A5084172457- Bacterial biofilms and quorum sensing
- Vibrio bacteria research studies
- Bacterial Genetics and Biotechnology
- Dementia and Cognitive Impairment Research
- Single-cell and spatial transcriptomics
- Molecular Biology Techniques and Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Alzheimer's disease research and treatments
- Cancer Genomics and Diagnostics
Johns Hopkins Medicine
2023-2025
Johns Hopkins University
2023-2025
California University of Pennsylvania
2024
Hospital for Sick Children
2024
Rush University Medical Center
2024
Washington University in St. Louis
2024
Cleveland Clinic
2024
Brigham and Women's Hospital
2024
National Institute on Drug Abuse
2023
Rowan University
2019-2020
Background: Clinical trials of anti-Aβ monoclonal antibodies in Alzheimer disease (AD) infer target engagement from Aβ positron emission tomography (PET) and/or fluid biomarkers such as cerebrospinal (CSF) Aβ42/40.However, these measure deposits indirectly incompletely.In contrast, postmortem neuropathologic assessments allow direct investigation treatment effects on brain and many other pathologic features.Methods: From a clinical trial dominantly inherited AD, we measured...
Many bacteria use quorum sensing (QS) to regulate virulence factor production in response changes population density. QS is mediated through the production, secretion, and detection of signaling molecules called autoinducers (AIs) modulate population-wide behavioral changes. Four histidine kinases, LuxPQ, CqsS, CqsR VpsS, have been identified Vibrio cholerae as receptors activate gene expression at low cell Detection AIs by these leads repression high The redundancy among puzzling since any...
ABSTRACT The pathogen that causes cholera, Vibrio cholerae , uses the cell-cell communication process known as quorum sensing (QS) to regulate virulence factor production and biofilm formation in response changes population density complexity. QS is mediated through detection of extracellular chemical signals called autoinducers. Four histidine kinases, LuxPQ, CqsS, CqsR VpsS, have been identified receptors activate key regulator LuxO at low cell density. At high density, autoinducers by...
Abstract Background Tau pathology is common in age-related neurodegenerative diseases. primary tauopathy (PART) and Alzheimer’s disease (AD) has a similar biochemical structure anatomic distribution, which distinct from tau other However, the molecular changes associated with intraneuronal PART AD, whether these are two diseases, largely unexplored. Methods Using GeoMx spatial transcriptomics, mRNA was quantified CA1 pyramidal neurons adjacent without 6 cases of compared to 4 control...
Abstract Single nucleus RNA-sequencing is critical in deciphering tissue heterogeneity and identifying rare populations. However, current high throughput techniques are not optimized for target populations require tradeoffs design due to feasibility. We provide a novel snRNA pipeline, Muliple X ed Po pulation S election E nrichment snRNA-sequencing ( XPoSE-seq ), enable targeted snRNA-seq experiments in-depth transcriptomic characterization of while retaining individual sample identity.