Maria C. Vladoiu
A5067051713- Glioma Diagnosis and Treatment
- Single-cell and spatial transcriptomics
- Galectins and Cancer Biology
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Glycosylation and Glycoproteins Research
- Genomics and Chromatin Dynamics
- Cancer Genomics and Diagnostics
- Toxin Mechanisms and Immunotoxins
- Chromatin Remodeling and Cancer
- RNA modifications and cancer
- RNA Research and Splicing
- Hedgehog Signaling Pathway Studies
- CAR-T cell therapy research
- Circular RNAs in diseases
- Spectroscopy Techniques in Biomedical and Chemical Research
- MicroRNA in disease regulation
- Spectroscopy and Chemometric Analyses
- Neurogenesis and neuroplasticity mechanisms
- Phytoplasmas and Hemiptera pathogens
- interferon and immune responses
- Hippo pathway signaling and YAP/TAZ
- Fetal and Pediatric Neurological Disorders
- Meningioma and schwannoma management
- Immune cells in cancer
University of Toronto
2018-2025
Hospital for Sick Children
2017-2025
SickKids Foundation
2018-2025
McMaster University
2024
Centre Hospitalier de l’Université de Montréal
2018
McGill University
2017
Brain Tumour Research
2017
Institut National de la Recherche Scientifique
2014-2017
Montreal Neurological Institute and Hospital
2017
Cancer Institute (WIA)
2017
Abstract Existing RNA velocity estimation methods strongly rely on predefined dynamics and cell-agnostic constant transcriptional kinetic rates, assumptions often violated in complex heterogeneous single-cell sequencing (scRNA-seq) data. Using a graph convolution network, DeepVelo overcomes these limitations by generalizing to cell populations containing time-dependent kinetics multiple lineages. infers time-varying cellular rates of transcription, splicing, degradation, recovers each cell’s...
Targeting oncogenic pathways holds promise for brain tumor treatment, but inhibition of Sonic Hedgehog (SHH) signaling has failed in SHH-driven medulloblastoma. Cellular diversity within tumors and reduced lineage commitment can undermine targeted therapy by increasing the probability treatment-resistant populations. Using single-cell RNA-seq tracing, we analyzed cellular medulloblastomas transgenic, medulloblastoma-prone mice, responses to SHH-pathway inhibitor vismodegib. In untreated...
Abstract Sonic hedgehog medulloblastoma encompasses a clinically and molecularly diverse group of cancers the developing central nervous system. Here, we use unbiased sequencing transcriptome across large cohort 250 tumors to reveal differences among molecular subtypes disease, demonstrate previously unappreciated importance non-coding RNA transcripts. We identify alterations within cAMP dependent pathway ( GNAS , PRKAR1A ) which converge on GLI2 activity show that 18% have genetic event...
Abstract Many genes that drive normal cellular development also contribute to oncogenesis. Medulloblastoma (MB) tumors likely arise from neuronal progenitors in the cerebellum, and we hypothesized heterogeneity observed MBs with sonic hedgehog (SHH) activation could be due differences developmental pathways. To investigate this question, here perform single-nucleus RNA sequencing on highly differentiated SHH extensively nodular histology malignant cells resembling each stage of canonical...
We identify a population of Protogenin-positive (PRTG
Objective To test if Raman spectroscopy ( RS ) is an appropriate tool for the diagnosis and possibly grading of prostate cancer PC a). Patients Methods Between 20 50 spectra were acquired from 32 fresh non‐processed post‐prostatectomy specimens using a macroscopic handheld probe. Each measured area was characterized categorized according to histopathological criteria: tissue type (extraprostatic or prostatic); malignancy (benign malignant); grade (Grade Groups [ GG s] 1–5); glandular level....
Medulloblastoma (MB) is a neoplasm linked to dysregulated cerebellar development. Previously, we demonstrated that the Sonic Hedgehog (SHH) subgroup grows hierarchically, with Sox2+ cells at apex of tumor progression and relapse. To test whether this mechanism rooted in normal developmental process, studied role Sox2 We find external germinal layer (EGL) derived from embryonic precursors EGL maintains rare fraction during first postnatal week. Through lineage tracing single-cell analysis,...
Human cerebral cancers are known to contain cell types resembling the varying stages of neural development. However, basis this association remains unclear. Here, we map development mouse cerebrum across developmental time-course, from embryonic day 12.5 postnatal 365, performing single-cell transcriptomics on >100,000 cells. By comparing reference atlas data >100 glial tumours adult and paediatric human cerebrum, find that tumour cells have an expression signature overlaps with temporally...
// Andrée-Anne Grosset 1, 2 , Marilyne Labrie 1 Maria Claudia Vladoiu Einas M Yousef Louis Gaboury Yves St-Pierre INRS-Institut Armand-Frappier, Laval, Quebec H7V 1B7, Canada IRIC | Université de Montréal, Montreal, H3T 1J4, Correspondence to: St-Pierre, e-mail: yves.st-pierre@iaf.inrs.ca Keywords: breast cancer, triple negative, galectins, tissue microarrays, immunohistochemistry Received: November 05, 2015 Accepted: January 29, 2016 Published: February 27, ABSTRACT Because of their ability...
// Marilyne Labrie 1 , Maria Claudia Vladoiu Andrée-Anne Grosset Louis Gaboury 2 and Yves St-Pierre INRS-Institut Armand-Frappier, Laval, Québec, Canada Institute for Research in Immunology Cancer, Downtown Station, Montréal, Correspondence: St-Pierre, email: Keywords : galectin-7, ovarian cancer, immunosupression, p53, MMP-9 Received June 09, 2014 Accepted July 31, Published Abstract There is a critical need to develop effective new strategies diagnosis treatment of cancer. In the present...
Abstract OTX2 is a potent oncogene that promotes tumor growth in Group 3 medulloblastoma. However, the mechanisms by which represses neural differentiation are not well characterized. Here, we perform extensive multiomic analyses to identify an regulatory network controls medulloblastoma cell fate. silencing modulates repressive chromatin landscape, decreases levels of PRC2 complex genes and increases expression neurodevelopmental transcription factors including PAX3 PAX6 . Expression...
Galectins are small soluble lectins that bind α-galactosides via their carbohydrate recognition domain (CRD). Their ability to dimerize is critical for the crosslinking of glycoprotein receptors and subsequent cellular signaling. This particularly important in immunomodulatory role induction T-cell apoptosis. Because galectins play a central many pathologies, including cancer, they represent valuable therapeutic targets. At present, most inhibitors have been directed towards CRD, challenging...
The observation that galectin-7 (gal-7) is specifically expressed in mammary myoepithelial (basal) cells prompted us to investigate whether this protein the basal of other tissues. Given breast and prostate cancer have remarkable underlying biological similarities given important roles cancer, we examined expression patterns role gal-7 human cancer. Using tissue microarray, found although readily normal tissue, it downregulated (PCa) cells. De novo increases their sensitivity apoptosis...
Summary The study of the origin and development cerebellar tumours has been hampered by complexity heterogeneity cells that change over course development. We used single-cell transcriptomics to >60,000 from developing murine cerebellum, show different molecular subgroups childhood tumors mirror transcription distinct, temporally restricted lineages. Sonic Hedgehog medulloblastoma transcriptionally mirrors granule cell hierarchy as expected, whereas Group 3 resemble Nestin +ve stem cells,...