Longevity in Model Organisms
- Epigenetics and DNA Methylation
- Histone Deacetylase Inhibitors Research
- Ubiquitin and proteasome pathways
- Genetics and Neurodevelopmental Disorders
- Immune cells in cancer
- Cloud Computing and Resource Management
- CAR-T cell therapy research
- Ion Transport and Channel Regulation
- ATP Synthase and ATPases Research
- Neurofibromatosis and Schwannoma Cases
- Lysosomal Storage Disorders Research
- Chromatin Remodeling and Cancer
- Genomics and Chromatin Dynamics
Cincinnati Children's Hospital Medical Center
2018-2024
Fudan University
2021
Institute of Genetics and Developmental Biology
2013-2018
Chinese Academy of Sciences
2013-2018
Yunnan University
2017
Center for Life Sciences
2017
University of Chinese Academy of Sciences
2016
Intel (United States)
2010
Phosphatidylinositol 3-phosphate (PtdIns3P) plays a central role in endosome fusion, recycling, sorting, and early-to-late conversion, but the mechanisms that determine how correct endosomal PtdIns3P level is achieved remain largely elusive. Here we identify two new factors, SORF-1 SORF-2, as essential regulators Caenorhabditis elegans. Loss of sorf-1 or sorf-2 leads to greatly elevated PtdIns3P, which drives excessive fusion early endosomes. function coordinately with Rab switching genes...
Autophagy-dependent clearance of ubiquitinated and aggregated proteins is critical to protein quality control, but the underlying mechanisms are not well understood. Here, we report essential role BEACH (beige Chediak-Higashi) WD40 repeat-containing WDR81 in eliminating through autophagy. associates with ubiquitin (Ub)-positive foci, its loss causes accumulation Ub autophagy cargo receptor p62. interacts p62, facilitating recognition by Furthermore, LC3C canonical LC3-interacting regions...
Clathrin and the multi-subunit adaptor protein complex AP2 are central players in clathrin-mediated endocytosis by which cell selectively internalizes surface materials. Here, we report essential role of clathrin phagocytosis apoptotic cells. In Caenorhabditis elegans, depletion heavy chain CHC-1 individual components led to a significant accumulation germ corpses, resulted from defects both corpse engulfment phagosome maturation required for removal. associate with phagosomes an...
Malignant peripheral nerve sheath tumor (MPNST), a highly aggressive Schwann cell (SC)–derived soft tissue sarcoma, arises from benign neurofibroma (NF); however, the identity, heterogeneity and origins of populations remain elusive. Nestin + cells have been implicated as stem in MPNST; unexpectedly, single-cell profiling human NF MPNST their animal models reveal broad range nestin-expressing SC lineage dynamic acquisition discrete cancer states during malignant transformation. We uncover...
The oligodendrocyte (OL) lineage transcription factor Olig2 is expressed throughout oligodendroglial development and essential for progenitor specification differentiation. It was previously reported that deletion of enhanced the maturation myelination immature OLs accelerated remyelination process. However, by analyzing multiple conditional KO mouse lines (male female), we conclude has opposite effect required OL remyelination. We found in driven an OL-expressing Plp1 promoter resulted...
Abstract Adult hippocampal neurogenesis, a process considered important for function, is regulated at multiple molecular levels. Mutations in the gene encoding WD40 repeat-containing protein WDR81 are associated with neurological disorders, including cerebellar ataxia, mental retardation, quadrupedal locomotion syndrome (CAMRQ2), and microcephaly. In this study, we show that ablation of adult neural progenitor cells (aNPCs) markedly reduced neurogenesis impaired hippocampus-dependent...
Abstract Lysosomes are degradation and signaling centers within the cell, their dysfunction impairs a wide variety of cellular processes. To understand effect lysosome damage, we screened natural small-molecule compounds that induce lysosomal abnormality using Caenorhabditis elegans (C. elegans) as model system. A group vobasinyl-ibogan type bisindole alkaloids (ervachinines A–D) were identified caused enlargement in C. macrophage-like cells. Intriguingly, these triggered cell death germ...
Chromatin-remodeling protein BRG1/SMARCA4 is pivotal for establishing oligodendrocyte (OL) lineage identity. However, its functions oligodendrocyte-precursor cell (OPC) differentiation within the postnatal brain and during remyelination remain elusive. Here, we demonstrate that Brg1 loss profoundly impairs OPC in with a comparatively lesser effect spinal cord. Moreover, BRG1 critical after injury. Integrative transcriptomic/genomic profiling reveals exhibits dual role by promoting networks...
More and more novel usage models require the capability of resource sharing among different platforms. To achieve a satisfactory efficiency, we introduce specific technology under which IO peripherals can be shared In particular, in personal working environment that is built up by number devices, at each device applied to support application running another device. This our so-called composable because it equivalent compose IOs from devices for an application. We design logic pro-migration...
Neurodegenerative diseases are characterized by neuron loss and accumulation of undegraded protein aggregates. These phenotypes partially due to defective degradation in neuronal cells. Autophagic clearance aggregated proteins is critical quality control, but the underlying mechanisms still poorly understood. Here we report essential role WDR81 autophagic aggregates models Huntington’s disease (HD), Parkinson’s (PD) Alzheimer’s (AD). In hippocampus cortex patients with HD, PD AD, level...
Abstract Progenitor heterogeneity and identities underlying tumor initiation relapse in medulloblastomas, the most common malignant pediatric brain tumor, remain elusive. Here, by utilizing single-cell analysis at different stages of tumorigenesis, we demonstrated a developmental hierarchy diverse progenitor pools sonic hedgehog (SHH)-medulloblastomas. Unexpectedly, identified Olig2-expressing progenitors as transit-amplifying cells onset tumorigenesis. Although Olig2+ become quiescent...
PLOS Genetics Editors that results from experiments reported in Fig 5M and5N were not reproducible follow-up experiments, raising questions about the validity of main conclusions.In and 5N this article [1], authors report using fibroblasts two Huntington's disease (HD) patients with different polyQ expansions (Htt47Q, n = 1; Htt68Q, control individuals, 2).These are derived three independent experiments.However, after publication collected more samples HD individuals for further...
Abstract Medulloblastoma (MB) is the most common malignant childhood brain tumor 1,2 , yet origin of aggressive subgroup-3 form remains elusive, impeding development effective targeted treatments. Previous analyses mouse cerebella 3,4 or human counterparts from frozen tissue nuclei 5 have not fully defined compositional heterogeneity MBs. Here, we undertook an unprecedented single-cell profiling freshly-isolated fetal to establish a reference map delineating hierarchical cellular states in...