A5001207838- Reproductive Biology and Fertility
- Assisted Reproductive Technology and Twin Pregnancy
- Prenatal Screening and Diagnostics
- Sperm and Testicular Function
- Reproductive Health and Technologies
- Renal and related cancers
- Ovarian function and disorders
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Molecular Biology Techniques and Applications
- Sexual Differentiation and Disorders
- Genetic and Kidney Cyst Diseases
- Neuroscience and Neural Engineering
- Ultrasound and Hyperthermia Applications
- Selenium in Biological Systems
- Digital Transformation in Law
- Cancer Genomics and Diagnostics
- Artificial Intelligence in Healthcare and Education
- Nuclear Structure and Function
- Reproductive System and Pregnancy
- Urological Disorders and Treatments
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
Chongqing Maternal and Child Health Hospital
2013-2025
Children's Hospital of Chongqing Medical University
1998-2025
Chongqing Medical University
1998-2025
Chongqing Institute of Genetics and Reproduction
2011-2016
Lactate is present at a high level in the microenvironment of mammalian preimplantation embryos vivo and vitro. However, its role development unclear. Here, we report that lactate highly enriched nuclei early when major zygotic genome activation (ZGA) occurs humans mice. The inhibition production uptake results developmental arrest 2-cell stage, ZGA failure, loss lactate-derived H3K18lac, which could be rescued by addition Lac-CoA recapitulated overexpression H3K18R mutation. By profiling...
Asthenozoospermia (ASZ) accounts for about 20-40% of male infertility, and genetic factors, contributing to 30-40% the causes ASZ, still need further exploration. Radial spokes (RSs), a T-shaped macromolecular complex, connect peripheral doublet microtubules (DMTs) central pair (CP), forming CP-RS-DMT structure regulate beat frequency amplitude sperm flagella. To date, many components RSs their functions in human flagella remain unclear. We recruited cohort 323 infertile males with ASZ...
Abstract Background Many studies have demonstrated the benefits of addition growth hormone (GH) to controlled ovarian stimulation protocol in vitro fertilization (IVF) cycles poor-respond patients, but effect GH on patients with poor embryonic development remain unclear. This paper was designed investigate efficacy co-treatment during IVF for development. Method A randomized trial including 158 embryo conducted between July 2017 and February 2019. One hundred seven were treatment (GH group)...
Multiple morphological abnormalities of sperm flagella is an idiopathic asthenoteratozoospermia characterized by absent, short, coiled, angulation, and irregular-caliber flagella. Genetic variants DNAH1 gene have been identified as a causative factor multiple intracytoplasmic injection available strategy for infertile males with dynein axonemal heavy chain 1 defects to conceive.
Which genetic variants might explain the causes of empty follicle syndrome (EFS) and abnormal zona pellucida (ZP) affect success treatment with assisted reproductive technologies (ART)?Whole-exome sequencing was performed in probands EFS ZP. Sanger used for variant validation. Using HEK-293T cells, effects ZP1 ZP2 on protein expression were explored by western blotting, effect location investigated via immunofluorescence. The structure also analysed to investigate pathogenicity variants.A...
Abstract Background The global delay in women’s reproductive age has raised concerns about age-related infertility. decline oocyte quality is a limiting factor of female fertility, yet there are currently no strategies to preserve aged women. Here, we investigated the effects growth hormone (GH) supplementation on aneuploidy oocytes. Methods For vivo experiments, mice (8-month-old) were intraperitoneally injected with GH daily for 8 weeks. vitro germinal vesicle oocytes from treated during...
Abstract STUDY QUESTION Could actin-related protein T1 (ACTRT1) deficiency be a potential pathogenic factor of human male infertility? SUMMARY ANSWER A 110-kb microdeletion the X chromosome, only including ACTRT1 gene, was identified as responsible for infertility in two Chinese males with sperm showing acrosomal ultrastructural defects and fertilization failure. WHAT IS KNOWN ALREADY The proteins (e.g. ACTRT1, ACTRT2, ACTL7A, ACTL9) interact each other to form multimeric complex...
Abstract Postovulatory aging leads to the decline in oocyte quality and subsequent impairment of embryonic development, thereby reducing success rate assisted reproductive technology (ART). Potential preventative strategies preventing oocytes from associated underlying mechanisms warrant investigation. In this study, we identified that cordycepin, a natural nucleoside analogue, promoted vitro, as indicated by reduced fragmentation, improved spindle/chromosomes morphology mitochondrial...
Vitrification is an important way to cryopreserve human embryos and the recommended time of embryo exposure vitrification solution 1 min. However, practically speaking, duration equilibration can vary from 5 15 The purpose this study was investigate effect different times on outcomes frozen-thawed transfer cycles. data were collected our medical records January 2012 June 2013 a total 517 cycles included. These divided into four groups according time: (i) 5-6 min; (ii) 7-8 (iii) 9-10 min (iv)...
Abstract Study question Is there any novel variants and mutant hotspot in DNAH1 gene related to multiple morphological abnormalities of sperm flagella (MMAF) male infertility humans? Summary answer We identified eighteen eleven MMAF-affected Chinese men, including fourteen variants, four previously reported a candidate hotspot. What is known already As specific type asthenoteratozoospermia, MMAF characterized by composite flagellar defects absent, short, coiled, angulation,...
Abstract Background Along with progress in embryo cryopreservation, especially the vitrification, freeze all strategy has become more acceptable than ever. Some studies have found comparable or higher live birth rate frozen transfer (FET) fresh transfer(ET)in gonadotropin releasing hormone antagonist (GnRH-ant) protocol. However from our literature research, there been no reports about comparison between ET and FET agonist (GnRH-a) long The aim of this study is to retrospectively investigate...
This study was to evaluate whether Intracytoplasmic sperm injection (ICSI) can improve the quality of embryo in patients with poor-quality embryos previous In-vitro fertilization (IVF) cycle, which cancelled before transfer. a retrospective cohort 178 IVF and 158 ICSI cycles for cycle Center Reproductive Medicine, Women Children's Hospital Chongqing Medical University from March 2016 June 2022. The 2 PN rate, oocyte utilization rate , high-quality clinical pregnancy were compared between two...
The genotype-phenotype relationships between TUBB8 variants and female infertility are difficult to clearly define due the complex inheritance patterns highly heterogeneous phenotypes. This study aims identify novel relevant phenotypes in more infertile females. A total of 35 females with primary were recruited from two reproductive centers investigated for identifying TUBB8. Pedigree analysis, in-silico analysis molecular remodeling performed assess their clinical significance. effects on...
Summary This study aimed to evaluate what extent the different interval times between trophectoderm (TE) biopsy and vitrification influence clinical outcomes in preimplantation genetic testing (PGT) cycles. Patients who underwent frozen embryo transfer (FET) after PGT 2015 2019 were recruited. In total, 297 cycles with single day 5 euploid blastocyst included. These divided into three groups according times: <1 h group, 1–2 ≥2 group. Blastocyst survival, pregnancy, miscarriage, ongoing...
To explore the correlation between blastomere count variations "skip value" which extracted from by time-lapse technology (TLT) combined with artificial intelligence (AI) and morphological features of in vitro fertilization (IVF) embryo, to test its feasibility clinical applications.
Abstract This study aimed to demonstrate the utilization value of 1PN embryos. The zygotes collected from December 2021 September 2022 were included in this study. embryo development, pronuclear characteristics, and genetic constitutions investigated. overall blastocyst formation good-quality rates 22.94 16.24%, significantly lower than those 2PN (63.25 50.23%, respectively, P = 0.000). characteristics found be correlated with developmental potential. When comparing that developed into...