A5006541709- Mast cells and histamine
- Occupational and environmental lung diseases
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Eosinophilic Disorders and Syndromes
- Asthma and respiratory diseases
- Immune Response and Inflammation
- Immune cells in cancer
- Food Allergy and Anaphylaxis Research
- Lipid Membrane Structure and Behavior
- Cellular transport and secretion
- Cell Adhesion Molecules Research
- Trace Elements in Health
- Erythrocyte Function and Pathophysiology
- Immune Cell Function and Interaction
- Cancer Research and Treatments
- Neonatal Respiratory Health Research
- Eosinophilic Esophagitis
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Iron Metabolism and Disorders
- Aluminum toxicity and tolerance in plants and animals
- Occupational exposure and asthma
- Nitric Oxide and Endothelin Effects
- Cellular Mechanics and Interactions
University of Trieste
2016-2025
University College London
2015
International School of Trieste
1995
University of Verona
1982
To cite this article: Elishmereni M, Alenius HT, Bradding P, Mizrahi S, Shikotra A, Minai-Fleminger Y, Mankuta D, Eliashar R, Zabucchi G, Levi-Schaffer F. Physical interactions between mast cells and eosinophils: a novel mechanism enhancing eosinophil survival in vitro. Allergy 2011; 66: 376–385. Abstract Background: Mast (MCs) eosinophils (Eos) are the key effector of allergic reaction. Although classically associated with different stages response, co-exist inflamed tissue late chronic...
Abstract Mast cell degranulation requires N-ethylmaleimide–sensitive factor attachment protein receptors (SNARE) and mammalian uncoordinated18 (Munc18) fusion accessory proteins for membrane fusion. However, it is still unknown how their interaction supports In this study, we found that small interfering RNA–mediated silencing of the isoform Munc18-2 in mast cells inhibits cytoplasmic secretory granule (SG) release but not CCL2 chemokine secretion. Silencing its SNARE-binding partner...
A neuropathic basis has been suggested for burning mouth syndrome (BMS) and an altered concentration of neuropeptides reported in lingual oral mucosa saliva this disease. The aims study were to compare the levels nerve growth factor (NGF), substance P (SP) degranulation products from mast cells neutrophils BMS subjects with those control subjects.Salivary flow rate, protein concentration, NGF peptide mRNA, SP, tryptase, neutrophil myeloperoxidase calprotectin analyzed 20 age- gender-matched...
Mast cells are key effector in allergic reactions. When activated to degranulate, they release a plethora of bioactive compounds from their secretory granules, including mast cell-restricted proteases such as tryptase. In previous study, we showed that tryptase, addition its intragranular location, can be found within the nuclei where it truncates core histones at N-terminal ends.Considering portions constitute sites for posttranslational modifications major epigenetic impact, evaluated...
The antimycobacterial role of eosinophil peroxidase (EPO), one the most abundant granule proteins in human eosinophils, was investigated. Our data indicate that purified EPO shows significant inhibitory activity towards Mycobacterium tuberculosis H37Rv. On a molar basis, this similar to exhibited by neutrophil myeloperoxidase (MPO) and both dose time dependent. In contrast MPO, which requires H(2)O(2), also anti-M. absence exogenously added peroxide. Morphological evidence confirmed...
Abstract The degranulation of neutrophils and eosinophils is frequently monitored by assaying myeloperoxidase (MPO) eosinophil peroxidase (EPO) activity in the cell-free supernatant degranulating cells, after removal cells centrifugation. This procedure leads to underestimation extent degranulation, since both peroxidases tend stick cell surfaces, test tube walls, particulate stimuli used elicit because their highly cationic nature. In this paper we describe a method for MPO EPO secretion...
We have recently shown that human neutrophils bind and internalize eosinophil peroxidase (EPO) but not myeloperoxidase (MPO). In the present work, we studied interactions of EPO MPO with other cells may be involved in inflammatory process, i.e., lymphocytes, monocytes, platelets, fibroblasts, endothelial cells. The results indicate is bound by all cell types considered, efficiently internalized only Conversely, binds appreciably to fibroblasts cells, although a lower affinity than EPO, its...
Malignant pleural mesothelioma (MPM) is an aggressive cancer with poor prognosis. The development of MPM frequently linked to inhalation asbestos fibers. A genetic component susceptibility this disease suggested by the observation that some individuals develop following lower doses exposure, whereas others exposed higher quantities do not seem be affected. This hypothesis supported also frequent reports familial clustering. Despite widely recognized role iron (Fe) in cellular...
Local disruption of iron homeostasis leading to oxidative stress is considered one the main mechanisms asbestos-dependent cyto-/genotoxicity. Another aspect contributing risk developing pathological consequences upon asbestos exposure due individual genetic fac-tors. In a previous study we identified coding SNP in hephaestin gene (HEPH) exerting protective activity against development related thoracic cancers. Heph fer-roxidase which promotes export concert with permease ferroportin (Fpn1)....
A local disruption of iron homeostasis leading to oxidative stress is considered one the main mechanisms asbestos-related genotoxicity. Another aspect contributing risk developing pathological consequences upon asbestos exposure individual genetic factors. In a previous study, we identified coding SNP in hephaestin gene (HEPH) that protects against thoracic cancer. Heph ferroxidase promotes export concert with permease ferroportin (Fpn1). Here, performed an in-depth functional...
By exploiting the unique characteristics of three ionophores, experimental conditions were found which permit dissociation respiratory stimulation from secretion in polymorphonuclear leucocytes. A marked respiration was produced by ionophore X537A, binds and transports both alkali-earth alkali cations. The stimulatory activity this same at either high or low Na+/K+ ratios medium virtually unaffected extracellular Ca2+. slight oxygen consumption also caused K+-selective valinomycin A23187,...