A5055427714- Genetics, Aging, and Longevity in Model Organisms
- Reproductive Biology and Fertility
- Selenium in Biological Systems
- Sperm and Testicular Function
- Mitochondrial Function and Pathology
- Cellular Mechanics and Interactions
- Photosynthetic Processes and Mechanisms
- CRISPR and Genetic Engineering
- Trace Elements in Health
- Soybean genetics and cultivation
- Autophagy in Disease and Therapy
- Micro and Nano Robotics
- Microtubule and mitosis dynamics
- Biocrusts and Microbial Ecology
- Parasite Biology and Host Interactions
- Phagocytosis and Immune Regulation
- Plant Disease Resistance and Genetics
- Plant nutrient uptake and metabolism
- Circadian rhythm and melatonin
- Plant Stress Responses and Tolerance
- Insect Resistance and Genetics
- Cellular transport and secretion
- Legume Nitrogen Fixing Symbiosis
- Cancer-related gene regulation
- Nanoparticles: synthesis and applications
Beijing Normal University
2022-2025
Institute of Biophysics
2009-2024
Chinese Academy of Sciences
2011-2024
University of Chinese Academy of Sciences
2018-2024
University of Tennessee at Knoxville
2020
Southeast University
2016
Anhui Medical University
2015
China Agricultural University
2004-2010
Florida State University
2003-2008
MRC Laboratory of Molecular Biology
2003
The cell surface receptor CED-1 mediates apoptotic recognition by phagocytic cells, enabling corpse clearance in Caenorhabditis elegans. Here, we found that the C. elegans intracellular protein sorting complex, retromer, was required for mediating recycling of CED-1. Retromer recruited to surfaces phagosomes containing corpses, and its loss function caused defective removal. retromer probably acted through direct interaction with pathway. In absence function, associated lysosomes failed...
Abstract Plant viral movement proteins (MPs) enable viruses to pass through cell walls by increasing the size exclusion limit (SEL) of plasmodesmata (PD). Here, we report that ability Cucumber mosaic virus (CMV) MP increase SEL PD could be inhibited treatment with actin filament (F-actin)–stabilizing agent phalloidin but not F-actin–destabilizing latrunculin A. In vitro studies showed CMV bound globular and F-actin, polymerization, severed participated in plus end capping F-actin. Analyses...
Lysosomes degrade macromolecules and recycle metabolites as well being involved in diverse processes that regulate cellular homeostasis. The lysosome is limited by a single phospholipid bilayer forms barrier to separate the potent luminal hydrolases from other constituents, thus protecting latter unwanted degradation. mechanisms maintain lysosomal membrane integrity remain unknown. Here, we identified SCAV-3, Caenorhabditis elegans homologue of human LIMP-2, key regulator integrity,...
Although sperm is transcriptionally and translationally quiescent, complex populations of RNAs, including mRNAs non-coding exist in sperm. Previous microarray analysis germ cell mutants identified hundreds genes Caenorhabditis elegans. To take a more comprehensive view on C. elegans genes, here, we isolate highly pure cells employ high-throughput technologies to obtain transcriptome proteome. First, consists considerable amounts many which have not been annotated may play functional roles...
Abstract In most eukaryotes, mitochondria are inherited maternally. The autophagy process is critical for paternal mitochondrial elimination (PME) in Caenorhabditis elegans , but how mitochondria, not maternal selectively targeted degradation poorly understood. Here we report that dynamics have a profound effect on PME. A defect fission of delays PME, whereas fusion accelerates Surprisingly, which reversed by or increasing membrane potential using oligomycin. Electron microscopy and...
Soybean nodulation is a highly controlled process that involves complex gene regulation at both transcriptional and post-transcriptional levels. In the present study, we profiled expression changes, alternative splicing events, DNA methylation patterns during nodule formation, development, senescence. The transcriptome data uncovered key transcription of development included 9669 core genes 7302 stage-specific genes. Alternative analysis total 2323 undergo events in least one developmental...
Significance Zinc plays important roles in numerous cellular processes. Deficiency or excess of Zn 2+ leads to many diseases. concentration at various compartments is regulated. Imbalance mitochondria has been linked neurodegeneration. However, little known about how mitochondrial We find that SLC-30A9 required for export from both Caenorhabditis elegans and human cells. Loss slc-30a9 excessive accumulation mitochondria, severe swelling, compromised metabolic function, reductive stress,...
Spermiogenesis is a series of poorly understood morphological, physiological and biochemical processes that occur during the transition immotile spermatids into motile, fertilization-competent spermatozoa. Here, we identified Serpin (serine protease inhibitor) family protein (As_SRP-1) secreted from nematode Ascaris suum spermiogenesis (also called sperm activation) showed As_SRP-1 has two major functions. First, functions in cis to support (MSP)-based cytoskeletal assembly spermatid...
Immotile spermatids produced in the testis must undergo a series of poorly understood morphological, physiological and biochemical processes called sperm activation to become motile, fertilization-competent spermatozoa. In Caenorhabditis elegans, spe-8 group contains sperm-specific genes active both males hermaphrodites, although their activity is required only for hermaphrodite self-sperm activation. The activating signal upstream SPE-8 signaling cascade remains unknown. Here, we show that...
Sperm activation is a fascinating example of cell differentiation, in which immotile spermatids undergo rapid and dramatic transition to become mature, motile sperm. Because the sperm nucleus transcriptionally silent, this does not involve transcriptional changes. Although Caenorhabditis elegans leading model for studies activation, mechanisms by signaling pathways induce transformation remain poorly characterized. Here we show that conserved transmembrane zinc transporter, ZIPT-7.1,...
DNA methylation is a widespread epigenetic mark that affects gene expression and transposon mobility during plant development stress responses. However, the role of in regulating microRNA (miRNA) genes remains largely unexplored. Here, we analyzed changes miRNA using pair soybean (Glycine max) near-isogenic lines (NILs) differing their response to cyst nematode (SCN; Heterodera glycines). Differences global levels over SCN infection were observed between isogenic lines. with significant...
Cells crawl by coupling protrusion of their leading edge with retraction cell body. Protrusion is generated the polymerization and bundling filaments, but mechanism less clear. We have reconstituted in vitro adding Yersinia tyrosine phosphatase to major sperm protein–based motility apparatus assembled from Ascaris extracts. Retraction parallels that observed vivo primarily disassembly rearrangement cytoskeleton. Therefore, cytoskeletal dynamics alone, unassisted conventional motors, are able...
Clathrin and the multi-subunit adaptor protein complex AP2 are central players in clathrin-mediated endocytosis by which cell selectively internalizes surface materials. Here, we report essential role of clathrin phagocytosis apoptotic cells. In Caenorhabditis elegans, depletion heavy chain CHC-1 individual components led to a significant accumulation germ corpses, resulted from defects both corpse engulfment phagosome maturation required for removal. associate with phagosomes an...
Hybrid incompatibility (HI) prevents gene flow between species, thus lying at the heart of speciation genetics. One most common HIs is male sterility. Two superficially contradictory observations exist for hybrid First, an introgression on X Chromosome more likely to produce sterility than autosome (so-called large-X theory); second, spermatogenesis genes are enriched autosomes but depleted (demasculinization Chromosome). Analysis expression in Drosophila hybrids suggests a genetic...
Mitochondria are the powerhouses of many biological processes. During spermatogenesis, post-transcriptional regulation mitochondrial gene expression is mediated by nuclear-encoded RNA-binding proteins (mtRBPs). We identified AMG-1 as an mtRBP required for reproductive success in C. elegans. amg-1 mutation led to defects structure and sperm budding, resulting mitochondria being discarded into residual bodies (RBs), which ultimately delayed spermatogenesis proximal gonad. In addition,...
Although several models have been proposed to account for how cytoskeleton polymerization drives protrusion in cell motility, the precise mechanism remains controversial. Here, we show that, addition force exerted directly against membrane by growing filaments, way elongating filaments pack also contributes generating an expansion of gel. Tomography shows that filament packing major sperm protein (MSP) -based nematode sperm-motility machinery resembles observed with rigid rods. Maximum...