A5077817320- Sarcoma Diagnosis and Treatment
- Peptidase Inhibition and Analysis
- PARP inhibition in cancer therapy
- Histone Deacetylase Inhibitors Research
- Lymphoma Diagnosis and Treatment
- Protein Degradation and Inhibitors
- CAR-T cell therapy research
- Cancer, Hypoxia, and Metabolism
- Synthesis of Tetrazole Derivatives
- Synthesis and biological activity
- Ferrocene Chemistry and Applications
- Cancer-related molecular mechanisms research
- Cancer Research and Treatments
- Economic and Financial Impacts of Cancer
- Advancements in Semiconductor Devices and Circuit Design
- Cancer, Lipids, and Metabolism
- Signaling Pathways in Disease
- Beetle Biology and Toxicology Studies
- Low-power high-performance VLSI design
- RNA modifications and cancer
- RNA Research and Splicing
- Radiation Effects in Electronics
- Cancer Genomics and Diagnostics
- Diet and metabolism studies
- Click Chemistry and Applications
BC Cancer Agency
2021-2024
University of British Columbia
2021-2023
Terry Fox Research Institute
2023
Abstract Cancer cells must overcome anoikis (detachment-induced death) to successfully metastasize. Using proteomic screens, we found that distinct oncoproteins upregulate IL1 receptor accessory protein (IL1RAP) suppress anoikis. IL1RAP is directly induced by oncogenic fusions of Ewing sarcoma, a highly metastatic childhood sarcoma. inactivation triggers and impedes dissemination sarcoma cells. Mechanistically, binds the cell-surface system Xc− transporter enhance exogenous cystine uptake,...
Ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1) is a type II transmembrane glycoprotein expressed in many tissues. High expression levels of ENPP1 have been observed cancer types such as lung cancer, ovarian and breast cancer. Such overexpression has associated with poor prognosis these diseases. Hence, potential target for immunotherapy across multiple cancers. Here, we isolated characterized two high-affinity specific anti-ENPP1 Fab antibody candidates, 17 3G12, from large...
Abstract Purpose: Histone deacetylase (HDAC) inhibition has been shown to induce pharmacologic “BRCAness” in cancer cells with proficient DNA repair activity. This provides a rationale for exploring combination treatments HDAC and PARP types that are insensitive single-agent inhibitors (PARPi). Here, we report the concept characterization of novel bifunctional PARPi (kt-3283) dual activity toward PARP1/2 enzymes Ewing sarcoma cells. Experimental Design: Inhibition was measured using PARP1/2,...
While international consensus and the 2021 WHO classification recognize multiple molecular medulloblastoma subgroups, these are difficult to identify in clinical practice utilizing routine approaches. As a result, biology-driven risk stratification therapy assignment for remains major challenge. Here, we report mass spectrometry-based analysis of samples subgroup discovery, highlighting MYC-driven prognostic signature MYC immunohistochemistry (IHC) as clinically tractable method improved...
Abstract Purpose: Ewing sarcoma is the second most common bone in children, with 1 case per 1.5 million United States. Although survival rate of patients diagnosed localized disease approximately 70%, this decreases to 30% for metastatic and only 10% treatment-refractory disease, which have not changed decades. Therefore, new therapeutic strategies are urgently needed refractory sarcoma. Experimental Design: This study analyzed 19 unique patient- or cell line–derived xenografts (from 14...
Given that ketogenic diets (KDs) are extremely high in dietary fat, we compared different fats KDs to determine which was the best for cancer prevention. Specifically, a Western and 15% carbohydrate diet seven KDs, containing either or enriched medium chain fatty acids (MCTs), milk fat (MF), palm oil (PO), olive (OO), corn (CO) fish (FO) their ability reduce nicotine-derived nitrosamine ketone (NNK)-induced lung mice. While all tested were more effective at reducing nodules than diet, FO-KD...
Effective therapies for metastatic osteosarcoma (OS) remain a critical unmet need. Targeting mRNA translation in OS offers promising option, as selective drives the synthesis of cytoprotective proteins under harsh microenvironmental conditions to facilitate competence.
<div>AbstractPurpose:<p>Ewing sarcoma is the second most common bone in children, with 1 case per 1.5 million United States. Although survival rate of patients diagnosed localized disease approximately 70%, this decreases to 30% for metastatic and only 10% treatment-refractory disease, which have not changed decades. Therefore, new therapeutic strategies are urgently needed refractory Ewing sarcoma.</p>Experimental Design:<p>This study analyzed 19 unique patient- or...
Abstract We recently showed that a low-carbohydrate (CHO) diet containing soy protein and fish oil dramatically reduces lung nodules in mouse model of cancer when compared to Western diet. To explore the universality this finding, we herein low-CHO on preventing breast prostate using expresses SV40 large T-antigen specifically epithelia females males. found was significantly reduced with correlated reduction plasma levels glucose, insulin, IL-6, TNFα prostaglandin E2 (PGE2). This also...
<p>Scoring schema to prioritize candidate immunotherapy targets.</p>
<p>ENPP1 immunofluorescence.</p>
<p>Expression of ATP11C, SLCO5A1, and STEAP2 in publicly available datasets.</p>
<p>Domain structure of ENPP1 and CDH11.</p>
<p>Statistical analysis (DEqMS) of proteins, EwS vs SuperMix</p>
<p>CDH11 expression in three publicly available datasets.</p>
<p>ENPP1 immunofluorescence.</p>
<p>Characteristics of proteins in the scoring schema.</p>
<p>Characteristics of proteins in the scoring schema.</p>
<p>Scoring schema to prioritize candidate immunotherapy targets.</p>
<p>Expression of known EwS surface proteins in cell line models and publicly available data.</p>
<p>Sample clustering of surface proteome data.</p>
<p>Sample clustering of surface proteome data.</p>
<p>Peptide coverage of all proteins and surface in the global surfaceome approaches.</p>