A5038141767- HIV Research and Treatment
- Immune Cell Function and Interaction
- Complement system in diseases
- Malaria Research and Control
- T-cell and B-cell Immunology
- HIV/AIDS Research and Interventions
- HIV-related health complications and treatments
- Mosquito-borne diseases and control
- Cytomegalovirus and herpesvirus research
- Hepatitis B Virus Studies
- Research on Leishmaniasis Studies
- Chromosomal and Genetic Variations
- interferon and immune responses
- Monoclonal and Polyclonal Antibodies Research
- Salivary Gland Disorders and Functions
- Cancer Research and Treatments
- Reproductive System and Pregnancy
- Oral microbiology and periodontitis research
- Diverse Scientific Research Studies
- Perovskite Materials and Applications
- vaccines and immunoinformatics approaches
- Blood properties and coagulation
- Advanced Glycation End Products research
- Immune cells in cancer
- Influenza Virus Research Studies
RMIT University
2017-2024
Chinese Academy of Sciences
2009-2024
University of Chinese Academy of Sciences
2024
Institute of High Energy Physics
2024
MIT University
2024
John Wiley & Sons (United States)
2022
East China University of Science and Technology
2000-2018
Burnet Institute
2009-2017
Institut Pasteur of Shanghai
2009
An understanding of the mechanisms mediating protective immunity against malaria in humans is currently lacking, but critically important to advance development highly efficacious vaccines. Antibodies play a key role acquired immunity, functional basis for their effect remains unclear. Furthermore, there strong need immune correlates protection guide vaccine development. Using validated assay measure opsonic phagocytosis Plasmodium falciparum merozoites, we investigated potential this...
Glucose metabolism plays a fundamental role in supporting the growth, proliferation and effector functions of T cells. We investigated impact HIV infection on key processes that regulate glucose uptake primary CD4 CD8
Human immunodeficiency virus (HIV) persistence in blood and tissue reservoirs, including the brain, is a major barrier to HIV cure possible cause of comorbid disease. However, size replication competent nature central nervous system (CNS) reservoir unclear. Here, we used intact proviral DNA assay (IPDA) provide first quantitative assessment defective brain people with (PWH).Total, intact, proviruses were measured autopsy frontal lobe from viremic (n = 18) or virologically suppressed 12) PWH....
Monocyte activation during HIV-1 infection is associated with increased plasma levels of inflammatory markers and risk for premature development age-related diseases. Because activated monocytes primarily use glucose to support cellular metabolism, we hypothesized that chronic monocyte induces a hypermetabolic response uptake. To test this hypothesis, evaluated transporter 1 (Glut1) expression uptake by subpopulations in HIV-seropositive (HIV(+)) treatment-naive individuals (n = 17), HIV(+)...
We previously demonstrated that NK cells from HIV-infected individuals have elevated expression of activation markers, spontaneously degranulate ex vivo, and decrease a signal-transducing protein for NK-activating receptors, FcRγ. Importantly, these changes were maintained in virologically suppressed (VS) receiving combination antiretroviral therapy (cART). In this study, we show loss FcRγ is caused by the expansion novel subset FcRγ(-)CD56(dim) with an altered receptor repertoire biological...
HIV persistence in the brain is a barrier to cure, and potentially contributes HIV-associated neurocognitive disorders. Whether transcription persists despite viral suppression with antiretroviral therapy (ART) subject same blocks seen other tissues blood, unclear. Here, we quantified level of transcripts frontal cortex tissue from virally suppressed or non-virally people (PWH). transcriptional profiling (and PBMCs where available) (n = 11) PWH 13) was performed using digital polymerase...
HIV-infected (HIV+) individuals have an increased risk of atherosclerosis and cardiovascular disease which is independent antiretroviral therapy traditional factors. Monocytes play a central role in the development atherosclerosis, HIV-related chronic inflammation monocyte activation may contribute to but mechanisms are unknown.Using in-vitro model atherosclerotic plaque formation, we measured transendothelial migration purified monocytes from age-matched HIV+ uninfected donors examined...
With more than 600,000 deaths from malaria, mainly of children under five years old and caused by infection with Plasmodium falciparum, comes an urgent need for effective anti-malaria vaccine. Limited details on the mechanisms protective immunity are a barrier to vaccine development. Antibodies play important role in malaria monocytes key effectors antibody-mediated protection phagocytosing antibody-opsonised infected erythrocytes (IE). Eliciting antibodies that enhance phagocytosis IE is...
Here, we provide the first regional analysis of intact and defective HIV reservoirs within brain. Brain tissue from both viremic virally suppressed people with (PWH) harbored pol DNA in all regions tested, lower levels present basal ganglia cerebellum relative to frontal white matter. Intact proviruses were primarily found matter but also detected other brain PWH, demonstrating as a major reservoir intact, potentially replication competent that persists despite antiretroviral therapy. ANN...
Abstract Background Antibody opsonization of Plasmodium falciparum -infected erythrocytes (IE) plays a crucial role in anti-malarial immunity by promoting clearance blood-stage infection monocytes and macrophages. The effects phagocytosis opsonized IE on macrophage pro-inflammatory cytokine responses are poorly understood. Methods Phagocytic clearance, response intracellular signalling were measured using IFN-γ-primed human monocyte-derived macrophages (MDM) incubated with unopsonized...
Abstract Monocytes constitutively migrate from the bloodstream across vascular endothelium for systemic immune surveillance and maintenance of macrophage populations. They also perform reverse transendothelial migration (TEM) endothelium, which is required entry tissue monocytes/macrophages into lymphatics or back bloodstream. We have modeled these processes previously using HUVEC monolayers grown on three-dimensional collagen matrices. The aim present study was to determine whether HIV-1...
Eradication of HIV cannot be achieved with combination antiretroviral therapy (cART) because the persistence long-lived latently infected resting memory CD4+ T cells. We previously reported that latency could established in cells presence chemokine CCL19. To define how CCL19 facilitated establishment latent infection, role receptor signalling was explored. In cells, induced phosphorylation RAC-alpha serine/threonine-protein kinase (Akt), nuclear factor kappa B (NF-κB),...
HIV-1 infection increases the risk and severity of malaria by poorly defined mechanisms. We investigated effect HIV-1Ba-L monocyte-derived macrophages (MDM) on phagocytosis opsonised P. falciparum infected erythrocytes (IE) subsequent proinflammatory cytokine secretion. Compared to mock-infected MDM, significantly inhibited IE (median (IQR) (10 (0–28) versus (34 (27–108); internalised/100 MDM; p = 0.001) decreased secretion IL-6 (1,116 (352–3,387) 1,552 (889–6,331); pg/mL; 0.0078) IL-1β (16...
Background FcRγ is an immunoreceptor tyrosine-based activation motif (ITAM)-signalling protein essential for signaling and monocyte, macrophage NK cell function. Previous study from our laboratory showed that down-regulated in HIV-infected macrophages vitro. expression immune cells present individuals unknown. Methodology/Principal Findings We compared peripheral blood mononuclear isolated HIV-1-infected receiving combination antiretroviral therapy healthy, HIV-1-uninfected individuals. mRNA...
Innate immune dysfunction persists in HIV+ individuals despite effective combination antiretroviral therapy (cART). We recently demonstrated that an adaptive-like CD56dim NK cell population lacking the signal transducing protein FcRγ is expanded individuals. Here, we analyzed a cohort of men who have sex with (MSM, n = 20) at baseline and following 6, 12, 24 months cART compared them uninfected MSM (n 15) to investigate impact on dysfunction. Proportions cells expressing markers early...
HIV is associated with NK cell dysfunction and expansion of adaptive-like cells that persist despite antiretroviral therapy (ART). We investigated the timing perturbations during acute infection impact early ART initiation. PBMCs plasma were obtained from people (PWH; all men who have sex men; median age, 26.0 y) diagnosed Fiebig stages I, II, III, or IV/V. Participants initiated a 3 d after diagnosis, immunophenotyping was performed at diagnosis longitudinally ART. Anti-CMV Abs assessed by...
Maraviroc (MVC) is an allosteric inhibitor of human immunodeficiency virus type 1 (HIV-1) entry, and the only CCR5 antagonist licensed for use as anti-HIV-1 therapeutic. It acts by altering conformation extracellular loops, rendering unrecognizable HIV-1 envelope (Env) glycoproteins. This study aimed to understand mechanisms underlying development MVC resistance in HIV-1-infected patients. To do this, we obtained longitudinal plasma samples from eight subjects who experienced treatment...
Abstract The buried interface of perovskite plays a crucial role in attaining superior performance, partly due to its influence on the structure overlying perovskite. Here, novel buried‐interface strategy is proposed that incorporates A‐site cations (FA + or Rb ) into hole transport layer (HTL) optimize crystallization. introduction results formation as seed at interface, which effectively promotes growth with improved orientation, crystallinity, and rearranged phase distribution....