A5072173359- Analytical Chemistry and Sensors
- Electrochemical sensors and biosensors
- Electrochemical Analysis and Applications
- Advanced biosensing and bioanalysis techniques
- Spaceflight effects on biology
- Magnetic and Electromagnetic Effects
- Plant responses to water stress
- Light effects on plants
- Plant nutrient uptake and metabolism
- Mechanical and Optical Resonators
- Plant Molecular Biology Research
- Seed Germination and Physiology
- Microfluidic and Bio-sensing Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Plant Reproductive Biology
- Neuroscience and Neural Engineering
- Reproductive Biology and Fertility
- Photosynthetic Processes and Mechanisms
- Pancreatic function and diabetes
- Space Exploration and Technology
- Diatoms and Algae Research
- Plant Stress Responses and Tolerance
- Plant and Biological Electrophysiology Studies
- Cancer, Hypoxia, and Metabolism
- Metabolism, Diabetes, and Cancer
Purdue University West Lafayette
2012-2025
South University
2011-2018
National Aeronautics and Space Administration
2016
Physical Sciences (United States)
2013-2016
University of Science and Technology Beijing
2014
Purdue University System
2013
State Street (United States)
2007-2012
University of Florida
2012
Purdue University Northwest
2011
Weldon City Schools
2011
Abstract Hybridization of nanoscale metals and carbon nanotubes into composite nanomaterials has produced some the best‐performing sensors to date. The challenge remains develop scalable nanofabrication methods that are amenable development with broad sensing ranges. A nanostructured biosensor based on multilayered graphene petal nanosheets (MGPNs), Pt nanoparticles, a biorecognition element (glucose oxidase) is presented. combination zero‐dimensional nanoparticles two‐dimensional support...
Cardiac troponin-I (cTnI) is not found in sera of patients with skeletal muscle disease the absence myocardial injury. It known, however, whether trace amounts cTnI are expressed regenerating human muscle, as has been observed creatine kinase MB. Using immunohistochemical and biochemical techniques, we investigated expression various tissues: heart tissue (n = 5), normal adult 3), fetal 3) obtained, respectively, during transplant, from autopsy, or a bank. Specimens diagnostic biopsies were...
We have isolated and characterized Petunia hybrida cv. Mitchell phenylacetaldehyde synthase (PAAS), which catalyzes the formation of phenylacetaldehyde, a constituent floral scent. PAAS is cytosolic homotetrameric enzyme that belongs to group II pyridoxal 5'-phosphate-dependent amino-acid decarboxylases shares extensive amino acid identity (approximately 65%) with plant L-tyrosine/3,4-dihydroxy-L-phenylalanine L-tryptophan decarboxylases. It displays strict specificity for phenylalanine an...
Nitric oxide (NO) controls diverse functions in many cells and organs of animals. It is also produced plants has a variety effects, but little known about their underlying mechanisms. In the present study, we have discovered role for NO regulation pollen tube growth, fast tip-growing cellular system. Pollen tubes must be precisely oriented inside anatomically complex female ovary order to deliver sperm. We hypothesized that could play this guidance tested hypothesis by challenging growth...
The self-referencing electrode technique was employed to noninvasively measure gradients of dissolved oxygen in the medium immediately surrounding developing mouse embryos and, thereby, characterized changes consumption and utilization during development. A gradient depleted surrounded each embryo could be detected >50 μm from embryo. Blastocysts 0.6 ± 0.1 μM oxygen, whereas early cleavage stage only 0.3 suggesting a twofold increase at blastocyst stage. Mitochondrial oxidative...
Networks of single-walled carbon nanotubes (SWCNTs) decorated with Au-coated Pd (Au/Pd) nanocubes are employed as electrochemical biosensors that exhibit excellent sensitivity (2.6 mA mM−1 cm−2) and a low estimated detection limit (2.3 nM) at signal-to-noise ratio 3 (S/N = 3) in the amperometric sensing hydrogen peroxide. Biofunctionalization Au/Pd nanocube-SWCNT biosensor is demonstrated selective immobilization fluorescently labeled streptavidin on nanocube surfaces via thiol linking....
Indole-3-acetic acid (IAA) is a primary phytohormone that regulates multiple aspects of plant development. Because polar transport IAA an essential determinant organogenesis and dynamic tropic growth, methods to monitor movement in vivo are demand. A self-referencing electrochemical microsensor was optimized non-invasively measure endogenous flux near the surface Zea mays roots without addition exogenous IAA. Enhanced sensor modification, decoupling acquired signals, integrated analyses were...
We report a novel optical biosensor platform using near-infrared fluorescent single-walled carbon nanotubes (SWNTs) functionalized with target-recognizing aptamer DNA for noninvasively detecting cell-signaling molecules in real time. Photoluminescence (PL) emission of aptamer-coated SWNTs is modulated upon selectively binding to target molecules, which exploited detect insulin an insulin-binding (IBA) as molecular recognition element. find that nanotube PL quenches via photoinduced charge...
This work addresses the comparison of different strategies for improving biosensor performance using nanomaterials. Glucose biosensors based on commonly applied enzyme immobilization approaches, including sol–gel encapsulation approaches and glutaraldehyde cross-linking strategies, were studied in presence absence multi-walled carbon nanotubes (MWNTs). Although direct design parameters such as linear range sensitivity is intuitive, this alone not an accurate indicator efficacy, due to wide...
Human space exploration poses inherent risks to astronauts' health, leading molecular changes that can significantly impact their well-being. These alterations encompass genomic instability, mitochondrial dysfunction, increased inflammation, homeostatic dysregulation, and various epigenomic changes. Remarkably, these bear similarities those observed during the aging process on Earth. However, our understanding of connection between shifts disease development in remains limited. Frailty...
Blocking Na x expression improves scarring and atopic dermatitis–like symptoms by improving barrier function.
Abstract Our previous research revealed a key microRNA signature that is associated with spaceflight can be used as biomarker and to develop countermeasure treatments mitigate the damage caused by space radiation. Here, we expand on this work determine biological factors rescued treatment. We performed RNA-sequencing transcriptomic analysis 3D microvessel cell cultures exposed simulated deep radiation (0.5 Gy of Galactic Cosmic Radiation) without antagonists three microRNAs: miR-16-5p,...
Fertilization increases both cytosolic Ca2+ concentration and oxygen consumption in the egg but relationship between these two phenomena remains largely obscure. We have measured mitochondrial NADH on single ascidian eggs found that they increase phase with each series of meiotic waves emitted by pacemakers (PM1 PM2). Oxygen also response to Ins(1,4,5)P3-induced Ca2+transients. Using inhibitors we show active mitochondria sequester during sperm-triggered Ca2+waves are strictly necessary for...
A self-referencing, polarographic, oxygen-selective microelectrode was developed for measuring oxygen fluxes from single cells. This technique is based on the translational movement of at a known frequency through an gradient, between points. The differential current electrode converted into directional measurement flux using Fick equation. Operational characteristics were determined artificial gradients. Calculated values matched theoretical derived static measurements. test preparation,...
We have developed a novel multitip scanning Kelvin probe which can measure changes in biological surface potential ΔVs to within 2 mV and, quasisimultaneously monitor displacement <1 μm. The control and measurement subcomponents are PC based incorporate flexible user interface permitting software of each individual tip, measurement, scan parameters. review the mode operation design features bio-Kelvin including tip steering, signal processing, calibration, tracking/dithering routines....
We describe two hybrid nanomaterial biosensor platforms, based on networks of single-walled carbon nanotubes (SWCNTs) enhanced with Pd nanocubes and Pt nanospheres grown in situ from a porous anodic alumina (PAA) template. These nanocube nanosphere SWCNT are converted into glutamate biosensors by immobilizing the enzyme oxidase (cross-linked gluteraldehyde) onto electrode surface. The nanosphere/SWCNT outperformed nanocube/SWCNT previously reported similar nanomaterial-based amperometrically...