In plants, cadmium (Cd)-responsive transcription factors are key downstream effectors of Cd stress transcriptional pathways, which capable converging signals through triggering the expression detoxification genes. However, upstream regulatory pathways that modulate their responses to less clear. Previously, we identified bean (Phaseolus vulgaris) METAL RESPONSE ELEMENT-BINDING TRANSCRIPTION FACTOR1 (PvMTF-1) responds and confers tolerance in planta. Here, demonstrate an regulation PvMTF-1...

Asthenozoospermia (ASZ) accounts for about 20-40% of male infertility, and genetic factors, contributing to 30-40% the causes ASZ, still need further exploration. Radial spokes (RSs), a T-shaped macromolecular complex, connect peripheral doublet microtubules (DMTs) central pair (CP), forming CP-RS-DMT structure regulate beat frequency amplitude sperm flagella. To date, many components RSs their functions in human flagella remain unclear. We recruited cohort 323 infertile males with ASZ...

Background: Intermittent fasting (IF) has been increasingly recognized for its potential to mitigate obesity and diabetes. However, it remains unclear whether IF can alleviate metabolic disorder-induced male infertility. The aim of this study was investigate the improve fertility outcomes in obese mice. Methods: Eight-week-old C57BL/6J mice were fed a high-fat diet (HFD) 24 weeks induce obesity, followed by alternate-day 6 weeks. We assessed obesity-related changes issues postintervention....

Multiple morphological abnormalities of sperm flagella is an idiopathic asthenoteratozoospermia characterized by absent, short, coiled, angulation, and irregular-caliber flagella. Genetic variants DNAH1 gene have been identified as a causative factor multiple intracytoplasmic injection available strategy for infertile males with dynein axonemal heavy chain 1 defects to conceive.

Abstract STUDY QUESTION Could actin-related protein T1 (ACTRT1) deficiency be a potential pathogenic factor of human male infertility? SUMMARY ANSWER A 110-kb microdeletion the X chromosome, only including ACTRT1 gene, was identified as responsible for infertility in two Chinese males with sperm showing acrosomal ultrastructural defects and fertilization failure. WHAT IS KNOWN ALREADY The proteins (e.g. ACTRT1, ACTRT2, ACTL7A, ACTL9) interact each other to form multimeric complex...

Hybrid incompatibility (HI) prevents gene flow between species, thus lying at the heart of speciation genetics. One most common HIs is male sterility. Two superficially contradictory observations exist for hybrid First, an introgression on X Chromosome more likely to produce sterility than autosome (so-called large-X theory); second, spermatogenesis genes are enriched autosomes but depleted (demasculinization Chromosome). Analysis expression in Drosophila hybrids suggests a genetic...

Abstract Background Dysfunction of motile cilia, including respiratory cilia and sperm flagella, typically leads to primary ciliary dyskinesia male infertility or low fertility in humans. Genetic defects LRRC6 have been associated with asthenozoospermia due abnormal ultrastructure ciliated axonemes. Objectives To identify novel mutations the gene related multiple morphological abnormalities flagella investigate underlying molecular mechanisms involved. Materials methods The were identified...

As a specific type of asthenoteratozoospermia, multiple morphological abnormalities the sperm flagella (MMAF) is characterized by composite abnormalities, including absent, short, coiled, angulation, and irregular-caliber flagella. Mutations in cilia- flagella-associated protein 43 ( CFAP43 ) are one main causative factors MMAF established to date. To identify whether there other mutations related determine clinical outcomes assisted reproductive technology for patients with harboring...

Abstract Study question Is there any novel variants and mutant hotspot in DNAH1 gene related to multiple morphological abnormalities of sperm flagella (MMAF) male infertility humans? Summary answer We identified eighteen eleven MMAF-affected Chinese men, including fourteen variants, four previously reported a candidate hotspot. What is known already As specific type asthenoteratozoospermia, MMAF characterized by composite flagellar defects absent, short, coiled, angulation,...

Axonemal protein complexes, including the outer and inner dynein arms (ODA/IDA), are highly ordered structures of sperm flagella that drive motility. Deficiencies in several axonemal proteins have been associated with male infertility, which is characterized by asthenozoospermia or asthenoteratozoospermia. Dynein heavy chain 3 (DNAH3) resides IDA expressed testis. However, relationship between DNAH3 infertility still unclear. Herein, we identified biallelic variants four unrelated Han...

Axonemal protein complexes, including the outer and inner dynein arms (ODA/IDA), are highly ordered structures of sperm flagella that drive motility. Deficiencies in several axonemal proteins have been associated with male infertility, which is characterized by asthenozoospermia or asthenoteratozoospermia. Dynein heavy chain 3 (DNAH3) resides IDA expressed testis. However, relationship between DNAH3 infertility still unclear. Herein, we identified biallelic variants four unrelated Han...

Ethylene-responsive elements (EREs), such as the GCC box, are critical for ethylene-regulated transcription in plants. Our previous work identified a 19-bp AC-rich element (ACE) promoter of bean (Phaseolus vulgaris) metal response element-binding factor 1 (PvMTF-1). Ethylene 15 (PvERF15) directly binds ACE to enhance PvMTF-1 expression. As novel ERF-binding element, exhibits significant difference from box. Here, we demonstrated that serves an ERE Arabidopsis. It conferred minimal respond...

The genotype-phenotype relationships between TUBB8 variants and female infertility are difficult to clearly define due the complex inheritance patterns highly heterogeneous phenotypes. This study aims identify novel relevant phenotypes in more infertile females. A total of 35 females with primary were recruited from two reproductive centers investigated for identifying TUBB8. Pedigree analysis, in-silico analysis molecular remodeling performed assess their clinical significance. effects on...

Purpose To study whether physical exercise can effectively ameliorate obesity-induced abnormalities in male fertility and provide a new perspective on the role of small noncoding RNAs spermatogenesis obese mice. Methods In this study, four-week-old C57/Bl6 mice were randomly allocated to receive control diet, high-fat diet or intervention for 40 weeks. Purified round spermatids spermatozoa obtained after intervention. Sperm motility, concentration, ability sperm undergo capacitation acrosome...

Meckel syndrome (MKS), also known as the Meckel-Gruber syndrome, is a severe pleiotropic autosomal recessive developmental disorder caused by dysfunction of primary cilia during early embryogenesis. The diagnostic criteria are based on clinical variability and genetic heterogeneity. Mutations in MKS1 gene constitute approximately 7% all MKS cases. Herein, we present non-consanguineous couple with three abnormal pregnancies fetuses showed MKS-related phenotypes central nervous system...

Axonemal protein complexes, including the outer and inner dynein arms (ODA/IDA), are highly ordered structures of sperm flagella that drive motility. Deficiencies in several axonemal proteins have been associated with male infertility, which is characterized by asthenozoospermia or asthenoteratozoospermia. Dynein heavy chain 3 (DNAH3) resides IDA expressed testis. However, relationship between DNAH3 infertility still unclear. Herein, we identified biallelic variants four unrelated Han...

Axonemal protein complexes, including the outer and inner dynein arms (ODA/IDA), are highly ordered structures of sperm flagella that drive motility. Deficiencies in several axonemal proteins have been associated with male infertility, which is characterized by asthenozoospermia or asthenoteratozoospermia. Dynein heavy chain 3 (DNAH3) resides IDA expressed testis. However, relationship between DNAH3 infertility still unclear. Herein, we identified biallelic variants four unrelated Han...

Axonemal protein complexes, including the outer and inner dynein arms (ODA/IDA), are highly ordered structures of sperm flagella that drive motility. Deficiencies in several axonemal proteins have been associated with male infertility, which is characterized by asthenozoospermia or asthenoteratozoospermia. Dynein heavy chain 3 (DNAH3) resides IDA expressed testis. However, relationship between DNAH3 infertility still unclear. Herein, we identified biallelic variants four unrelated Han...