A5068590504- Spectroscopy Techniques in Biomedical and Chemical Research
- Spectroscopy and Chemometric Analyses
- Algal biology and biofuel production
- Marine and coastal ecosystems
- Metabolomics and Mass Spectrometry Studies
- Malaria Research and Control
- Isotope Analysis in Ecology
- 3D Printing in Biomedical Research
- thermodynamics and calorimetric analyses
- Force Microscopy Techniques and Applications
- Water Quality Monitoring and Analysis
- Meat and Animal Product Quality
- Aquatic Ecosystems and Phytoplankton Dynamics
- Biosensors and Analytical Detection
- Infrared Thermography in Medicine
- Microbial Community Ecology and Physiology
- Mosquito-borne diseases and control
- Bacterial Identification and Susceptibility Testing
- Diatoms and Algae Research
- Yersinia bacterium, plague, ectoparasites research
- Advanced Chemical Sensor Technologies
- Animal Virus Infections Studies
- Digital Imaging for Blood Diseases
- Liver Disease Diagnosis and Treatment
- Liver physiology and pathology
Monash University
2016-2025
Australian Regenerative Medicine Institute
2014-2025
Discovery Institute
2020-2025
Clayton Foundation
2015-2021
Australian Research Council
2021
The University of Melbourne
2021
Victoria College
2014
Northeastern University
2011
Fourier Transform Infrared (FT‐IR) spectroscopy was used to study carbon allocation patterns in response changes nitrogen availability the diatom Chaetoceros muellerii Lemmerman. The results of FT‐IR measurements were compared with those obtained traditional chemical methods. data both and methods showed that starvation led disappearance differences cell constituents growth rates existing between cells cultured at either high [NO 3 − ] or [NH 4 + ]. Irrespective source supplied before...
The ability to detect DNA conformation in eukaryotic cells is of paramount importance understanding how some retain functionality response environmental stress. It anticipated that the B A transition might play a role resistance damage such as heat, desiccation and toxic damage. To this end, conformational detail about molecular structure has been derived primarily from vitro experiments on extracted or synthetic DNA. Here, we report B- A-like change can occur nuclei intact dehydration. This...
Raman spectroscopy and infrared (IR) are both techniques that allow for the investigation of vibrating chemical particles. These provide information not only about particles through identification functional groups spectral analysis so-called "fingerprints", these methods qualitative quantitative analyses substances in sample. Both frequently being used biology medicine diagnosing illnesses monitoring therapy. The type breast cancer found woman is often a malignant tumor, causing 1.38...
New diagnostic tools that can detect malaria parasites in conjunction with other parameters are urgently required. In this study, Attenuated Total Reflection Fourier transform infrared (ATR-FTIR) spectroscopy combination Partial Least Square Discriminant Analysis (PLS-DA) and Regression (PLS-R) have been applied as a point-of-care test for identifying parasites, blood glucose, urea levels whole samples from thick films on glass slides. The specificity the PLS-DA was found to be 98%...
The development of a new fast, portable and reagent-free diagnostic technique for hepatitis B (HBV) C (HCV) viruses would be an enormous benefit to society. Here, we evalulate the ability Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR) spectroscopy combined with multivariate data analysis classify human serum samples based on presence HBV HCV infection. Sera were prepared using three different methodologies: i) depsoited onto glass cover slips, airdried placed ATR crystal....
Coupling synchrotron IR beam to an ATR element enhances spatial resolution suited for high-resolution single cell analysis in biology, medicine and environmental science.
Atomic force microscopy-infrared (AFM-IR) spectroscopy is a powerful new technique that can be applied to study molecular composition of cells and tissues at the nanoscale. AFM-IR maps are acquired using single wavenumber value: they show either absorbance plotted against value or ratio two values. Here, we implement multivariate image analysis generate use this approach resolve subcellular structural information in red blood infected with Plasmodium falciparum different stages development....
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in an unprecedented need for diagnostic testing that is critical controlling the spread of COVID-19. We propose a portable infrared spectrometer with purpose-built transflection accessory rapid point-of-care detection COVID-19 markers saliva. Initially, purified virion particles were characterized Raman spectroscopy, synchrotron (IR) and AFM-IR. A data set comprising 171 spectra from 29 subjects positive SARS-CoV-2 by...
Diagnosing antimicrobial resistance (AMR) remains critical for improving patient survival rates and treatment outcomes. Current antibiotic susceptibility tests (AST) suffer prolonged turnaround times, necessitating a minimum of 24 h results. Attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy emerges as promising phenotypic testing method in bacteriology due to its rapid chemical characterization capability. Here, we present an innovative approach utilizing...
Abstract Raman spectra were obtained from cells of the chlorophyte unicellular eukaryotic alga Dunaliella tertiolecta , which had been grown either under nutrient‐replete conditions or starved nitrogen for 4 days. Spectra rich in bands could all be attributed to chlorophyll a β‐carotene. A cursory examination differences between replete and indicated decline an increase β‐carotene chlorophytes. Unprocessed showed pronounced baseline effects. variety pre‐processing techniques used attempt...
We report the coupling of a portable Raman spectrometer to an acoustic levitation device enable environmental monitoring and potential taxonomic identification microalgae. Spectra living cells were recorded at 785 nm using fiber-optic probe coupled spectrometer. The spectra exhibit excellent signal-to-noise ratio clearly show bands from chlorophyll β-carotene. levitated photobleached microalgae reduction in concentration relative β-carotene after 10 min exposure quartz halogen lamp....
High quality Fourier transform infrared (FTIR) spectra were acquired from living Micrasterias hardyi cells maintained in an IR transparent flow-through cell using a FTIR microscope coupled to synchrotron light source. Spectral maps of living, nutrient-replete showed band intensities consistent with the known location nucleus and chloroplasts. These very similar fixed, air-dried cells. Bands due lipids lowest absorbance region highest chloroplast this trend was reversed for bands attributed...
The increase in polyunsaturated fatty acid (PUFA) consumption has prompted research into alternative resources other than fish oil. In this study, a new approach based on focal-plane-array Fourier transform infrared (FPA-FTIR) microspectroscopy and multivariate data analysis was developed for the characterisation of some marine microorganisms. Cell lipid compositions lipid-rich yeasts collected from Australian coast were characterised comparison to commercially available PUFA-producing...
A new experimental platform for probing nanoscale molecular changes in living bacteria using atomic force microscopy–infrared (AFM–IR) spectroscopy is demonstrated. This near-field technique eminently suited to the study of single bacterial cells. Here, we report its application monitor dynamical occurring cell wall during division Staphylococcus aureus AFM demonstrate and AFM–IR record spectra showing thickening septum . work was followed by an investigation into cells, with particular...
Widespread elimination of malaria requires an ultra-sensitive detection method that can detect low parasitaemia levels seen in asymptomatic carriers who act as reservoirs for further transmission the disease, but is inexpensive and easy to deploy field income settings. It was hypothesized a new based on infrared spectroscopy, shown laboratory have similar sensitivity PCR detection, could prove effective detecting setting using cheap portable units with data management systems allowing them...
The magnitude of infectious diseases in the twenty-first century created an urgent need for point-of-care diagnostics. Critical shortages reagents and testing kits have had a large impact on ability to test patients with suspected parasitic, bacteria, fungal, viral infections. New tests be highly sensitive, specific, easy use provide results rapid time. Infrared spectroscopy, coupled multivariate machine learning algorithms, has potential meet this unmet demand requiring minimal sample...
An in vivo method for predicting the nutrient status of individual algal cells using Raman microspectroscopy is described. spectra 780 nm laser excitation show enhanced bands mainly attributable to chlorophyll a and beta-carotene. The relative intensities beta-carotene changed under nitrogen limitation, with becoming less intense more prominent. Although from N-replete N-starved cell populations varied, each distribution was distinct enough such that multivariate classification methods, as...