A5079782243- Spectroscopy Techniques in Biomedical and Chemical Research
- Spectroscopy and Chemometric Analyses
- thermodynamics and calorimetric analyses
- Advanced X-ray Imaging Techniques
- Advanced Electron Microscopy Techniques and Applications
- X-ray Spectroscopy and Fluorescence Analysis
- Photoreceptor and optogenetics research
- Calcium Carbonate Crystallization and Inhibition
- Retinal Development and Disorders
- Geochemistry and Elemental Analysis
- Heavy metals in environment
- SARS-CoV-2 and COVID-19 Research
- Microbial Fuel Cells and Bioremediation
- Lignin and Wood Chemistry
- Bone Tissue Engineering Materials
- Reproductive Biology and Fertility
- Metabolomics and Mass Spectrometry Studies
- Surface Modification and Superhydrophobicity
- Dental Research and COVID-19
- SARS-CoV-2 detection and testing
- COVID-19 diagnosis using AI
- bioluminescence and chemiluminescence research
- Aerogels and thermal insulation
- Biosensors and Analytical Detection
- Traditional Chinese Medicine Studies
Monash University
2020-2024
International Centre for Genetic Engineering and Biotechnology
2023-2024
Elettra-Sincrotrone Trieste S.C.p.A.
2014-2023
AREA Science Park
2021-2023
Environment Park
2022
University of Trieste
2015-2016
Scuola Internazionale Superiore di Studi Avanzati
2008-2011
Istituto Universitario di Studi Superiori di Pavia
2009
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in an unprecedented need for diagnostic testing that is critical controlling the spread of COVID-19. We propose a portable infrared spectrometer with purpose-built transflection accessory rapid point-of-care detection COVID-19 markers saliva. Initially, purified virion particles were characterized Raman spectroscopy, synchrotron (IR) and AFM-IR. A data set comprising 171 spectra from 29 subjects positive SARS-CoV-2 by...
Abstract Filamentous cable bacteria display long-range electron transport, generating electrical currents over centimeter distances through a highly ordered network of fibers embedded in their cell envelope. The conductivity these periplasmic wires is exceptionally high for biological material, but chemical structure and underlying transport mechanism remain unresolved. Here, we combine high-resolution microscopy, spectroscopy, imaging on individual bacterium filaments to demonstrate that...
We report on an optimized protocol for the digestion of cellular RNA, which minimally affects cell membrane integrity, maintaining substantially unaltered vibrational contributions other macromolecules. The design this allowed us to collect first Fourier transform infrared (FTIR) spectra intact hydrated B16 mouse melanoma cells deprived RNA and highlight in-cell diagnostic spectral features it. Complementing results with FTIR analysis extracted ds-DNA, ss-cDNA isolated nuclei, we verified...
Radiation damage of biological samples remains a limiting factor in high resolution X-ray microscopy (XRM). Several studies have attempted to evaluate the extent and effects radiation damage, proposing strategies minimise or prevent it. The present work aims assess impact soft X-rays on formalin fixed cells systematic manner. novelty this approach resides investigating not only with XRM, as often reported relevant literature topic, but by coupling it two additional independent...
The magnitude of infectious diseases in the twenty-first century created an urgent need for point-of-care diagnostics. Critical shortages reagents and testing kits have had a large impact on ability to test patients with suspected parasitic, bacteria, fungal, viral infections. New tests be highly sensitive, specific, easy use provide results rapid time. Infrared spectroscopy, coupled multivariate machine learning algorithms, has potential meet this unmet demand requiring minimal sample...
<italic>In vitro</italic>FTIRM analysis of primary OSCCs treated with cisplatin and 5-fluorouracil for the drug–cell interaction.
Apoptosis is a strictly regulated cell death mechanism that plays pivotal role in the normal evolution of multicellular organisms. Its misregulation has been associated with many diseases, making its early and reliable detection key point for modern cellular biology. In this paper, we propose use infrared microspectroscopy (IRMS) as label-free methodology apoptotic-related biochemical processes induced on U937 leukemic monocytes by serum starvation CCCP-exposure. The spectroscopic results...
SISSI-Bio is the Chemical and Life Sciences branch of infrared beamline SISSI (Synchrotron Infrared Source for Spectroscopy Imaging) at Elettra-Sincrotrone, Trieste, Italy. The laboratory has always been kept up-to-date with latest equipment Fourier Transform InfraRed (FTIR) analysis currently hosts three endstations: one spectroscopy, microscopy nanospectroscopy. Although synchrotron radiation allows measurements diffraction limit over full IR spectral range, all end stations can...
Maintaining the correct folding of cellular proteins is essential for preserving homeostasis. Protein dishomeostasis, aberrant protein folding, and aggregation are indeed involved in several diseases including cancer, aging-associated, neurodegenerative disorders. Accumulation aggregates can also be induced from a variety stressful conditions, such as temperature increase or oxidative stress. In this work, we monitored by Fourier transform-infrared (FT-IR) microspectroscopy response live...
Abstract Environmental and occupational inhalants may induce a large number of pulmonary diseases, with asbestos exposure being the most risky. The mechanisms are clearly related to chemical composition physical surface properties materials. A combination X-ray fluorescence (μXRF) Fourier Transform InfraRed (μFTIR) microscopy was used chemically characterize compare bodies versus environmental particulates (anthracosis) in lung tissues from exposed control patients. μXRF analyses revealed...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) has resulted in an unprecedented need for diagnostic testing that is critical controlling the spread of COVID‐19. We propose a portable infrared spectrometer with purpose‐built transflection accessory rapid point‐of‐care detection COVID‐19 markers saliva. Initially, purified virion particles were characterized Raman spectroscopy, synchrotron (IR) and AFM‐IR. A data set comprising 171 spectra from 29 subjects positive...
Wood polyphenolic extracts, commonly called tannins, are excellent candidates for the production of bioplastics due to their abundance in nature, commercial availability, and reactivity. In particular, they were tested as wood adhesives with several hardeners, but low moisture resistance rigidity reduced technological interest. present study, we combined regenerated silk (RS) tannin-furanic formulations improve properties. Three-layer plywood glued these fully renewable tannin-silk-furanic...
The knowledge of cell cycle phase distribution is paramount importance for understanding cellular behaviour under normal and stressed growth conditions. This task usually assessed using Flow Cytometry (FC) or immunohistochemistry. Here we report on the use FTIR microspectroscopy in Microfluidic Devices (MD-IRMS) as an alternative technique studying live cells. Asynchronous, S- G0-synchronized B16 mouse melanoma cells were studied by running parallel experiments based MD-IRMS FC Propidium...
In synchrotron facilities, imaging techniques are on high demand from the scientific community. Those related to X-ray microscopy among most prominent ones. Such include scanning transmission x-ray (STXM), full-field (TXM), and coherent diffraction (CDI) which have a wide spectrum of applications ranging clinical biomedical sciences nanotechnology cultural heritage. Their advancement is achieved through specialisation focused studies, often requiring dedicated beamline end-stations. On other...
Background: Although X-ray fluorescence microscopy is becoming a widely used technique for single-cell analysis, sample preparation this remains one of the main challenges in obtaining optimal conditions measurements regime. The information available to researchers on treatment inadequate and unclear, sometimes leading wasted time jeopardizing experiment’s success. Many cell fixation methods have been described, but none them systematically tested declared most suitable synchrotron...
Analysis with DNA-microrrays and real time PCR show that several genes involved in the thyroid hormone cascade, such as deiodinase 2 3 (Dio2 Dio3) are differentially regulated by circadian clock changes of ambient light. The expression level Dio2 adult rats (2–3 months age) kept continuously darkness is modulated up-regulated fold at midday. When diurnal light was on, increased 4–8 a consequent increase related protein detected around nuclei retinal photoreceptors neurons inner outer nuclear...
Silicon Nitride (SiN) membranes have long been the substrate of choice for many different synchrotron techniques at very wavelengths (from IR to hard X-rays), due their ease production, relative robustness even in films <200 nm thickness, and compatibility with standard microfabrication techniques. Here we present a set data referring custom-made Carbide (SiC) windows. We measured SiC surface roughness, mechanical membrane transmission both soft X-rays wavelengths, compared Si3N4, acquired...
Tannin-furanic rigid foams are bio-based copolymers of tannin plant extract and furfuryl alcohol, promising candidates to replace synthetic insulation foams, as for example polyurethanes phenolics, in eco-sustainable buildings thanks their functional properties, such lightness the material fire resistance. Despite relevance environmental-friendly alternatives petroleum derivatives, many aspects polymerization chemistry still remain unclear. One open issues is on spatial heterogeneity foam,...
Tannin-furanic foams are a promising and biogenic alternative to oil-based porous materials. Their hydrophilic character, typically indicated by contact angle water of 70°, limits some potential applications (for instance outdoor thermal insulation). To overcome this, post-synthetic surface modification step with different fluorine-free organosilanes at 323 K was investigated focus on the final, hydrophobic performance. On one side, methyltrimethoxysilane, 3-(chloropropyl)trimethoxysilane,...
Enzyme immobilization is a key strategy to expand the scope of enzyme applications and enable recycling biocatalysts, resulting in greener more cost-efficient processes.
Radiation damage is an important aspect to be considered when analysing biological samples with X-ray techniques as it can induce chemical and structural changes in the specimens. This work aims provide new insights into soft induced radiation of complete sample, including not only tissue itself but also substrate embedding medium, fixation procedure. Sample preparation handling involves unavoidable interaction sample matrix could play role radiation-damage mechanism. To understand influence...