A5057712848- Photosynthetic Processes and Mechanisms
- Microbial Community Ecology and Physiology
- Algal biology and biofuel production
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Glycosylation and Glycoproteins Research
- Metabolomics and Mass Spectrometry Studies
- Biosensors and Analytical Detection
- ATP Synthase and ATPases Research
- Ecology and Conservation Studies
- Genomics and Phylogenetic Studies
- Protein Structure and Dynamics
- Heat shock proteins research
- Plant-Microbe Interactions and Immunity
- Nuclear Physics and Applications
- Proteoglycans and glycosaminoglycans research
- Cell Adhesion Molecules Research
- Electrochemical sensors and biosensors
- Bioactive natural compounds
- Plant Gene Expression Analysis
- Genetics, Aging, and Longevity in Model Organisms
- Ion Transport and Channel Regulation
- Thyroid Cancer Diagnosis and Treatment
- Phagocytosis and Immune Regulation
- Cellular transport and secretion
Institute of Genetics and Developmental Biology
2015-2024
Chinese Academy of Sciences
2015-2024
Kunming University of Science and Technology
2021
University of Chinese Academy of Sciences
2016
Chinese Academy of Forestry
2014
Zhejiang University
2013
Emerging evidences exhibit that mitogen-activated protein kinase (MAPK/MPK) signaling pathways are connected with many aspects of plant development. The complexity MAPK cascades raises challenges not only to identify the module in planta but also define specific role an individual module. So far, our knowledge has been largely restricted a small subset cascades. Our previous study characterized Arabidopsis bushy and dwarf1 (bud1) mutant, which MAP Kinase 7 (MKK7) was constitutively...
Clathrin and the multi-subunit adaptor protein complex AP2 are central players in clathrin-mediated endocytosis by which cell selectively internalizes surface materials. Here, we report essential role of clathrin phagocytosis apoptotic cells. In Caenorhabditis elegans, depletion heavy chain CHC-1 individual components led to a significant accumulation germ corpses, resulted from defects both corpse engulfment phagosome maturation required for removal. associate with phagosomes an...
The histidine kinase Hik33 plays important roles in mediating cyanobacterial response to divergent types of abiotic stresses including cold, salt, high light (HL), and osmotic stresses. However, how these functions are regulated by remains be addressed. Using a hik33-deficient strain (Δhik33) Synechocystis sp. PCC 6803 (Synechocystis) quantitative proteomics, we found that depletion induces differential protein expression highly like induced This typically includes downregulation proteins...
Cyanobacteria are a group of oxygenic photosynthetic bacteria with great potentials in biotechnological applications and advantages as models for photosynthesis research. The subcellular localizations the majority proteins any cyanobacteria remain undetermined, representing major challenge using both basic industrial researches. Here, label-free quantitative proteomics, we map 2027 Synechocystis sp. PCC6803, model cyanobacterium, to different compartments generate proteome atlas such...
Carbon metabolism is central to photosynthetic organisms and involves the coordinated operation regulation of numerous proteins. In cyanobacteria, proteins involved in carbon are regulated by multiple regulators including RNA polymerase sigma factor SigE, histidine kinases Hik8, Hik31 its plasmid-borne paralog Slr6041, response regulator Rre37. To understand specificity cross-talk such regulations, we simultaneously quantitatively compared proteomes gene knockout mutants for regulators. A...
Large-scale quantitative evaluation of the tightness membrane association for nontransmembrane proteins is important identifying true peripheral with functional significance. Herein, we simultaneously ranked more than 1000 photosynthetic model organism Synechocystis sp. PCC 6803 their relative using a proteomic approach. Using multiple precisely and experimentally verified subunits protein complexes as landmarks, found that involved in two-component signal transduction systems transporters...
Abstract The mitogen-activated protein kinase ERK1/2 (ERKs, extracellular-regulated kinases) plays important roles in a wide spectrum of cellular processes and have been implicated many disease states. spatiotemporal regulation ERK activity has extensively studied. However, scarce information available regarding the quality control kinases to scavenge malfunctioning ERKs. Using site-specific mutagenesis mass spectrometry, we found that disruption conserved H-bond between Y210 E237 ERK1...
Abstract Protein tyrosine phosphorylation is an important mechanism that regulates cytoskeleton reorganization and cell spreading of migratory cells. A number cytoskeletal proteins are known to be phosphorylated (pY) in different cellular processes. However, the profile pY during stages has not been available. Using immunoafffinity enrichment coupled with label free quantitative proteomics, we quantitatively identified 447 ECV-304 cells at early (adhesion) active stages. We found levels...
The exoproteome is one of the important components extracellular milieu unicellular cyanobacterium Synechocystis sp.PCC 6803, a photosynthetic model organism that emerging as potential cell factory for producing clean and renewable biofuel.Herein, we identified this using high resolution mass spectrometry analyzed sources origins proteins.In total, 201 proteins were identified, including surface protein HlyA periplasmic FutA2 are most abundant in exoproteome.The combined amount two estimated...
Spatial proteome reorganization in response to a changing environment represents different layer of adaptation mechanism addition differential expression subset stress responsive genes photosynthetic organisms. Profiling such events is critically important extend our understanding how organisms adapt adverse environments. Thus, we treated unicellular model cyanobacterium, Synechocystis sp. PCC 6803 (hereafter referred as Synechocystis), with five types abiotic stresses including nitrogen...
Ascorbate peroxidase (APEX)-based proximity labeling coupled with mass spectrometry has a great potential for spatiotemporal identification of proteins proximal to protein complex interest. Using this approach is feasible define the proteome neighborhood important complexes in popular photosynthetic model cyanobacterium Synechocystis sp. PCC6803 (hereafter named as Synechocystis). To end, we developed robust workflow APEX2-based and used identify photosystem II (PS II) oxygen evolution (OEC)...
Summary Glycosyltransferases (GTs) are enzymes that transfer sugars to various targets. They play important roles in diverse biological processes, including photosynthesis, cell motility, exopolysaccharide biosynthesis, and lipid metabolism; however, their involvement regulating carbon metabolism Synechocystis sp. PCC 6803 has not been reported. We identified a novel GT protein, Slr1064, involved metabolism. The effect of slr1064 deletion on the growth cells functional mechanisms Slr1064...
The histidine kinase Hik33 plays a central role in acclimation to changing environments cyanobacteria. Deletion of hik33 induces strong stress-like response Synechocystis sp. PCC 6803 (Synechocystis) as represented by repressed photoautotrophic growth and photosynthesis, differential expression stress-responsive proteins. In contrast, the photomixotrophic hik33-deletion mutant (Δhik33) with glucose exogenous carbon source is only marginally repressed. To investigate how rescues Δhik33,...
The recent development and implementation of the advanced peak determination (APD) algorithm with MS instrument dramatically increased sampling low abundance features for MS/MS fragmentation. After in-depth evaluation, it is found that APD on, many chimeric spectra are acquired through co-fragmentation high contaminants targets, such co-fragmentations largely avoided when off. To evaluate whether a could significantly distort accuracy isobaric-labeling based quantitation target, single-shot...
Liquid chromatography-mass spectrometry (LC-MS) is a major tool for the large-scale qualitative and/or quantitative analysis of protein phosphorylation in cells or tissues. The performance LC pivotal success phosphoproteomics both sensitivity and reproducibility. Here, we report that widely used Easy-nLC 1200 has poor analyzing phosphopeptides, particularly terms reproducibility, whereas its predecessor, 1000, much better performance. Therefore, suggest not appropriate LC-MS-based proteomics...