A5075552954- Malaria Research and Control
- Mosquito-borne diseases and control
- Complement system in diseases
- HIV Research and Treatment
- Spectroscopy Techniques in Biomedical and Chemical Research
- Viral Infections and Vectors
- vaccines and immunoinformatics approaches
- Vector-borne infectious diseases
- Glycosylation and Glycoproteins Research
- SARS-CoV-2 and COVID-19 Research
- Invertebrate Immune Response Mechanisms
- COVID-19 Clinical Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Parasites and Host Interactions
- Global Maternal and Child Health
- SARS-CoV-2 detection and testing
- Computational Drug Discovery Methods
- Neonatal Health and Biochemistry
- Hepatitis Viruses Studies and Epidemiology
- Travel-related health issues
- Advanced biosensing and bioanalysis techniques
- Chronic Lymphocytic Leukemia Research
- Carbohydrate Chemistry and Synthesis
- Biosensors and Analytical Detection
- Viral Infections and Outbreaks Research
Burnet Institute
2013-2022
Monash University
2013-2022
The University of Melbourne
2013-2022
The Royal Melbourne Hospital
2006-2018
Melbourne Health
2018
The Alfred Hospital
2018
Peter Doherty Institute
2017
Walter and Eliza Hall Institute of Medical Research
2008-2014
Papua New Guinea Institute of Medical Research
2010
During blood-stage infection by Plasmodium falciparum, merozoites invade RBCs. Currently there is limited knowledge of cellular and molecular invasion events, no established assays are available to readily measure quantify invasion-inhibitory antibodies or compounds for vaccine drug studies. We report the isolation viable that retain their invasive capacity, at high purity yield, purified filtration highly synchronous populations schizonts. show half-life merozoite capacity after rupture 5...
An understanding of the mechanisms mediating protective immunity against malaria in humans is currently lacking, but critically important to advance development highly efficacious vaccines. Antibodies play a key role acquired immunity, functional basis for their effect remains unclear. Furthermore, there strong need immune correlates protection guide vaccine development. Using validated assay measure opsonic phagocytosis Plasmodium falciparum merozoites, we investigated potential this...
Abstract The development of effective malaria vaccines and immune biomarkers is a high priority for control elimination. Ags expressed by merozoites Plasmodium falciparum are likely to be important targets human immunity promising vaccine candidates, but very few have been studied. We developed an approach assess Ab responses comprehensive repertoire merozoite proteins investigate whether they protective Abs. 91 recombinant proteins, located on the surface or within invasion organelles,...
Substantial evidence indicates that antibodies to Plasmodium falciparum merozoite antigens play a role in protection from malaria, although the precise targets and mechanisms mediating immunity remain unclear. Different malaria induce distinct immunoglobulin G (IgG) subclass responses, but importance of different responses protective is not known factors determining vivo are poorly understood. We examined IgG MSP1-19 (the 19-kDa C-terminal region surface protein 1), MSP2 (merozoite 2), AMA-1...
Background. Antibodies targeting blood stage antigens are important in protection against malaria, but the principle targets remain unclear. Erythrocyte-binding (EBAs) erythrocyte invasion ligands used by merozoites and may be of protective immunity, there limited data examining their potential importance.
ABSTRACT Individuals in areas of Plasmodium falciparum endemicity develop immunity to malaria after repeated exposure. Knowledge the acquisition and nature protective immune responses P. is presently limited, particularly for young children. We examined antibodies (IgM, IgG, IgG subclasses) merozoite antigens their relationship prospective risk children 1 4 years age a region Papua New Guinea. IgG1, IgG3 generally increased with age, were higher active infection, reflected geographic...
New diagnostic tools that can detect malaria parasites in conjunction with other parameters are urgently required. In this study, Attenuated Total Reflection Fourier transform infrared (ATR-FTIR) spectroscopy combination Partial Least Square Discriminant Analysis (PLS-DA) and Regression (PLS-R) have been applied as a point-of-care test for identifying parasites, blood glucose, urea levels whole samples from thick films on glass slides. The specificity the PLS-DA was found to be 98%...
Abstract Antibodies against P . falciparum merozoites fix complement to inhibit blood-stage replication in naturally-acquired and vaccine-induced immunity; however, specific targets of these functional antibodies their importance protective immunity are unknown. Among malaria-exposed individuals, we show that complement-fixing more strongly correlated with than growth quantified using the current reference assay for merozoite vaccine evaluation. We identify antigen-specific associated...
Background Antibodies that inhibit the growth of blood-stage Plasmodium falciparum may play an important role in acquired and vaccine-induced immunity humans. However, acquisition activity these antibodies is not well understood. Methods We tested dialysed serum purified immunoglobulins from Kenyan children adults for inhibition P. vitro using different parasite lines. Serum were measured by ELISA to antigens, extracted schizonts, recombinant merozoite surface protein 1 (42 kDa C-terminal...
Background. How antimalarial antibodies are acquired and maintained during pregnancy boosted after reinfection with Plasmodium falciparum vivax is unknown. Methods. A nested case-control study of 467 pregnant women (136 Plasmodium-infected cases 331 uninfected control subjects) in northwestern Thailand was conducted. Antibody levels to P. merozoite antigens the pregnancy-specific PfVAR2CSA antigen were determined at enrollment (median 10 weeks gestation) throughout until delivery. Results....
ABSTRACT The role of early to intermediate Plasmodium falciparum -induced cellular responses in the development clinical immunity malaria is not well understood, and such have been proposed contribute both risk episodes. To investigate whether P. are able function as predictive correlates parasitological outcomes, we conducted a prospective cohort study children (5 14 years age) residing region Papua New Guinea where endemic Live, intact -infected red blood cells were applied isolated...
Background In a previous Phase 1/2b malaria vaccine trial testing the 3D7 isoform of candidate Merozoite surface protein 2 (MSP2), parasite densities in children were reduced by 62%. However, breakthrough parasitemias disproportionately alternate dimorphic form MSP2, FC27 genotype. We therefore undertook dose-escalating, double-blinded, placebo-controlled 1 healthy, malaria-naïve adults MSP2-C1, containing recombinant forms two families msp2 alleles, and (EcMSP2-3D7 EcMSP2-FC27), formulated...
Individuals repeatedly infected with malaria acquire protection from infection and disease; immunity is thought to be primarily antibody-mediated directed blood-stage infection. Merozoite surface proteins involved in the invasion of host erythrocytes are likely targets protective antibodies. We hypothesized that Papua New Guinean children (n = 206) who high antibody levels two Plasmodium vivax merozoite proteins, Duffy binding protein region II (PvDBPII) 19-kDa C-terminal P. 1 (PvMSP1(19)),...
Abs targeting blood-stage Ags of Plasmodium falciparum are important in acquired immunity to malaria, but major targets remain unclear. The P. reticulocyte-binding homologs (PfRh) key ligands used by merozoites during invasion erythrocytes. PfRh2a and PfRh2b functionally members this family may be protective immunity, their potential role human has not been examined. We expressed eight recombinant proteins covering the entire PfRh2 common region, as well PfRh2a- PfRh2b-specific regions. were...
Acquired antibodies are important in human immunity to malaria, but key targets remain largely unknown. Plasmodium falciparum reticulocyte-binding-homologue-4 (PfRh4) is for invasion of erythrocytes and may therefore be a target protective immunity.IgG IgG subclass-specific responses against different regions PfRh4 were determined longitudinal cohort 206 children Papua New Guinea (PNG). Human tested functional invasion-inhibitory activity, expression by P. isolates sequence polymorphisms...