A5058074481- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Ferroptosis and cancer prognosis
- CRISPR and Genetic Engineering
- Cancer Immunotherapy and Biomarkers
- Retinal Development and Disorders
- Virus-based gene therapy research
- Retinal Diseases and Treatments
- T-cell and B-cell Immunology
- Tryptophan and brain disorders
- Gut microbiota and health
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Cancer, Hypoxia, and Metabolism
- RNA regulation and disease
- Diet and metabolism studies
- Pharmacological Effects of Natural Compounds
- Retinal Imaging and Analysis
- Monoclonal and Polyclonal Antibodies Research
- Molecular Biology Techniques and Applications
- Mesenchymal stem cell research
- Retinopathy of Prematurity Studies
Soochow University
2024-2025
Nanjing Drum Tower Hospital
2020-2025
Guangdong Pharmaceutical University
2015-2025
Xuzhou Medical College
2025
Jiangsu Provincial Center for Disease Control and Prevention
2018-2024
Nanjing Medical University
2016-2024
First Affiliated Hospital of Soochow University
2024
Bioscience (China)
2024
Jiangsu Province Hospital
2016-2022
Southern Medical University
2013-2022
Over the past two decades, immunotherapies have increasingly been considered as first-line treatments for most cancers. One such treatment is immune checkpoint blockade (ICB), which has demonstrated promising results against various solid tumors in clinical trials. Monoclonal antibodies (mAbs) are currently available inhibitors (ICIs). These ICIs target specific checkpoints, including cytotoxic T-lymphocyte-associated antigen-4 (CTLA-4) and programmed cell death protein 1 (PD-1). Clinical...
In the present report, we characterize a monoclonal antibody directed at surface differentiation antigen on human T cells. The antibody, OKT17, recognizes cell majority of resting normal peripheral contrast, OKT17 is unreactive with B cells, lines, or SIg+ CLL. Interestingly, after activation, recognized by lost from subset OKT4+ We took advantage this finding to explore further functional heterogeneity within activated Evidence was obtained that PWM-activated remaining depletion...
Premature ovarian insufficiency (POI) is clinically irreversible in women aged over 40 years. Although numerous studies have demonstrated satisfactory outcomes of mesenchymal stem cell therapy, the underlying therapeutic mechanism remains unclear. Exosomes were collected from culture medium human umbilical cord cells (hUMSCs) and assessed by electron microscopy Western blot (WB) analysis. Then, exosomes added to cyclophosphamide (CTX)-damaged granulosa (hGCs), mixture was injected into...
Objective Dysregulation of transfer RNA (tRNA)-derived small noncoding (tsRNA) signatures in human serum has been found various diseases. Here, we determine whether the tsRNAs can serve as biomarkers for diagnosis or prognosis systemic lupus erythematosus (SLE). Methods Initially, sequencing was employed screening obtained from SLE patients, followed by validation with TaqMan probe-based quantitative reverse transcription-PCR (RT-PCR) assay. Receiver operating characteristic (ROC) curve...
Tumor immune evasion relies on the crosstalk between tumor cells and adaptive/innate cells. Immune checkpoints play critical roles in crosstalk, checkpoint inhibitors have achieved promising clinical effects. The long non-coding RNA taurine-upregulated gene 1 (TUG1) is upregulated hepatocellular carcinoma (HCC). However, how TUG1 effects are incompletely understood. Here, METTL3-mediated m
CCL17 is an important chemokine that plays a vital immunomodulatory role in the tumor microenvironment (TME). Analysis of lung adenocarcinoma (LUAD) data Kaplan-Meier plotter databases found overall survival patients high-expression group was higher than low-expression group, especially for with early (stages I and II) LUAD, which has more positive prognostic value. Expression LUAD positively correlated proportion tumor-infiltrating lymphocytes, immunostimulators, major histocompatibility...
Changes in bacteriome composition have a strong association with gastric cancer (GC). However, the relationship between stomach fungal microbiota and human host immune factors remains largely unknown. With high-throughput internal transcribed spacer region 2 (ITS2) sequencing, we characterized microbiome among GC ( n = 22), matched para-GC healthy individuals 11). A total of 4.5 million valid tags were generated stratified into 1,631 operational taxonomic units (OTUs), 10 phyla 301 genera...
Pseudomonas aeruginosa is an opportunistic human pathogen notorious for its remarkable capacity of multi-drug resistance, and has become one the most important model bacteria in clinical bacteriology research. Quantitative real-time PCR a reliable method widely used gene expression analysis, which selection set appropriate housekeeping genes key prerequisite accuracy results. However, it easy to overlook that level may vary different conditions, especially condition molecular microbiology...
<title>Abstract</title> Objective This study aimed to elucidate the relationship between gut microbiota and metabolomic profiles in patients with systemic lupus erythematosus (SLE) nephritis (LN) identify potential biomarkers their roles disease progression. Methods Fecal samples from 15 healthy controls (HC) 36 SLE (18 SLE-nonLN 18 SLE-LN) were analyzed using 16S rRNA gene sequencing untargeted metabolomics. Differential microbial taxa metabolites identified Linear Discriminant Analysis...
Background: Adoptive cell therapy (ACT), including CAR-T and TCR-T therapies, shows promise for cancer treatment, depending on infused T expansion, persistence activity. We previously characterized four T-cell subsets (TN, TSCM, TCM TEM) their miRNA profiles. Objectives: This study investigates miR-143-3p’s role in differentiation. Methods: Using qPCR, we analyzed miR-143-3p expression. Target genes were validated by dual-luciferase assays. Functional assays assessed differentiation markers,...
Introduction Despite recent developments in our comprehension of how the gut microbiota and systemic lupus erythematosus (SLE) are related. The mycobiome: which is a small but crucial part involved hosts’ homeostasis physiological processes, remained unexplored SLE. Methods We profiled fungal mycobiota based on internal transcribed spacer region 1 (ITS1) sequencing for microbial DNA from SLE individuals with nephritis (LN) ( n = 23), without LN 26) healthy controls 14) enrolled Nanjing Drum...
Abstract Background Human amniotic epithelial cell (hAEC) transplantation holds great promise in treating premature ovarian insufficiency (POI). However, some deficient biological characteristics of hAECs restrict their application. Methods Vitamin C (VC) was added to the culture media for 2 weeks. Then, proliferative ability, migration pluripotency, and self-renewal VC-treated (VC-hAECs) were determined. Next, VC-hAECs transplanted into ovaries cyclophosphamide (CTX)-induced POI model mice....
Cancer-associated fibroblasts (CAFs) are heterogeneous constituents of the tumor microenvironment involved in tumorigenesis, progression, and therapeutic responses tumors. This study identified four distinct CAF subtypes breast cancer (BRCA) using single-cell RNA sequencing (RNA-seq) data. Of these, matrix CAFs (mCAFs) were significantly associated with remodeling strongly correlated transforming growth factor (TGF)-β signaling pathway. Consensus clustering The Cancer Genome Atlas (TCGA)...
Context: The recent developments in non-viral gene therapy and DNA vaccine have fostered the development of efficient plasmid (pDNA) purification processes.Objectives: This work aimed to establish a cost-effective process for large-scale production vaccine.Materials methods: E. coli DH5α harboring pCDNA3.1-GFP (7200 base pairs) was used as model plasmid. Hydrophobic-interaction chromatography (HIC) employed purify supercoiled (sc pDNA).Results: With this method, not only host contaminants,...
Development of the CRISPR-Cas9 gene-editing system has given rise to a new era gene editing with wide applications in biology, medicine, agriculture, and other fields. However, overexpression Cas9 nuclease causes off-target effects may trigger an immune response vivo. Therefore, we constructed self-restricting system, where target sequence corresponding guide RNA (gRNA) is inserted on either end promoter. When double-strand breaks (DSBs) are induced sequence, promoter cut off transcription...
Purpose: We verified whether fetal RPE (fRPE) cells and mesenchymal stem (MSCs) cotransplantation can combine the features of these two cell types alleviate retinal degeneration in a degenerative disease mouse model. Methods: Tail vein injection sodium iodate (NaIO3) was conducted to establish MSCs fRPE were transplanted either separately or combined subretinal space animals. ERG, optical coherence tomography, histologic, immunofluorescence analyses performed. Furthermore, expression level...