A5025238504- Glioma Diagnosis and Treatment
- Childhood Cancer Survivors' Quality of Life
- Acute Lymphoblastic Leukemia research
- Neuroblastoma Research and Treatments
- Single-cell and spatial transcriptomics
- Acute Myeloid Leukemia Research
- interferon and immune responses
- Chemotherapy-induced cardiotoxicity and mitigation
- HIV Research and Treatment
- Malaria Research and Control
- Complement system in diseases
- Ferroptosis and cancer prognosis
- Immunotherapy and Immune Responses
- Ethics and Legal Issues in Pediatric Healthcare
- Immune Cell Function and Interaction
- Pancreatic and Hepatic Oncology Research
- Ethics in medical practice
- Ethics in Clinical Research
- Monoclonal and Polyclonal Antibodies Research
- Hematopoietic Stem Cell Transplantation
- Reproductive System and Pregnancy
- Ocular Oncology and Treatments
- Viral-associated cancers and disorders
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Caveolin-1 and cellular processes
Royal Children's Hospital
2016-2025
Murdoch Children's Research Institute
2016-2025
The University of Melbourne
2007-2024
University of Pittsburgh
2024
The Royal Melbourne Hospital
2012-2014
Burnet Institute
2010
Northwestern University
2008-2009
NorthShore University HealthSystem
2008
Peter MacCallum Cancer Centre
2005-2008
The immune response of macrophage cells to internalized polyvalent nucleic acid-functionalized gold nanoparticles has been studied. This study finds that the innate (as measured by interferon-β levels) densely functionalized, oligonucleotide-modified is significantly less (up a 25-fold decrease) when compared lipoplex carrying same DNA sequence. magnitude this effect inversely proportional oligonucleotide density. It proposed enzymes involved in recognizing foreign acids and triggering are...
Abstract Acute lymphoblastic leukemia (ALL) is the most common childhood malignancy, and implementation of risk-adapted therapy has been instrumental in dramatic improvements clinical outcomes. A key to therapies includes identification genomic features individual tumors, including chromosome number (for hyper- hypodiploidy) gene fusions, notably ETV6-RUNX1, TCF3-PBX1, BCR-ABL1 B-cell ALL (B-ALL). RNA-sequencing (RNA-seq) large cohorts expanded recurrent fusions recognized as drivers ALL,...
Background: Clinical trials of anti-Aβ monoclonal antibodies in Alzheimer disease (AD) infer target engagement from Aβ positron emission tomography (PET) and/or fluid biomarkers such as cerebrospinal (CSF) Aβ42/40.However, these measure deposits indirectly incompletely.In contrast, postmortem neuropathologic assessments allow direct investigation treatment effects on brain and many other pathologic features.Methods: From a clinical trial dominantly inherited AD, we measured...
orn et al 6 (Lund, accession no.
Pediatric high-grade gliomas (pHGGs) are the most aggressive brain tumors in children, necessitating innovative therapies to improve outcomes. Unlike adult gliomas, recent research reveals that childhood have distinct biological features, requiring specific treatment strategies. Here, we focused on deciphering unique genetic dependencies gliomas. Using a pooled CRISPR/Cas9 knockout screening approach 65 pediatric and 10 glioma (HGG) cell lines, myeloid leukemia 1 (MCL1) emerged as key...
Abstract Tumorigenic drivers of MYCN gene nonamplified neuroblastoma remain largely uncharacterized. Long noncoding RNAs (lncRNAs) regulate tumorigenesis, however, there is little literature on therapeutic targeting lncRNAs with small molecule compounds. Here PRKCQ‐AS1 identified as the lncRNA most overexpressed in nonamplified, compared ‐amplified, cell lines. expression controlled by super‐enhancers, and RNA bound to MSI2 protein. immunoprecipitation sequencing BMX mRNA transcript...
Abstract Background Antibody opsonization of Plasmodium falciparum -infected erythrocytes (IE) plays a crucial role in anti-malarial immunity by promoting clearance blood-stage infection monocytes and macrophages. The effects phagocytosis opsonized IE on macrophage pro-inflammatory cytokine responses are poorly understood. Methods Phagocytic clearance, response intracellular signalling were measured using IFN-γ-primed human monocyte-derived macrophages (MDM) incubated with unopsonized...
Emerging viruses in the paramyxovirus genus Henipavirus evade host antiviral responses via protein interactions between viral V and W proteins cellular STAT1 STAT2 cytosolic RNA sensor MDA5. Polo-like kinase (PLK1) is identified as being an additional partner that can bind to Nipah virus P, V, proteins. For both Hendra virus, contact PLK1 polo box domain required for phosphorylation. Results indicate engaged by amino acids 100 160, previously binding responsible interferon (IFN) signaling...
HIV-1 infection increases the risk and severity of malaria by poorly defined mechanisms. We investigated effect HIV-1Ba-L monocyte-derived macrophages (MDM) on phagocytosis opsonised P. falciparum infected erythrocytes (IE) subsequent proinflammatory cytokine secretion. Compared to mock-infected MDM, significantly inhibited IE (median (IQR) (10 (0–28) versus (34 (27–108); internalised/100 MDM; p = 0.001) decreased secretion IL-6 (1,116 (352–3,387) 1,552 (889–6,331); pg/mL; 0.0078) IL-1β (16...
Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a rare and aggressive hematological malignancy with median survival of 14 months (with window 6–28 months) from diagnosis.1 BPDCN derived the precursors cells2 recognized as an independent entity myeloid neoplasms in 2016 updated World Health Organization classification.3 Although predominantly affects adults age 70s, cases involving younger children have been reported. Unique clinical presentations include skin infiltration, at...
The binding by HIV-1 gp120 to CD4 and a chemokine receptor activates the membrane fusion glycoprotein, gp41. function of gp41 involves refolding its core into 6-helix bundle, which apposes lipophilic termini (the peptide transmembrane domain) associated cell viral membranes, leading their fusion. In this study, we examined functional role polar segment proximal external region (MPER), link domain, respectively, domain interact form terminal clasp adjacent core. Limited proteolysis indicated...
HIN-200 proteins are interferon (IFN)-inducible that can regulate cell proliferation and differentiation in vitro. Characterization of the lineage type-dependent expression Ifi202 revealed little or no Lin−/c-Kit+ fraction enriched for immature hematopoietic progenitor cells (HPCs) but higher levels more differentiated Lin−/c-Kit− Lin+ populations. The highest were observed CD11b+/Gr-1dim granulocytes bone marrow. In peripheral blood, was expressed only myeloid lineage, with level seen...
Abstract Cancer therapy related cardiac dysfunction (CTRCD) is an area of increasing focus, particularly during the survivorship period, for paediatric, adolescent and adult cancer survivors. With advent immunotherapy targeted therapy, there a new set mechanisms from which paediatric young patients with may suffer cardiovascular injury. Furthermore, disease leading cause morbidity mortality in period. The recently established Australian Cardio‐Oncology Registry largest only population‐based...
Immunopathology of placental malaria is most significant in women their first pregnancy especially endemic areas, due to a lack protective immunity Plasmodium falciparum, which acquired successive pregnancies. In some studies (but not all), grand multigravidae (defined as 5 or more pregnancies, G5–7) are susceptible poor birth outcomes associated with compared earlier gravidities. By comparing peripheral cellular responses primigravidae (G1), second fourth (G2–4) and we sought identify key...
Abstract HIN‐200 proteins are interferon‐inducible capable of regulating cell growth, senescence, differentiation and death. Using a combination in silico analysis NCBI EST databases screening murine C57BL/6 cDNA libraries we isolated novel cDNAs designated Ifi206 S L encoding two putative mRNA splice variants. The p206 protein isoforms have modular domain structure consisting an N‐terminal PAAD/DAPIN/Pyrin domain, region rich serine, threonine proline residues C‐terminal 200 B...
It has long been established that cardiotoxicity occurs as a result of exposure to certain chemotherapeutics, particularly anthracyclines. Historically, clinicians equate with poor prognosis, in small percentage patients and deem long-term surveillance optional. Emerging evidence suggests anthracycline (ACT) is life-long risk an incidence approaching 20%.To elucidate the within current paediatric oncology survivor cohort.Participants were identified through Haematology-Oncology database at...
<h3>Objectives</h3> This study aimed to determine the ability successfully contact past paediatric patients and their families request participation in research, assess familial views on use of previously collected archival clinical samples for research purposes, highlight ethical practical issues obtaining this type retrospective consent. <h3>Methods</h3> To such we contacted a cohort with children diagnosed brain tumour ask consent an epigenetic/genetic study. Examining participants9...
Abstract Background A key component of cancer research is the availability clinical samples with appropriately annotated data. Biobanks facilitate by collecting/storing various types for research. Brain Cancer Biobanking Australia (BCBA) was established to networking brain biobanking operations Australia-wide. Maximizing biospecimen utility in a networked environment requires standardization procedures and data across different sites. The aim this scope develop recommended annotation dataset...