A5064181453- Cell Adhesion Molecules Research
- Gastric Cancer Management and Outcomes
- Esophageal Cancer Research and Treatment
- Protease and Inhibitor Mechanisms
- Angiogenesis and VEGF in Cancer
- Lymphatic System and Diseases
- Cancer Cells and Metastasis
- Proteoglycans and glycosaminoglycans research
- Colorectal Cancer Surgical Treatments
- Cellular Mechanics and Interactions
- MicroRNA in disease regulation
- Monoclonal and Polyclonal Antibodies Research
- Platelet Disorders and Treatments
- Connective tissue disorders research
- Galectins and Cancer Biology
- Lymphatic Disorders and Treatments
- Lung Cancer Diagnosis and Treatment
- Cancer Immunotherapy and Biomarkers
- Glycosylation and Glycoproteins Research
- Vascular Malformations and Hemangiomas
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Immune Response and Inflammation
- Pregnancy and preeclampsia studies
- Chronic Lymphocytic Leukemia Research
Centro di Riferimento Oncologico
2016-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2016-2025
National Cancer Institute
2013-2017
University of Udine
2000-2012
Molecular Oncology (United States)
2009
National Institutes of Health
2002-2004
Friedrich-Alexander-Universität Erlangen-Nürnberg
2003
University of Trieste
1991-2001
University of Parma
2000
Fetal trophoblast cells invading the decidua in early phase of pregnancy establish complex interaction with maternal extracellular matrix. We discovered that C1q was widely distributed human decidual stroma absence C4 and C3 actively synthesized by migrating extravillous trophoblasts. The expressed messages for three chains secreted this complement component interacted proteins Solid phase-bound promoted adhesion migration, cell binding to resulted activation ERK1/2 MAPKs. Ab inhibition...
EMILINs and Multimerins (EMILIN1, EMILIN2, Multimerin1, Multimerin2) constitute a four member family that in addition to the shared C-terminus gC1q domain typical of gC1q/TNF superfamily members contain N-terminus unique cysteine-rich EMI domain. These glycoproteins are homotrimeric assemble into high molecular weight multimers. They predominantly expressed extracellular matrix contribute several cellular functions part associated with not yet assigned nor linked other specific regions...
Lymphatic-vasculature function critically depends on extracellular matrix (ECM) and its connections with lymphatic endothelial cells (LECs). However, the composition architecture of ECM have not been fully taken into consideration in studying biology pathology system. EMILIN1, an elastic microfibril-associated protein, is highly expressed by LECs vitro colocalizes vessels several mouse tissues. A comparative study between WT Emilin1-/- mice highlighted fact that Emilin1 deficiency both CD1...
Abstract Introduction The increased bone degradation in osteolytic metastases depends on stimulation of mature osteoclasts and continuous differentiation new pre-osteoclasts. Metalloproteinases (MMP)-13 is expressed a broad range primary malignant tumours it emerging as novel biomarker. Recent data suggest direct role MMP-13 dissolving matrix complementing the activity MMP-9 other enzymes. Tumour-microenvironment interactions alter gene expression breast tumour cells promoting metastasis....
EMILIN1 promotes α4β1 integrin–dependent cell adhesion and migration reduces pro–transforming growth factor–β processing. A knockout mouse model was used to unravel functions in skin where the protein abundantly expressed dermal stroma EMILIN1-positive fibrils reached basal keratinocyte layer. Loss of caused epidermal hyperproliferation accelerated wound closure. We identified direct engagement α9β1 integrins as mechanism underlying homeostatic role exerted by EMILIN1. The lack EMILIN1–α4/α9...
We have carried out a comprehensive molecular mapping of PG-M/versican isoforms V0–V3 in adult human tissues and specifically investigated how the expression these is regulated endothelial cells vitro. A survey 21 representative highlighted prevalence V1 mRNA; demonstrated that relative frequency was > V2 V3 ≥ V2; showed <15% transcribed significant levels all four isoforms. By employing novel previously described anti-versican antibodies we verified ubiquitous versican deposition normal...
Lymphatic vasculature plays a crucial role in the maintenance of tissue interstitial fluid balance. The functional collecting lymphatic vessels lymph transport has been recently highlighted pathologies leading to lymphedema, for which treatments are currently unavailable. Intraluminal valves paramount importance this process. However, valve formation and maturation have not entirely elucidated yet, particular, played by extracellular matrix (ECM). We hypothesized that EMILIN1, an ECM...
Colorectal cancer is one of the most frequent and deadly tumors. Among key regulators CRC growth progression, microenvironment has emerged as a crucial player possible route for development new therapeutic opportunities. More specifically, extracellular matrix acts directly on cells indirectly affecting behavior stromal inflammatory cells, well bioavailability factors. ECM molecules, EMILIN-2 frequently down-regulated by methylation in purpose this study was to verify impact loss its value...
Abstract The extracellular matrix (ECM) is an important component of the tumor microenvironment and undergoes extensive remodeling during both initiation progression breast cancer (BC). EMILIN1 ECM glycoprotein, whose function has been linked to metastasis. However, role mammary gland BC development never investigated. In silico molecular analyses human samples from normal showed that expression was lower in tumors than healthy tissue it predicted poor prognosis, particularly HER2-positive...
EMILIN-1 (ElastinMicrofibril Interface Located ProteIN), the prototype of EMILIN family, consists a cysteine-rich domain (EMI domain) at N terminus, an extended region with high potential coiled-coil structure, short collagenous stalk, and self-interacting globular gC1q-l domain. is adhesive extracellular matrix constituent associated elastic fibers, detected also in proximity cell surfaces. To localize attachment site(s), monoclonal antibodies (mAbs) against or gC1q-1 were used to inhibit...
The antimetastatic ruthenium complex imidazolium <i>trans</i>-imidazoledimethylsulfoxide-tetrachlorouthenate (NAMI-A) is tested in the B16 melanoma model vitro and vivo. Treatment of B6D2F1 mice carrying <i>intra</i>-footpad with 35 mg/kg/day NAMI-A for 6 days reduces metastasis weight independently whether given before or after surgical removal primary tumor. Metastasis reduction unrelated to concentration, which 10-fold lower than on site (1 versus 0.1 mM), correlated plasma gelatinolitic...
Osteoclast (OC) precursors migrate to putative sites of bone resorption form functionally active, multinucleated cells. The preOC FLG 29.1 cells, known be capable irreversibly differentiating into OC-like displayed several features primary OCs, including expression specific integrins and the hyaluronan (HA) receptor CD44. cells adhered extensively migrated through membranes coated with fibronectin, vitronectin, laminins, but, although strongly binding HA, totally failed move on this...
Abstract Reactive oxygen species (ROS) are generated as by‐products of respiration and used signal transducing intermediates in out–in signaling pathways. ROS also during inflammatory responses it has been shown that hydrogen peroxide may trigger activation B‐lymphocytes, similar to cross‐linking surface immunoglobulins. On the other hand, both exogenous endogenous a major source nuclear mitochondrial DNA (mtDNA) damage. The base excision repair (BER) enzyme APE/Ref‐1 normally repairs small...
The detection of EMILIN1, a connective tissue glycoprotein associated with elastic fibers, at the level ectoplacental cone and trophoblast giant cells developing mouse embryos (Braghetta et al., 2002) favored idea structural as well functional role for this protein in process placentation. During establishment human placenta, highly migratory subpopulation extravillous trophoblasts (EVT), originating from anchoring chorionic villi, penetrate invade uterine wall. In study we show that...
Heritable connective tissue diseases are a highly heterogeneous family of over 200 disorders that affect the extracellular matrix. While genetic basis several is established, etiology has not been discovered for large portion patients, likely due to rare yet undiscovered disease genes. By performing trio-exome sequencing 55-year-old male proband presenting with multiple symptoms indicative disorder, we identified heterozygous missense alteration in exon 1 Elastin Microfibril Interfacer...
Platinum (PT)-resistant Epithelial Ovarian Cancer (EOC) grows as a metastatic disease, disseminating in the abdomen and pelvis. Very few options are available for PT-resistant EOC patients, little is known about how acquisition of PT-resistance mediates increased spreading capabilities EOC. Here, using isogenic cells, genetic pharmacological approaches, patient-derived models, we report that Integrin α6 (ITGA6) overexpressed by cells necessary to sustain ability adhesion-dependent...
We have identified type VI collagen (Col VI) as a primary subendothelial extracellular matrix component responsible for von Willebrand factor (vWF)-dependent platelet adhesion and aggregation under high tensile strength. Intact tetrameric Col was the form of found to be capable promoting vWF-mediated adhesion/aggregation this shear condition, whereas removal predominant portion terminal globules by pepsin treatment abrogated its activity. The inability pepsin-digested support any interaction...
Production of O2- in response to FMLP, TNF, IFN-gamma, platelet activating factor, LPS, substance P, and PMA by human eosinophils suspension contact with polystyrene ELISA plastic (PL) or biologic surfaces was studied. Monolayers endothelial cells (HEC) PL coated FCS, fibronectin, laminin, collagen types I IV, fibrinogen, fibrin were used as surfaces. Only FMLP stimulated generation suspension. Eosinophils residing on HEC monolayers, either untreated treated unresponsive all stimuli except...
Malignant transformation of epithelial cells is frequently associated with the alteration glycosylation pathways. Tn a common tumor-associated carbohydrate antigen present in 90% human carcinomas and its expression correlates metastatic potential poor prognosis. Despite relevance, functional role tumor biology has not been firmly established probably for lack appropriate experimental tools. Our aims were to produce highly reactive monoclonal antibodies against making use synthetically...