Christopher Dunham
A5059000453- Glioma Diagnosis and Treatment
- Radiomics and Machine Learning in Medical Imaging
- Cancer Genomics and Diagnostics
- Chromatin Remodeling and Cancer
- Neuroblastoma Research and Treatments
- Fetal and Pediatric Neurological Disorders
- Sarcoma Diagnosis and Treatment
- Neurofibromatosis and Schwannoma Cases
- Hedgehog Signaling Pathway Studies
- Cancer Mechanisms and Therapy
- Ferroptosis and cancer prognosis
- Cancer-related molecular mechanisms research
- Microtubule and mitosis dynamics
- Meningioma and schwannoma management
- Brain Metastases and Treatment
- Neonatal and fetal brain pathology
- Autopsy Techniques and Outcomes
- Vascular Malformations Diagnosis and Treatment
- Spinal Dysraphism and Malformations
- Vasculitis and related conditions
- Neurogenetic and Muscular Disorders Research
- Head and Neck Surgical Oncology
- MicroRNA in disease regulation
- RNA Research and Splicing
- Gestational Trophoblastic Disease Studies
University of British Columbia
2016-2025
Children's & Women's Health Centre of British Columbia
2015-2025
British Columbia Children's Hospital
2014-2024
B.C. Women's Hospital & Health Centre
2024
Syracuse University
2023-2024
Deleted Institution
2022
University of Calgary
2006-2021
Wayne State University
2020
Howard Hughes Medical Institute
2018
University of California, Santa Cruz
2018
Infant gliomas have paradoxical clinical behavior compared to those in children and adults: low-grade tumors a higher mortality rate, while high-grade better outcome. However, we little understanding of their biology therefore cannot explain this nor what constitutes optimal management. Here report comprehensive genetic analysis an international cohort clinically annotated infant gliomas, revealing 3 subgroups. Group 1 arise the cerebral hemispheres harbor alterations receptor tyrosine...
Posterior fossa ependymoma comprises two distinct molecular variants termed EPN_PFA and EPN_PFB that have a biology natural history. The therapeutic value of cytoreductive surgery radiation therapy for posterior after accounting subgroup is not known.Four independent nonoverlapping retrospective cohorts ependymomas (n = 820) were profiled using genome-wide methylation arrays. Risk stratification models designed based on known clinical newly described biomarkers identified by multivariable...
Purpose High-grade gliomas (HGGs; WHO grades 3-4) are highly diverse, with survival times ranging from months to years. 2000 grading criteria for high-grade oligodendroglial neoplasms [anaplastic oligoastrocytoma (AOA) and anaplastic oligodendroglioma (AO)] remain subjective, the existence of grade 4 variants is controversial. Patients Methods Overall (OS) 1,093 adult patients a cerebral HGG newly diagnosed between 1990 2005 was analyzed by univariate multivariate models significance...
Abstract Objective Spinal muscular atrophy (SMA) is an inherited neuromuscular disorder leading to paralysis and subsequent death in young children. Initially considered a motor neuron disease, extra‐neuronal involvement increasingly recognized. The primary goal of this study was investigate alterations lipid metabolism SMA patients mouse models the disease. Methods We analyzed clinical data collected from large cohort pediatric type I–III as well I liver necropsy data. In parallel, we...
We sought to investigate clinical outcomes of relapsed medulloblastoma and compare molecular features between patient-matched diagnostic tumors.Children infants enrolled on either SJMB03 (NCT00085202) or SJYC07 (NCT00602667) trials who experienced relapse were analyzed for outcomes, including anatomic temporal patterns postrelapse survival. A largely independent, paired cohort was by DNA methylation array next-generation sequencing.A total 72 329 (22%) 52 79 (66%) patients with significant...
Background: Clinical trials of anti-Aβ monoclonal antibodies in Alzheimer disease (AD) infer target engagement from Aβ positron emission tomography (PET) and/or fluid biomarkers such as cerebrospinal (CSF) Aβ42/40.However, these measure deposits indirectly incompletely.In contrast, postmortem neuropathologic assessments allow direct investigation treatment effects on brain and many other pathologic features.Methods: From a clinical trial dominantly inherited AD, we measured...
West Nile virus (WNV) infection causes neurological disease at all levels of the neural axis, accompanied by neuroinflammation and neuronal loss, although underlying mechanisms remain uncertain. Given substantial activation neuroinflammatory pathways observed in WNV infection, we hypothesized that WNV-mediated cell death occurred through both glia neurons, which was driven part capsid protein expression. Analysis autopsied tissues from humans with encephalomyelitis (WNVE) revealed neurons...
Malignant peripheral nerve sheath tumors (MPNST) are highly aggressive sarcomas with variable patient survival and few known prognostically relevant genomic biomarkers. To identify survival-associated biomarkers, we performed high-resolution array-based comparative hybridization (aCGH) on a large set of MPNSTs.Candidate gene alterations identified by aCGH in 38 MPNSTs were validated at the DNA, RNA, protein levels these same an independent 87 MPNST specimens.aCGH revealed complex copy number...
Abstract Medulloblastoma is the most common malignant brain tumor in children. This disease heterogeneous and composed of four subtypes medulloblastoma [WNT, Sonic Hedgehog (SHH), Group 3, 4]. An immediate goal to identify novel molecular targets for aggressive forms medulloblastoma. Polo-like kinase 1 (PLK1) an oncogenic that controls cell cycle proliferation, making it a strong candidate treatment. In this study, pediatric medulloblastomas were subtyped two patient cohorts (discovery...
Aims DNA methylation‐based central nervous system (CNS) tumour classification has identified numerous molecularly distinct types, and clinically relevant subgroups among known CNS entities that were previously thought to represent homogeneous diseases. Our study aimed at characterizing a novel, defined variant of glioneuronal tumour. Patients methods methylation profiling was performed using the Infinium MethylationEPIC or 450 k BeadChip arrays (Illumina) analysed ‘conumee’ package in R...
Childhood posterior fossa group A ependymomas demonstrate integrated metabolic and epigenetic pathways that can be disrupted by metformin.
Abstract The role for routine whole genome and transcriptome analysis (WGTA) poor prognosis pediatric cancers remains undetermined. Here, we characterize somatic mutations, structural rearrangements, copy number variants, gene expression, immuno-profiles germline cancer predisposition variants in children adolescents with relapsed, refractory or malignancies who underwent WGTA matched sequencing. Seventy-nine participants a median age at enrollment of 8.8 y (range 6 months to 21.2 y) are...
We identify a population of Protogenin-positive (PRTG
Angiomatoid fibrous histiocytoma (AFH) is generally considered a soft tissue sarcoma of low malignant potential that occurs in children/young adults and most frequently affects the extremities. AFH infrequently recurs rarely metastasizes. has characteristic histomorphology, immunohistochemical reactivities for desmin CD68 have led to myofibroblastic fibrohistiocytic histogenetic hypotheses, respectively. Although only limited number cases been molecularly characterized, many demonstrated...
Glioblastoma multiforme (GBM) ranks among the deadliest types of cancer and given these new therapies are urgently needed. To identify molecular targets, we queried a microarray profiling 467 human GBMs discovered that polo-like kinase 1 (PLK1) was highly expressed in tumors it clustered with proliferative subtype. Patients PLK1-high were more likely to die from their disease suggesting current inactive against such tumors. This prompted us examine its expression brain tumor initiating cells...
Abstract Brain tumors represent the leading cause of childhood cancer mortality, which medulloblastoma (MB) is most frequent malignant tumor. Recent studies have demonstrated presence several MB molecular subgroups, each distinct in terms prognosis and predicted therapeutic response. Groups 1 2 are characterized by relatively good clinical outcomes activation Wnt Shh pathways, respectively. In contrast, groups 3 4 (“non-Shh/Wnt MBs”) distinguished metastatic disease, poor patient outcome,...
While international consensus and the 2021 WHO classification recognize multiple molecular medulloblastoma subgroups, these are difficult to identify in clinical practice utilizing routine approaches. As a result, biology-driven risk stratification therapy assignment for remains major challenge. Here, we report mass spectrometry-based analysis of samples subgroup discovery, highlighting MYC-driven prognostic signature MYC immunohistochemistry (IHC) as clinically tractable method improved...
Molecular profiling of tumors has proven to be a valuable tool for identification prognostic and diagnostic subgroups in medulloblastomas, glioblastomas, other cancers. However, the molecular landscape atypical teratoid/rhabdoid (AT/RTs) remains largely unexplored. To address this issue, we used microarrays measure gene expression profiles 18 AT/RTs performed unsupervised hierarchical clustering determine molecularly similar subgroups. Four major (clusters) were identified. These did not...
Abstract Background Rhabdoid tumors (RTs) are aggressive of early childhood that occur most often in brain (AT/RTs) or kidney (KRTs). Regardless location, they characterized by loss functional SMARCB1 protein, a component the SWI/SNF chromatin remodeling complex. The aim this study was to determine genes and biological process dysregulated common both AT/RTs KRTs. Procedure Gene expression for compared other normal using microarray data from our lab. Similar analysis performed KRTs GEO....