A5003723350- Neurotransmitter Receptor Influence on Behavior
- Memory and Neural Mechanisms
- Receptor Mechanisms and Signaling
- Neuroscience and Neuropharmacology Research
- Neuroendocrine regulation and behavior
- Analytical Chemistry and Sensors
- Single-cell and spatial transcriptomics
- Electrochemical Analysis and Applications
- Neural dynamics and brain function
- Advanced Fluorescence Microscopy Techniques
- Electrochemical sensors and biosensors
- Photoacoustic and Ultrasonic Imaging
- Neuroinflammation and Neurodegeneration Mechanisms
- Cell Image Analysis Techniques
- Pancreatic function and diabetes
- Engineering Education and Curriculum Development
- Anesthesia and Neurotoxicity Research
- Advanced biosensing and bioanalysis techniques
- Cancer Genomics and Diagnostics
- Engineering Education and Pedagogy
- Retinal Development and Disorders
- Sleep and Wakefulness Research
- Olfactory and Sensory Function Studies
- Heavy Metal Exposure and Toxicity
- Nanoparticles: synthesis and applications
Naval Research Laboratory Information Technology Division
2025
National Institute on Drug Abuse
2015-2025
National Institutes of Health
2015-2023
Purdue University West Lafayette
2012-2020
United States Department of Health and Human Services
2020
Cornell University
2019
Oregon Health & Science University
2019
Marking functionally distinct neuronal ensembles with high spatiotemporal resolution is a key challenge in systems neuroscience. We recently introduced CaMPARI, an engineered fluorescent protein whose green-to-red photoconversion depends on simultaneous light exposure and elevated calcium, which enabled marking active populations single-cell subsecond resolution. However, CaMPARI (CaMPARI1) has several drawbacks, including background low slow kinetics reduced fluorescence after chemical...
Recent studies suggest that the ventral medial prefrontal cortex (vmPFC) encodes both operant drug self-administration and extinction memories. Here, we examined whether these opposing memories are encoded by distinct neuronal ensembles within vmPFC with different outputs to nucleus accumbens (NAc) in male female rats. Using cocaine (3 h/d for 14 d) procedures, demonstrated was similarly activated (indexed Fos) during cocaine-seeking tests after 0 (no-extinction) or 7 sessions. Selective...
Silver nanoparticles (Ag NPs) are gaining popularity as bactericidal agents in commercial products; however, the mechanisms of toxicity (MOT) Ag NPs to other organisms not fully understood. It is goal this research determine differences MOT induced by ionic Ag(+) and Daphnia magna, incorporating a battery traditional novel methods. embryos were exposed sublethal concentrations AgNO3 (130-650 ng/L), with uptake latter confirmed confocal reflectance microscopy. Mitochondrial function was...
Abstract Mapping immediate early gene (IEG) expression across intact mouse brains allows for unbiased identification of brain-wide activity patterns underlying complex behaviors. Accurate registration sample to a common anatomic reference is critical precise assignment IEG-positive (“active”) neurons known brain regions interest (ROIs). While existing automated voxel-based methods provide high-throughput solution, they require substantial computing power, can be difficult implement and fail...
We previously reported that ventral subiculum (vSub) activity is critical to incubation of oxycodone seeking after abstinence induced by adverse consequences drug seeking. Here, we studied the role claustrum, a key vSub input, in this incubation.We trained male and female rats self-administer for 2 weeks then exposing them an electric barrier weeks. used retrograde tracing (cholera toxin B subunit; CTb) plus marker Fos identify projections activated during 'incubated' relapse (abstinence day...
Learning when to initiate or withhold actions is essential for survival and requires integration of past experiences with new information adapt changing environments. While stable prelimbic cortex (PL) ensembles have been identified during reward learning, it remains unclear how they contingencies shift. Does the same ensemble adjust its activity support behavioral suppression upon omission, a distinct recruited this learning? We used single-cell calcium imaging longitudinally track PL...
Abstract Neuronal ensembles in ventromedial prefrontal cortex (vmPFC) play a role both cocaine and palatable food seeking. However, it is unknown whether similar or different vmPFC neuronal mediate Here, we used the Daun02 inactivation procedure to assess mediating seeking can be functionally distinguished. We trained male female Fos‐LacZ rats self‐administer pellets on alternating days for 18 days. then exposed brief nonreinforced food‐ cocaine‐seeking test induce Fos β‐gal associated with...
Studies using rodent models have shown that relapse to drug or food seeking increases progressively during abstinence, a behavioral phenomenon termed “incubation of craving.” Mechanistic studies incubation craving focused on specific neurobiological targets within preselected brain areas. Recent methodological advances in whole-brain immunohistochemistry, clearing, and imaging now allow unbiased brain-wide cellular resolution mapping regions circuits engaged learned behaviors. However, these...
In abstinent drug addicts, cues formerly associated with drug-taking experiences gain relapse-inducing potency ('incubate') over time. Animal models of incubation may help develop treatments to prevent relapse, but these have ubiquitously focused on the role conditioned stimuli (CSs) signaling delivery. Discriminative (DSs) are unique in that they exert stimulus-control both taking and seeking behavior difficult extinguish. For this reason, excitatory effects DSs signal availability, not yet...
Micro-scale brain-machine interface (BMI) devices have provided an opportunity for direct probing of neural function and also shown significant promise restoring neurological functions lost to stroke, injury, or disease. However, the eventual clinical translation such may be hampered by limitations associated with materials commonly used their fabrication, e.g. brittleness silicon, insufficient rigidity polymeric devices, unproven chronic biocompatibility both. Herein, we report, first time,...
Inorganic materials have properties that can be advantageous in bioencapsulation for cell transplantation. Our aim was to engineer a hybrid inorganic/soft tissue construct by inducing pancreatic islets grow an inorganic shell. We created surrounded porous silica, which has potential application the immunoprotection of transplantation therapies type 1 diabetes. The new method takes advantage islet capsule surface as template silica formation. Mouse and human were exposed medium containing...
Surface modification of microelectrodes is a central step in the development microsensors and microsensor arrays. Here, we present an electrodeposition scheme based on voltage pulses. Key features this method are uniformity deposited electrode coatings, flexibility overall deposition area, i.e., sizes number electrodes to be coated, precise control surface texture. Deposition characterization four different materials demonstrated, including layers high-surface-area platinum, gold, conducting...
Abstract Mapping immediate early gene (IEG) expression across intact brains is becoming a popular approach for identifying the brain-wide activity patterns underlying behavior. Registering whole to an anatomical atlas presents technical challenge that has predominantly been tackled using automated voxel-based registration methods; however, these methods may fail when are damaged or only partially imaged, can be challenging correct, and require substantial computational power. Here we present...
Genetically encoded molecular-protein sensors (GEMS) are engineered to sense and quantify a wide range of biological substances events in cells, vitro even vivo with high spatial temporal resolution. Here, we aim stably incorporate these proteins into photopatternable matrix, while preserving their functionality, extend the application as spatially addressable optical biosensors. For this reason, examined fabrication 3D hydrogel microtips doped genetically fluorescent biosensor, GCaMP3, at...
Learning and behavior activate cue-specific patterns of sparsely distributed cells synapses called ensembles that undergo memory-encoding engram alterations. While Fos is often used to label selectively activated cell bodies identify neuronal ensembles, there no comparable endogenous marker synaptic ensembles. For the purpose identifying candidate activity markers, we optimized a flow cytometry synaptoneurosome (FCS) procedure for assessing protein alterations in from male female rats. After...
Recent advances in miniaturization and analyte-sensitive fluorescent indicators make optical fiber biosensors promising alternatives to microelectrodes. Optical sensing offers several advantages over electrochemical methods including increased stability better spatial control monitor physiological processes at cellular resolutions. The distal end of an can be functionalized with different fluorophore/polymer combinations through mechanical, dip-coating or photopolymerization techniques....
A novel method for spatially controlled layer-by-layer assembly of enzyme-based amperometric biosensors was developed multi-analyte sensing with high spatial and temporal resolution. Stereo-electrodes 75 μm tip separation 1-3 size were functionalized using a simple three-step electrodeposition scheme to produce simultaneous detection glucose lactate. Layers nanostructured platinum, enzyme-doped conductive polymer poly(3,4-ethylenedioxythiophene) [PEDOT] non-conductive poly(o-aminophenol)...
Abstract Single nucleus RNA-sequencing is critical in deciphering tissue heterogeneity and identifying rare populations. However, current high throughput techniques are not optimized for target populations require tradeoffs design due to feasibility. We provide a novel snRNA pipeline, Muliple X ed Po pulation S election E nrichment snRNA-sequencing ( XPoSE-seq ), enable targeted snRNA-seq experiments in-depth transcriptomic characterization of while retaining individual sample identity.
Current functional imaging techniques, such as magnetic resonance imaging, rely upon activity-induced blood flood changes to neurons. This indirect measurement of neuronal activity inherently limits image resolution and specificity, however, advances in transgenic technology Photoacoustic (PA) methodology have offered new solutions. We previously demonstrated using PA conjunction with a Fos-LacZ rat model map activated neurons through selective formation PA-active X-gal product within...