A5061024018- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- IL-33, ST2, and ILC Pathways
- Immune Cell Function and Interaction
- Hepatitis B Virus Studies
- CAR-T cell therapy research
- Glycosylation and Glycoproteins Research
- Escherichia coli research studies
- Antibiotic Resistance in Bacteria
- Bacterial Infections and Vaccines
- Peptidase Inhibition and Analysis
- Radiopharmaceutical Chemistry and Applications
- Eosinophilic Esophagitis
- Galectins and Cancer Biology
- Protein purification and stability
- Eosinophilic Disorders and Syndromes
- Immune Response and Inflammation
- HER2/EGFR in Cancer Research
- Vibrio bacteria research studies
- Antimicrobial Peptides and Activities
- Virology and Viral Diseases
- Pneumonia and Respiratory Infections
- Toxoplasma gondii Research Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- Occupational and environmental lung diseases
Xuzhou Medical College
2025
Nanjing Drum Tower Hospital
2025
University of Pittsburgh
2021-2024
University of Pittsburgh Medical Center
2023
Chinese Academy of Medical Sciences & Peking Union Medical College
2014-2019
Peking Union Medical College Hospital
2014-2019
Academy of Medical Sciences
2019
Key Laboratory of Guangdong Province
2019
Institute of Medical Biology
2014-2016
Institut de Recherche Vaccinale
2014
Abstract Outer membrane vesicles (OMVs) have proven to be highly immunogenic and induced an immune response against bacterial infection in human clinics animal models. We sought investigate whether engineered OMVs can a feasible antigen-delivery platform for efficiently inducing specific antibody responses. In this study, Omp22 (an outer protein of A. baumannii ) was displayed on E. coli DH5 α -derived (Omp22-OMVs) using recombinant gene technology. The morphological features Omp22-OMVs were...
Acinetobacter baumannii is considered the prototypical example of a multi- or pan- drug-resistant bacterium. It has been increasingly implicated as major cause nosocomial and community-associated infections. This study proposed to evaluate efficacy immunological approaches prevent treat A. infections.Mice were immunized with outer membrane vesicles (OMVs) prepared from clinically isolated multidrug-resistant strain baumannii. Pneumonia sepsis models used active passive immunization OMVs. The...
Abstract A. baumannii infections are becoming more and serious health issues with rapid emerging of multidrug extremely drug resistant strains, therefore, there is an urgent need for the development nonantibiotic-based intervention strategies. This study aimed at identifying whether outer membrane protein molecular weight about 22 kDa (Omp22) holds potentials to be efficient vaccine candidate combat infection. Omp22 which has a molecule length 217 amino acids kept than 95% conservation in...
Ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1) is a type II transmembrane glycoprotein expressed in many tissues. High expression levels of ENPP1 have been observed cancer types such as lung cancer, ovarian and breast cancer. Such overexpression has associated with poor prognosis these diseases. Hence, potential target for immunotherapy across multiple cancers. Here, we isolated characterized two high-affinity specific anti-ENPP1 Fab antibody candidates, 17 3G12, from large...
<title>Abstract</title> Objective This study aimed to elucidate the relationship between gut microbiota and metabolomic profiles in patients with systemic lupus erythematosus (SLE) nephritis (LN) identify potential biomarkers their roles disease progression. Methods Fecal samples from 15 healthy controls (HC) 36 SLE (18 SLE-nonLN 18 SLE-LN) were analyzed using 16S rRNA gene sequencing untargeted metabolomics. Differential microbial taxa metabolites identified Linear Discriminant Analysis...
Therapeutic human papillomavirus (HPV) vaccines are currently being developed. However, no therapeutic efficacy has been achieved in clinical trials for the treatment of cervical intraepithelial neoplasia or cancer. One important issues increasing vaccine is determining best way to enhance tumor antigen-specific cellular immune responses. This study aimed explore virus-like particles (VLPs) hepatitis B core antigen (HBcAg) as potential carriers and assess its immunological...
Neutrophil elastase (NE) is a serine protease released during neutrophil maturation. High levels of NE are related to lung tissue damage and poor prognosis in cancer; thus, potential target for therapeutic immunotherapy multiple diseases cancers. Here, we isolate characterize two high-affinity, specific, noncompetitive anti-NE antibodies Fab 1C10 VH 1D1.43 from large phage-displayed human libraries. After fusion with IgG1 Fc, both them (VH-Fc 1C10) inhibit enzymatic activity VH-Fc showing...
We sought to develop an IL-33 vaccine and evaluate its efficacy in a mouse model of asthma. The full-length molecules putative mature were inserted into the immunodominant epitope region hepatitis B core antigen using gene recombination techniques. expressed chimeric protein presented as virus-like particles (VLPs) under observation electron microscopy. To investigate immunization characteristics VLPs, mice immunized by different doses, adjuvants, routes. VLPs induced sustained high titers...
Cross-talk by pattern recognition receptors may facilitate the maturation of dendritic cells and fine tune immune response. Thus, inclusion ligands agonistic to multiple in a vaccine formula be an effective strategy elicit robust antitumor cellular immunity. We tested adjuvant effects possible synergy CpG (CpG oligodeoxynucleotide), Poly I:C (polyinosinic-polycytidylic acid) cationic peptide Cramp (cathelicidin-related antimicrobial peptide) formulated DOTAP...
Mesothelin (MSLN) has been a validated tumor-associated antigen target for several solid tumors over decade, making it an attractive option therapeutic interventions. Novel antibodies with high affinity and better properties are needed. In the current study, we have isolated characterized novel heavy chain variable (VH) domain 3C9 from large-size human immunoglobulin VH library. exhibited (KD [dissociation constant] <3 nM) binding specificity in membrane proteome array (MPA). mouse xenograft...
Mesothelin (MSLN) is a tumor-associated antigen found in variety of cancers and target for imaging therapeutic applications MSLN-expressing tumors. We have developed high affinity anti-MSLN human VH domain antibodies, providing alternative targeting vectors to conventional IgG antibodies that are associated with long-circulating half-lives poor penetration tumors, limiting antitumor activity clinical trials. Based on two newly identified binders (3C9, 2A10), we generated VH-Fc fusion...
The elevated expression of GPNMB and VCAM-1 has been observed in many cancers including breast cancer, melanoma, prostate cancers. Such overexpression associated with poor prognosis increased cancer metastasis. Thus, are potential targets for immunotherapies across multiple In this study, two high-affinity specific human VH domain antibody candidates, 87 (GPNMB) 1B2 (VCAM-1), were isolated from our in-house proprietary phage-displayed libraries. avidity was after conversion to VH-Fc....
The high expression of uPAR has been linked to tumor progression, invasion, and metastasis in several types cancer. Such overexpression makes it a potential target for immunotherapies across common cancers such as breast, colorectal, lung, ovarian cancer, melanoma. In our study, two high-affinity specific human VH domain antibody candidates, designed clones 3 115, were isolated from phage-displayed library. Domain-based bispecific T- cell engagers (DbTE) based on these antibodies exhibited...
Cathelicidin has been reported to be multifunctional. The current study aimed investigate the influences of exogenous cathelicidin-related antimicrobial peptide (CRAMP) on inflammatory responses in different disease models. In OVA-induced allergic airway inflammation, CRAMP significantly enhanced infiltration cells and accumulation proinflammatory Th2 cytokine IL-13 IL-33 bronchial alveolar lavage fluid (BALF), exacerbated lung tissue inflammation goblet cell hyperplasia, elevated...
Therapeutic vaccine appears to be a potential approach for the treatment of human papillomavirus (HPV)-associated tumours, but its efficacy can dampened by immunosuppressive factors such as transforming growth factor (TGF)-β1. We sought investigate whether active immunity against TGF-β1 enhances anti-tumour elicited an HPV16 E7-specific that we developed previously. In this study, virus-like particles hepatitis B virus core antigen were used carriers deliver either cell epitopes or E7...
Prostate stem cell antigen (PSCA) is expressed in all stages of prostate cancer, including advanced androgen-independent tumors and bone metastasis. PSCA may associate with carcinogenesis lineage plasticity cancer. also a promising theranostic marker for variety other solid tumors, pancreatic adenocarcinoma renal carcinoma. Here, we identified novel fully human antibody using phage display methodology. The structure-based affinity maturation yielded high-affinity binder, F12, which highly...
Abstract Antibody based therapeutics targeting mesothelin (MSLN) have shown limited anticancer activity in clinical trials. Novel antibodies with high affinity and better therapeutic properties are needed. In the current study, we isolated characterized a novel VH domain 3C9 from large size human immunoglobulin heavy chain variable (VH) library. exhibited [KD (dissociation constant) < 3 nM] binding specificity membrane proteome array (MPA). mouse xenograft model, fused to Fc became...
Recently, interleukin (IL)-33 has been closely associated witha variety of clinical cancers. IL-33 presents both protumorigenic, and less frequently, antitumorigenic functions depending on disease conditions. signaling appears to be a possible target for the treatment applicable tumor diseases. This study aimed develop an effective approach intervene in functioning tumors reveal immunotherapeutic potential anti-IL-33 active immunization. Recombinant truncated hepatitis B virus core antigen...